抗体

Using progesterone and bovine serum albumin as model compounds respectively, fluorescent labeled small molecule hapten and large molecule antigen were prepared.

1以孕酮和牛血清白蛋白为模型化合物，制备了小分子半抗原和大分子抗原的荧光标记物，研究了标记物的抗原活性和光谱性质，结果表明标记物是有效的毛细管电泳免疫分析试剂；制备了抗体F〓片段，研究了其抗体活性，结果表明F〓片段比整个抗体更适合作为毛细管电泳的免疫分析试剂。

Immunohistochemical method: The CD34 monoclonal antibody was selected as the first antibody, with the immunohistochemical SP procedure, then the pathological slice was displayed by DAB and stained again by hematein. One slice was used as a negative control and reacted with PBS fluid(0.01mmol/L) which was the substitute of anti- CD34.(the kits coming from Boster Biotechnology CO.LTD). The number of the vessels and vascular endothelial cells were displayed by immunohistochemical method.

免疫组化方法：病理组织切片以CD34单克隆抗体作为第一抗体，按照免疫组织化学SP步骤进行，DAB显色，苏木素复染，每组用0.01mmol/l的PBS液替代CD34抗体作阴性对照(试剂购自于武汉博士德公司)，应用免疫组化显示血管，对不同时期的血管生成情况及早期的炎性反映情况进行观察。

We coupled the IgG of monoclonal antibody N34 specific to UK and the Fab of monoclonal antibody SZ-51 specific to the membrane protein GMP-140 of activated platelet. The bispecific antibody (N34 SZ-51) is able to bind UK and plasma-clot simutaneously. It enhanced plasma clot lysis 5 fold by HUK whereas it enhanced plasma clot lysis 38 times by LUK. UK in association with the bispecific antibody didn't decrease the concentration of fibrinogen in the assay supernatants.

利用抗体介导使纤溶酶原激活剂定向到血栓表面，可大大提高其纤溶作用，我们用双功能团试剂将抗尿激酶的单克隆抗体N34的IgG和抗活化血小板膜蛋白GMP-l40的单抗SZ-5l的Fab片段通过二硫键共价偶联，合成的双专一性抗体（N34-SZ-51）既具有血栓亲和性也具有尿激酶亲和性，从而增加了尿激酶对血栓的专一性，使高分子量尿激酶溶栓率提高了5倍，使低分子量尿激酶溶栓率提高了38倍之多，且不影响血浆中纤维蛋白原的含量。

It was found that the vaccines used did not cause the adverse reactions such as hypersusceptibility and changes of feed intakes, and that 2 times of vaccinations of the vaccines all effectively enhanced the antibody levels, respectively, in which the No.Ⅲ vaccine stimulated antibody as rapid as the first vaccination was done while the No. I and No.Ⅱvaccines did not result in the antibody by the first vaccination. It was concluded that the No.

结果发现，三种供试的高致病性蓝耳病灭活疫苗均未引起供试猪发生过敏反应和采食量变化等不良反应；三种供试灭活疫苗经2次免疫后，均能提高抗体水平，其中供试疫苗Ⅲ在首次免疫后即能有效产生抗体，而供试疫苗I和供试疫苗Ⅱ在首次免疫后不能有效产生抗体。

A double-antibody sandwich ELISAemploying monoclonal antibody 5G5 was developed for detection of P27 antigen of avian leucosis virus.The limit of viral antigens detected was 5?

利用原核表达的禽白血病病毒P27蛋白作为抗原制备的单抗作为包被抗体，以制的酶标兔抗P27作为酶标抗体，在国内首次建立了检测ALV抗原的双抗体夹心ELISA方法。

Phenotype analysis of transgenic mice Anti-IgM/anti-IgD antibodies, anti-IgM/anti-IgM〓/anti-IgM〓 antibodies were used to analyze allelic exclusion of transgenic mice B cells, and it was shown that 50%～80% B cells express transgene encoded IgM. ELISA was used to evaluate serum antikeratin IgM and total IgM of transgenic mice peripheral blood, and it is found that sera antikeratin IgM level of several lines of transgenic mice is significantly higher than littermate control. However, there is no difference in serum total IgM level between transgenic mice and non-transgenic mice.

三、抗体重链转基因小鼠表型分析以anti-IgM/anti-IgD抗体、anti-IgM/anti-IgM〓/anti-IgM〓抗体分析转基因小鼠B细胞等位基因排斥情况，发现50％～80％的B细胞表达转基因编码的IgM；ELISA分析转基因小鼠外周血中抗角蛋白IgM和总IgM水平，发现部分转基因小鼠的抗角蛋白IgM水平明显高于对照小鼠，而总IgM水平没有差异。

Results: 36 cases with 22 cases of MSAs and MAAs positive (61%), which MSAs positive rate was 40 percent, the most common MSAs was the anti-Jo-1 antibody (30%), MAAs the positive rate was 28%. Anti-Ro-52 antibody for 18 per cent, and with the anti-Jo-1 antibody highly relevant.

结果：36例患者中22例出现MSAs和MAAs阳性(61%)，其中MSAs阳性率为40%，最常见的MSAs是抗Jo-1抗体(30%)，MAAs的阳性率为28%，抗Ro-52抗体占18%，并且与抗Jo-1抗体高度相关。

Some serum samples from ducks immunized with attenuated DPV were assayed with the ELISA kit, and the antibodies were detected first on day 6 or day 9 post-immunization in ducks vaccinated subcutaneously, orally or nasally, and still detectable on day 60 PI.

应用该试剂盒对皮下注射、口服和滴鼻免疫DPV弱毒的20日龄樱桃谷鸭血液中抗DPV特异性IgG抗体效价进行了测定，皮下免疫鸭于第6d，口服和滴鼻免疫鸭于第9d可在血清中检测到DPV特异性抗体，且至第60d时依然可检测到高效价抗体。

The humanization and specificity of chimeric antibody was identified by ELISA and Western bloting, and the tumoricidal activity of the expressed chimeric antibody was detected by tetrazolium salt phenazine methosulfate assay.

采用ELISA和Western bloting法鉴定抗体的人源性和抗体-抗原结合活性，四唑盐/吩嗪硫酸甲酯比色法检测抗体的生物学活性。

At the same time it researches that when the pigling is 45 days of age,the titer of titer of antibody of sow is higher and it still has protection ability for the pigling and after being immunized concentrated vaccine and common vaccine the pigling and after of antibody raises from transitory decline and after 25 days of immunity the titer of antibody can not reach the critical protective value.

同时研究了仔猪在45日龄时母源抗体效价较高，对仔猪仍然具有保护力，用浓缩疫苗和普通疫苗免疫后，抗体效价由短暂下降至上升，免疫25d后抗体效价达不到临界保护值以上。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。