抗体

In the method, the polyclonal antibodies are obtained by utilizing syntactic quinoxalone antibiotics multi-cluster antigen immunization; the coupling compound of the half antigen of the quinoxalone antibiotics and an egg-white protein OVA is taken as an envelope antigen; the quinoxalone antibiotics is taken as a standard; the indirect competitive enzyme-linked immune detection method for the quinoxalone antibiotics in animal food is established; a rapid and high efficient detection means is provided for the residues of the quinoxalone antibiotics in the animal food; because the polyclonal antibodies are adopted, the cost is low, and the stability and the repeatability are good; the detection limit (IC is smaller than 90) is 0.0126 ng/ml, the median inhibitory dose (IC is smaller than 50) is 0.52 ng/ml, and the detection range (IC is smaller than 20 to 80) is 0.04467 to 14 ng/ml.

本发明利用合成的喹诺酮类抗生素多簇抗原免疫得到多克隆抗体，以喹诺酮类抗生素半抗原与卵清蛋白OVA的偶联物作为包被抗原，以喹诺酮类抗生素为标准品，建立动物性食品中的喹诺酮类抗生素的间接竞争酶联免疫检测方法；为喹诺酮类抗生素在动物性食品中的残留检测提供了快速高效的检测手段，由于采用的是多克隆抗体，费用较低并且稳定性和重复性较好，检测限(IC 90 )为0.0126ng/ml、半数抑制量(IC 50 )为0.54ng/ml和检测范围(IC 20 ～IC 80 )为0.04467～14ng/ml。

The detection is based on the change of electrode potential before and after the antigen-antibody reaction.

蛋白A为抗原和抗体的反应提供了合理的基础，纳米金的存在提高了抗体在电极表面的固定量，多壁碳纳米管促进了电子的传递，从而缩短电极的响应时间。

Methods samples from erythema nodosum patients and normal people were collected, the anca and aeca were detected by indirect immunofluorescence.

目的 探讨血清抗中性粒细胞胞浆抗体及抗血管内皮细胞抗体在结节性红斑发病中的作用和意义。

Objective To discuss the value of detection of serum serum anti-nuotrophil cytoplasmic autoantibodies and anti-vascular endothelial cell antibodies in the diagnosis of erythema nodosum. Methods Samples from erythema nodosum patients and normal people were collected, the ANCA and AECA were detected by indirect immunofluorescence.

目的 探讨血清抗中性粒细胞胞浆抗体及抗血管内皮细胞抗体在结节性红斑发病中的作用和意义方法收集结节性红斑患者和正常人血清，用间接免疫荧光技术检测血清中ANCA和AECA，并分型。

That the four synthetic peptides were able to be efficiently recognized in ELISA by their respective homologous antisera showed they were all antigenic.

结果显示，4个合成多肽均能被相应的抗体识别，具有良好的抗原性；能诱导产生特异性IgG抗体。

Both the autoantibody levels and the functional basophil phenotype remained stable in subjects with active disease (n=16), whereas there was an enhancement in basophil function as subjects evolved into a state of remission (n=6), which appears independent of the presence of autoantibody.

在处于疾病活动期的患者体内，无论是自身抗体水平还是功能性嗜碱性粒细胞表型均保持稳定（16例），而在疾病的缓解阶段（6例），嗜碱性粒细胞的功能增强，这似乎与自身抗体的存在无关。

The results showed that antibody response were observed in 19 (27.1%) of the 70 children with pneumonia. Excluding one case of cross-reaction, the others 18 cases, were diagnosed as Hib infection five caese had no Hib antigen evidence. Their diagnosis were confirmed only by the antibody response.

结果有19/70(27.1%例)肺炎患儿产生Hib OMP抗体反应，除外1例交叉反应，余18例诊断为Hib感染，其中5例未查到Hib抗原，以Hib OMP特异抗体反应得到诊断。

The mean corrected count increment in 20 patients received platelet transfusion many times was 18.2 after crossmatch and 4.7 before crossmatch.

现在常用的抗体检测方法有血小板单克隆特异性抗体固定试验、微柱凝胶试验、简易致敏红细胞血小板血清学试验等。

Draining lymph nodes isolated from the BALB/C mice immunized with rabbit skin collagen Type Ⅲ accord- ing to a rapid immunization protocol, the lympho- cytes were fused with the myeloma cell line NS-1, 3 out of total of 201 growing hybridomas we obtained were producing collagen Type Ⅲ reactive antibodies after 3 limited dilution clones performed.

本实验采用快速免疫的方法用兔皮肤Ⅲ型胶原免疫BLAB/C小鼠，取淋巴结的细胞与小鼠骨髓瘤细胞NS-1融合，240孔中201孔有杂交瘤生长，用ELISA检测有6孔反应阳性。选3孔生长良好，抗体浓度高的细胞进行有效的有限稀释法克隆3次，获3株分泌抗Ⅲ型胶原单克隆抗体的杂交瘤细胞。其中二株属IgG2b，一株为IgG1。

This thesis introduce a sensitive method for detecting the dopping of Erythropoietin.

本文从促红细胞生成素出发，经过动物免疫，多克隆抗体的制备以及不同的检测抗体的制备等一系列过程，初步建立了一种灵敏检测抗原EPO的方法，从而为简洁、经济、快速的体外检测兴奋剂EPO打下一定的基础。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。