抗体

Methods The serum of 187 children with pneumonia from July 2002 to December 2004 in hospital were investigated for respiratory syncytial virus, adenovirus 3 influenza viruses A and B, parainfluenza virusestype 1,2,3,4 and coxsackievirus A 1.7, echovirus 7 by employing the indirect immunofluorescence assayfor the identification of nearly 8 different viruses, and 3 different enteroviruses.

采集2002年7月~2004年12月因肺炎住我院儿科的187例患儿血清，应用间接免疫荧光法检测8种常见呼吸道病毒病原体和3种肠道病毒的抗体：呼吸道合胞病毒、腺病毒3(ADV3)、流感病毒A和B型、副流感病毒l~4型、柯萨奇病毒A1、7型、埃可病毒7型的特异型IgM抗体。

The extra-articular manifestations present more frequently in the patients with all three positive antibodies.

三种抗体均阳性较抗体均阴性RA者，关节外表现更多，炎症反应更重。

METHODS: The presence and number of recently developed extra-articular manifestations and a weighted EAM score, as well as the levels of serological markers, were compared between 31 patients with RA with histologically proven vasculitis and 50 patients with RA in whom vasculitis could not be documented histologically. The following markers were evaluated: circulating immune complexes, complement components C3 and C4, class-specific rheumatoid factors, antineutrophil cytoplasmic antibodies, antinuclear antibodies, antiendothelial antibodies, circulating intercellular adhesion molecule-1 and -3, circulating vascular cell adhesion molecule and E-selectin, cellular fibronectin, von Willebrand factor antigen, and C reactive protein.

对比组织学上已证实有血管炎的31例 RA 例和组织学上无血管炎的50例 RA 患者间的临床及血清学标记物，这些资料包括最近发生的关节外表现数量、关节外表现加权得分、循环免疫复合物、补体 C3和 C4、类风湿因子（ IgM-RF，IgG-RF，IgA-RF ）、抗中性粒细胞胞浆抗体、抗内皮细胞抗体、循环细胞间粘附分子-1和-3、循环血管内皮粘附分子和 E-选择素、细胞纤连蛋白、假性血友病因子抗原（ von Willebrand factor antigen ）及 C-反应蛋白。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

The agreement provides PDL with milestone payments and royalties on sales of any antibody humanised by either company.

根据协议， PDL 将为雅培全程人源化3个抗体，通过协作使今后任何一方都能人源化雅培公司选择的抗体。

Following that, the hybridoma cells were injected into the peritoneal cavity of BALB/c mouse.

间接ELISA法测定抗体的效价，Western-blot检测抗体的特异性。

The antibodies secreted by two hybridoma cell lines identified by several methods were specific antibodies of rhANG.

经多种方法鉴定获得的二株杂交瘤细胞分泌的抗体为rhANG特异抗体。

However, there were clear differences in antibody levels and isotypes of IgG among 5 strains of mice.

但特异性抗体水平及抗体亚型分布因遗传背景不同而异。

It was suggested that isotypes of human xenoreative natural antibodies against porcine pancrease islet cells were IgG and IgM.

结果表明：人抗猪胰岛细胞天然抗体的同种型是IgG和IgM型抗体。

The prevailing isotypes of IgG associated with the immunization were IgG1 and IgG2a.

所诱导的4种IgG抗体亚型中，IgG1和IgG2a是免疫血清中主要的抗体类型。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。