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The construction of the recombinant plasmid carrying shRNA to HPV6bL1 lays the basis for the study of its inhibitive effect on cauliflower excrescence.

经测序,测序结果均与目的序列相同,均符合设计要求。结论 HPV6bL1 shRNA质粒重组体的成功构建为研究尖锐湿疣基因靶向抗体打下基础。

In this study, a recombinant plasmid generating short hairpin RNA was constructed — To construct the recombinant plasmid carrying shRNA to HPV6bL1 and analyze the nucleicacid sequence for further searching new gene therapy method of cauliflower excrescence.

目前对于尖锐湿疣仍无有效的病毒特异性防治措施来保护人类免受其危害,而基因疫苗则为预防HPV感染提供了可能性;L1是HPV6型主要的衣壳蛋白,具有较强的免疫原性,可刺激机体产生保护性的中和抗体

Exogen ous c-fos antibody could decrease the area density ratio of bFGF mRNA to 4.60± 0.86 at the first day post-burn. The bFGF mRNA expression was increased little without significant difference after exogenous treatment with bFGF. Treatment of c-fos antibody could significantly decrease the expression of PCNA. Through th e re-epithelial process was almost completed at 14th d post-burn, the thicknes s of basal cells was thinner than that in controls.

给予外源性c-fos抗体能抑制bFG F mRNA的表达,伤后1 d其mRNA表达的面密度降为4.60±0.86,伤后14 d创面皮肤虽然基本完成了再上皮化过程,但其基底细胞排列成薄层细胞,PCNA数密度明显低于对照组,同时皮肤全层变薄。

Making gfp fusion protein is a convenient method to mark the expression of interesting proteins, but it may interfere the expression of interesting proteins, since gfp is a quite big protein itself.

一方面,作为标记与目的蛋白融合可以方便的标示目的蛋白的表达情况,但gfp本身是个相当大的蛋白,会降低目的蛋白的表达;另一方面,进一步分离时需使用抗gfp的单克隆抗体进行免疫共沉淀,既不经济又不方便。

Results α-Linolenic acid was capable of increasing the quantity of antibody-producing cells and the level of serum hemolysin, promoting the expurgation effect and the phagocytic function of monocyte/macrophage, stimulating...

结果α-亚麻酸能提高小鼠的抗体生成细胞数和血清溶血素水平,促进小鼠的单核-巨噬细胞的碳廓清和腹腔巨噬细胞的吞噬能力,促进小鼠的迟发型变态反应,提高小鼠NK细胞的活性。结论α-亚麻酸具有增强小鼠免疫力作用。

Results There was an markedly efˉfect for astragalus root stimulating the multiplication and transformation of spleen lymphocyte,promoting the delayed type of hypersensitivity,increasing the number of antibody-created cell,enhancing the level of serum hemolysin,promoting the expurgation effect and phagocytosis function of monocyte-macrophage and increasing the activity

结果 黄芪能明显刺激小鼠的脾淋巴细胞增殖、转化作用,促进小鼠迟发型变态反应作用,提高小鼠抗体生成细胞数,提高小鼠的血清溶血素水平,促进小鼠单核-巨噬细胞碳廓清作用,增强小鼠的单核-腹腔巨噬细胞吞噬能力,提高小鼠NK细胞活性。

Results Sheep spleen polypeptides could significantly stimulate multiplication and transformation of mice's spleen lymphocytes; increased markedly the weight of spleen and the thymus; promoted delayed hypersensitivity; increased the quantity of antibody-producing cells; increased the level of serum hemolysin; promoted the expurgation effect and the phagocytic function of monocyte/macrophage and increased the activity of natural killer.

结果 羊脾多肽能使小鼠脾、胸腺增重,明显刺激小鼠的脾淋巴细胞增值,促进小鼠迟发型变态反应作用,提高小鼠抗体生成细胞数,提高小鼠的血清溶血素水平,但促进小鼠单核-巨噬细胞碳廓清作用不明显,增强小鼠的单核-腹腔巨噬细胞吞噬能力,有效提高小鼠自然杀伤细胞活性。

Results There was an markedly efˉfect for astragalus root stimulating the multiplication and transformation of spleen lymphocyte,promoting the delayed type of hypersensitivity,increasing the number of antibody-created cell,enhancing the level of serum hemolysin,promoting the expurgation effect and phagocytosis function of monocyte-macrophage and increasing the acˉtivity of natural killer.

结果 黄芪能明显刺激小鼠的脾淋巴细胞增殖、转化作用,促进小鼠迟发型变态反应作用,提高小鼠抗体生成细胞数,提高小鼠的血清溶血素水平,促进小鼠单核-巨噬细胞碳廓清作用,增强小鼠的单核-腹腔巨噬细胞吞噬能力,提高小鼠NK细胞活性。

Twenty - three patients underwent ictus therapy of corticosteroids for (8±4) days. The extenuation time of corticosteroid' dosage was (33 ±26) mg /d. Of the 40 patients, 36 were IgG positive with an average A value of (0.91±0. 24) and 4 patients were IgG negative. Twelve patients ( 30%) were with type I avascular necrosis of femoral head, including 3 cases with unilateral left - necrosis and 9 cases of bilateral necrosis. The other 28 patients were without necrosis.

结果全疗程使用的糖皮质激素总量平均为4949 mg±2959 mg,糖皮质激素应用天数平均为24 d±5 d,其应用天数主要集中在16-30 d,使用冲击疗法的病例有23例,其持续天数平均为8 d±4 d,减药速度平均为33 mg/d±26 mg/d.40例患者中,血清抗体IgG测定阴性4例,阳性36例,其吸光度值平均为0.91±0.24。

Objective: In order to study the function of T7 promotor in expression of Fab.

目的:探索强启动子T7在可溶性抗体Fab表达中的作用。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

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