抗体

The isotypes of the five anti-CT mAbs were identified as IgM heavy chain and λ light chain. For the purification of anti-CT mAb, Hitrap IgM Purification Column was used.

其中CT-3A与CT-9A单株抗体经大量生产以Hitrap IgM Purification Column纯化后，经间接型ELISA免疫分析抗体效价在1：15，625。

Cells inosculation was carried out by standard method.The hybridoma supernatants were selected by indirect ELISA.The selected cells were subcloned three times by limiting dilution .Three hybridoma lines against TGEV rMP were obtained , which were designated as 1B3、3C2、4A5. The isotypes of the McAbs were IgG2a、IgG1、IgG1. The three hybridoma lines can secrete antibodies steadily after culturing and saving for a long time.

并以纯化的M蛋白为抗原，免疫BALB/c小鼠，按常规方法进行融合，间接ELISA方法进行筛选，采用有限稀释法，经过三次克隆，最终获得三株抗重组TGEV M蛋白的杂交瘤细胞系(命名为1B3、3C2、4A5)，分泌的单克隆抗体分别为IgG2a、IgG1、IgG1类，三株杂交瘤细胞在体外长期培养和长期冻存时都稳定地分泌单克隆抗体。

Eleven high-titer anti-IFN-γ mAb-producing hybridoma cell lines were obtained and designated Iγ-A8, Iγ-A10, Iγ-B9, Iγ-C6, Iγ-E3, Iγ-F8, Iγ-F9, Iγ-G4, Iγ-G6, Iγ-H6 and Iγ-H9. These isotypes of mAbs were identified as IgG2a heavy chain and κ light chain.

实验成正z选出11株高效价之单株抗体融合瘤细胞株，分别为 Iγ-A8、Iγ-A10、Iγ-B9、Iγ-C6、Iγ-E3、Iγ-F8、Iγ-F9、Iγ-G4、Iγ-G6、Iγ-H6 及 Iγ-H9，经鉴定其单株抗体同种型全属於IgG2a 重链及κ轻链。

Methods PIWIL polypeptide was synthesized and conjugated to Keyhole limpet hemocyanin as an immunogen. Then PIWIL-KLH conjugations were injected into rabbits subcutaneously to produce polyclonal antibodies.

合成特异性PIWIL多肽，以马来酰胺活化匙孔血蓝蛋白作为载体构建多肽免疫原，通过致敏大白兔，制备兔抗人PIWIL多克隆抗体，用亲和层析法纯化抗体。

Methods AGOs polypeptide was synthesized by chemical method, and conjugated to Keyhole limpet hemocyanin as immunogen. Then AGO-KLH conjugations were injected into rabbits subcutaneously to produce polyclonal antibodies.

合成特异性AGO多肽，以马来酰胺活化匙孔血蓝蛋白(keyhole limpet hemocyanin， KLH)作为载体构建多肽免疫原，通过致敏大白兔，制备系列兔抗人AGO多克隆抗体，然后用亲和层析法纯化抗体。

Sera from 18 Chinese haemophiliacs treated with factor Ⅷ produced in USA from 1982 to 1984 were tested for antibody to LAV/ H T L V-Ⅲ by ELISA. Four patients ( 22.2%) showed strong positive results which were confirmed by immunofluorescence test and Western blot,but none of them has developed symptoms related to AIDS.

对浙江省1982～1984年注射了美国产浓缩Ⅶ因子制剂的18例血友病患者，用酶联免疫吸附法检测了血清中淋巴腺病病毒/人T细胞Ⅲ型病毒抗体，发现4例阳性，并经免疫荧光试验和Western印迹法证实。2例应用了国产浓缩Ⅷ因子者抗体阴性。

"Yes." First Affiliated Hospital of Henan College of Traditional Chinese Medicine Obstetrics and Gynecology of a chief physician, said Wang Lina, if both husband and wife blood group incompatibility, the fetus may be obtained from the genetic father of the missing mother of blood group antigen, blood group antigens of red blood cells that once the mother, mother will produce antibodies, which antibodies can enter the fetus via the placenta, the baby will cause the destruction of red blood cell hemolysis, manifested as jaundice, anemia and other serious symptoms may lead to cerebral palsy; If severe intrauterine hemolytic disease will result in miscarriage or stillbirth.

&是的。&河南中医学院一附院妇产科主任医师王丽娜说，如果夫妻双方血型不合，胎儿就可能从父亲身上遗传获得母亲所缺少的血型抗原，这种血型抗原的红细胞一旦进入母体，母体会产生抗体，而这种抗体可经胎盘进入胎儿体内，就会引起宝宝的红细胞被破坏而溶血，表现为黄疸、贫血等，症状严重可能导致脑瘫；如果在宫内出现严重的溶血症，还会导致流产或死胎。

Mouse monoclonal antibodies against salicylic acid was produced and characterized for the first time. The McAb S2-8F, were derived from an immunogen in which 5-aminosalicylic acid (5-ASA) was conjugated to KLH (hemocyanin from keyhole limpets) through its -NH〓 group. The antibody showed high specificity to SA and even more high specificity to 5-ASA (the cross-reaction is 144. 8％), but very little cross-reactivity with 5-sulfosalicylic acid (0.9％) and 4-aminosalicylic acid (0.3％). Other compounds structurally similar to SA, such as 4-hydroxybenzoic acid, 4-aminobenzoic acid and salicylaldoxime, were not found to exhibit any cross-reactivity.

以5-氨基水杨酸(5-aminosalicylic acid，5-ASA)的5位氨基为偶联位点，血蓝蛋白(hemocyanin from keyhole limpets，KLH)为载体合成了SA-NH-CH〓-NH-KLH，以此为免疫原免疫小鼠，利用杂交瘤融合技术得到三株可产生针对SA抗体的细胞株，并对其中编号为S2-8F的细胞株产生的单克隆抗体的特性进行了研究，发现它对SA和5-ASA具有高亲和力。

As low-leveled antibody were discovered among crowds of people, 226 unit of swine serum were collected to investigate the origin of infection, among which 2 unit were positive .

由于在人群中发现有低水平抗体存在，为了搞清这种感染的来源，2000年3月于保定肉联厂采集猪血清226份，有2份阳性，抗体阳性率为0.88％，GMT为113.14（≥1∶80）。

These results suggest that macaques, rats, marmots, fish and toads could be infected with HEV, and the highest prevalence rate of anti-HEY was found in rats.

猕猴、鼠、旱獭、鱼和蟾蜍中存在HEV感染，鼠抗－HEV抗体阳性率最高；其次为蟾蛛和鱼；鸡和鸽子中未检测到抗－HEV抗体。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。