抗体

In this thesis, we carried out a study of antigen synthesis, antibody production and hapten alteration to develop a quicky and sensitive detection method of Quinocetone's residue in animal edible tissues.

本论文通过抗原和抗体的制备及半抗原的改造，进行了在动物可食用组织中喹烯酮残留的ELISA快速检测的方法学研究。

The hapten was conjugated to horseradish peroxidase to prepare enzyme tagged hapten by means of mixed anhydride method.

在此基础上，首次成功建立了对三唑磷具高特异性的间接竞争、包被抗体直接竞争酶联免疫吸附分析技术。

Via eleven steps we synthesized phosphonate hapten, inhibitor and target antigen.

为此，本论文首先开展了抗体酶催化有机化学反应的研究。

The horseradish peroxidase tagged antibody was prepared by means of modified NaIO4 method and the HRP tagged hapten was prepared by means of mixed anhydride method.

以辣根过氧化物酶采用改良的过碘酸钠法标记抗体、混合酸酐法标记半抗原。

Methods:YCP was coupled to bovine serum albumin to construct three neoglycoprotein s which are different in their degrees of substitution(DSs;≈3,≈6,or ≈10 mol of BSA carrier/mol of YCP hapten,respectively),and their immunogenicities were evaluated in mice following subcutaneous immunization,respectively.

将YCP与牛血清白蛋白共价偶联，获得取代度分别约为3、6和10的3种拟糖蛋白，经皮下分别接种ICR小鼠，测定免疫血清中多糖YCP的特异性抗体IgM和总IgG水平，以及半抗原和载体蛋白的特异性IgG亚型。

It has good specificity and sensitivity by a competitive inhibition enzyme-linked immunosorbent assay. The hapten was linked with enzyme and determination method was established primarily that based on competitive ELISA. The limit of this method was 1ng/ml. The study will be useful for studying the immunoassay in the determination of CL residues.

抗体的亚类为IgM，腹水及细胞上清间接ELISA效价分别为1：20 480和1：320，通过竞争阻断实验证明其具有良好的特异性及敏感性，将半抗原经酶标后应用竞争ELISA方法初步建立了CL的检测方法，检测限可达1ng/mL，为进一步制备CL残留检测试剂盒打下了基础。

Both kinds of immunogen can successfully elicit 36AA specific antibody response in rabbits, which have different immune characteristics. To couple the hapten with vector proteins is a quicker and more effective way to prepare the immunogen.

偶联蛋白方法与基因工程方法制备的免疫原均可成功刺激家兔免疫系统产生针对36肽半抗原的有效抗体，但两者具有较为不同的免疫特性，偶联蛋白方法更加迅速有效。

Using hapten penicillin G as a model, the nanogold in size of 9 nm was used to label rabbit anti-penicillin G antisera to obtain an immunonanogold probe for PG.

以半抗原青霉素G为模型，用粒径为9 nm的金纳米微粒标记羊抗兔青霉素噻唑蛋白抗体制备了青霉素G的免疫纳米金共振散射光谱探针。

Serum titers of antibody to HB surface antigen were measured at 1, 3, and 12 months posttransplantation.

移植后1个月、3个月和12个月分别检测血清中HB表面抗体的滴度。

Purpose: To study the role of P-glycoprotein in chemotherapy resistance of the head and neck malignancies.Method: The expression of P-gp in 50 histological specimens of head and neck cancer were detected by monoclonal antibody MRK 16,in-cluding 38 squamous cell carcinomas, 9 adenocarcinomas. 3 sarcomas.

目的：探讨头颈部恶性肿瘤P-糖蛋白在化疗耐药中的作用、方法：用单克隆抗体MRK16检测50例头颈部恶性肿瘤（鳞癌38例，腺癌9例，肉瘤3例）组织标本中卜糖蛋白的表达。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。