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Coli BL21 and purified. The binding activities of immune sera to three EDs were examined with ELISA and Western blotting analysis.②Asthma model was induced with ovalbumin in mice of vaccine group, control vector group and asthma group. Airway pressure-time index and number of cells in BALF were checked. The quantity of goblet cells in bronchiole and the level of MUC5AC mRNA in lung were investigated by PAS staining and RT-PCR assay.

采用ELISA和Western blotting法检测免疫血清抗体对ED的亲合力。2、对DNA疫苗组小鼠制备OVA致敏哮喘模型,检测气道压力峰值—时间指数、支气管/肺泡灌洗液中炎性细胞数量,PAS特染观察小支气管杯状细胞数量及RT-PCR法检测肺组织中粘蛋白MUC5AC mRNA水平,免疫组化和RT-PCR法检测GM-CSF的蛋白与mRNA水平。

Coli were purified and used as the coating antigen in indirect ELISA test, and the results were compared with those obtained by the traditional tube agglutination test method in 100 serum samples in general survey for the Brucella infection.

以大肠杆菌表达、纯化的BP26和OMP31重组蛋白为包被抗原,初步建立了检测血清特异抗体的间接ELISA方法,并以现地牛布氏杆菌普查检测血清100份为样本,与传统的试管凝集试验进行了比较研究。

Objective: To study the application of RBPT and SAT in the detection of Brucellosis.

目的:了解虎红平板凝集试验和试管凝集试验在布病抗体检测中的应用。

Results Thirty-two proteins of Y.pestis were detected in bubonic plague patients.

结果在腺鼠疫患者体内检测到32种蛋白相应的抗体

Calicheamicin can be conjugated to the non-specific antibody using a 4-(4'-acetylphenoxy) butanoic acid or (3-Acetylphenyl) acetic acid linker.

可使用4-(4′-乙酰基苯氧基)丁酸或(3-乙酰基苯基)乙酸连接子使卡奇霉素与所述非特异性抗体共轭结合。

A novel platinum immunosensor modified with hepatitis B surface antibody on colloidal gold and polyvinyl butyral has been fabricated to detect hepatitis B surface antigen.

报道了一种基于聚乙烯醇缩丁醛和金溶胶,把乙型肝炎表面抗体固定在铂金电极上,用来检测乙型肝炎表面抗原的一种新型电位型免疫传感器。

Many antibodies against pro-inflammatory cytokines and inhibitors of inducible NO synthase have been produced, but most of them are agents targeting one factor and cant efficiently block the development of systemic inflammation.

尽管已经研制出许多抗促炎细胞因子抗体及诱生型NO合酶(inducible NO synthase,iNOS)抑制剂等,但多为单因素抗炎制剂,不能有效地阻断全身性炎症的发展。

Type Ⅱ collagen-induced arthritis in mice,which shares similar characteristics with rheumatoid arthritis,was used as the mode for RA in this study,by taking the incidence,arthritis index,the level of anti-collagenase Ⅱ antibody,the histo-pathological changes as quotas,we observed cantharis plaster's effect on the model by applicating it on acupoints,and measured the splenic interleukin-1 β level of collagen-immunized mice.

采用与类风湿性关节炎特征相似的小鼠胶原诱导性关节炎(Collagen-induced arthritis,CIA)模型,以关节炎发病率、关节炎指数、抗Ⅱ型胶原抗体水平、组织病理学改变等为指标,观察了斑蝥膏穴位敷贴对此模型的影响,并检测了胶原免疫小鼠脾脏白介素-1β水平。

By 42 days postvaccination, pigs in group 1 and group 2 had seroconverted to PCV2 capsid antibody.

在免疫后42天,PCV1-2组及真核表达质粒组产生了PCV2抗体

For normal mouse, Yi Shen Granule could prolong the swimming time of mouse, the survival in Yi Shen Granule groups was more than that in model control group in same cold condition. For low-immune mouse, The eliminated rate of carbon granule in Yi Shen Granule groups rats was faster than that in model control group rats, it showed that phagocytosis was boosted up, the absorbency of upside liquid in Yi Shen Granule groups rats was more than that in model control group rats in hemolysin mensurated test, it indicated that more antibody was come into being by giving Yi Shen Granule.

益肾颗粒可以延长小鼠游泳时间;增加在同一寒冷条件下,相同时间内小鼠的存活数;对免疫力低下小鼠,给药组动物的碳粒廓清指数增大,反映吞噬功能增强;在血清溶血素含量测定试验中,给药组动物的上清液吸收值增大,说明机体产生的抗体较多。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。