抗体

Serum samples from cases with schistosomiasis or other helminth infections were tested. Results The positive rate of MPAIA was 96.7%（116/120） with the sera of S. japonicum -infected cases. No cross reaction was observed with sera of trichinellosis, paragonimiasis or cysticercosis cases.

结果 用磁微粒分离酶联免疫法检测日本血吸虫虫卵抗体，阳性检出率为96.7%（116/120），与旋毛虫、并殖吸虫、嚢尾蚴等其他寄生蠕虫抗体无交叉反应现象，检测试剂4 ℃可保存12个月。

The aim of this study is trying to better illustrate on immunogens of avian pasteurellosis by generating antibody-secreting hybridoma cells and studying on the characters of antibodies produced from these cells.

因此，本研究拟以巴斯德杆菌免疫小鼠制备单株抗体，并利用所产生之12株单株抗体的研究，尝试辨认禽鸟类巴斯德杆菌之重要抗原。

56 cases of bullous pemphigoid were studied on the clinical characters and immunol-blot patterns.It was found that bullous pemphigoid happened mostly among old people in bullous type;Autoantigen BP230,BP230/180 and BP 230/180 combined with minor antigens involved in acute phase of BP patient;While BP180 involved mainly in the remission of the disease.

对56例江浙沪地区类天疱疮患者的临床特征和不同靶抗原的关系进行了探讨，发现类天疱疮多发于老年人，并以大疱性多见；在BP的急性期抗体结合230kD、230/180kD、合并条带靶抗原较常出现(86.11%)；而在疾病的缓解期抗体结合180kD抗原较常出现(65%)；180kD靶抗原并非次要抗原。

Results Of the 78 patients,15 were cytoplasmic ANCA positive, and all of them were recognized proteinase 3 (PR3); 62 were perinuclear ANCA positive, and all of them were recognized myeloperoxidase.

结果 78例中15例胞浆型抗中性粒细胞胞浆抗体阳性，均识别蛋白酶3(PR3)；62例环核型抗中性粒细胞胞浆抗体阳性，均识别髓过氧化物酶。

It was shown that peroral infection of the larvae with mixture of GP37 and SpltMNPV could not obtained the significant difference from that only with virus. The GP37, however, was fed to the cotton leafworm larvae 30h in advance of virus infection, resulting in around 50% reduction in larvae mortality.

GP37抗体结合实验结果显示，用GP37抗体与病毒粒子结合后感染斜纹夜蛾幼虫，幼虫的死亡率与对照组没有显著差异，证实了GP37蛋白不是存在于病毒粒子外膜上的蛋白或者不是受体结合蛋白。

GFP antibodies were used to immunoprecipitate proteins that were subjected to SDS-PAGE and transferred to a polyvinylidene difluoride membrane, and the presence of HAX-1 was detected using specific antibodies.

24小时后，细胞裂解形容准备在&材料和方法&绿色荧光蛋白质的抗体来immunoprecipitate遭到SDS-PAGE和转让给聚氟膜而在场的HAX-1利用特异性抗体检测。

Twenty-four h later, cell lysates were prepared as described under "Materials and Methods." GFP antibodies were used to immunoprecipitate proteins that were subjected to SDS-PAGE and transferred to a polyvinylidene difluoride membrane, and the presence of HAX-1 was detected using specific antibodies.

24小时后，细胞裂解形容准备在&材料和方法&绿色荧光蛋白质的抗体来immunoprecipitate遭到SDS-PAGE和转让给聚氟膜而在场的HAX-1利用特异性抗体检测。

Firstly, the AC conductivity of this complex was detected on the porus film, and optimizing factor was investigated, such as electrode, frequence, doped ions.

二、把纳米金—抗原—抗体复合物作为工作电极的修饰物，以纳米金的聚集状态表达抗原抗体的结合，从而实现增强修饰电极双电层变化的目的。

Objective:(1) To compare the sensitivity, specificity, and positive prognosticative value in the diagnosis of rheumatoid arthritis by examining antifilaggrin antibody, anti-cyclic citrullinated peptide antibody and rheumatoid factor respectively simultaneously.

目的：①检测类风湿关节炎病人血清抗聚角蛋白微丝蛋白抗体、抗环瓜氨酸肽抗体及类风湿因子，比较单独检测和联合检测对RA诊断的敏感性、特异性、阳性预测值。

METHODS: Female BALB/c mice were treated with CCK-8 and keyhole limpet haemocyanin, splenocytes were acquired and incubated in vitro with KLH restimulation, the productions of Th1 cytokines, interferon-γ, interleukin-2 and Th2 cytokines, IL-4, IL-5 in cell culture supernatants were detected by enzyme-linked immunosorbnent assay. The mRNA expressions of IFN-γ, IL-2, IL-4 and IL-5 in splenocytes were detected by reverse transcription-polymerase chain reaction. The levels of Th1 antibody IgG2a and Th2 antibodies IgG1 in serum were also detected by ELISA.

给予BALB/c小鼠钥孔戚血蓝蛋白免疫同时体内给予不同剂量的CCK-8，酶联免疫吸附试验检测其脾细胞培养上清中Th1型细胞因子γ-干扰素、白细胞介素-2（IL-2）和Th2型细胞因子白细胞介素-4（IL-4）、白细胞介素-5（IL-5）水平，逆转录聚合酶链式反应法检测脾细胞中IFN-γ、IL-2、IL-4、IL-5 mRNA表达；ELISA法检测血清中Th1型抗KLH抗体IgG2a和Th2型抗KLH抗体IgG1水平。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。