抗体

AIM To evaluate the clinical significance and feasibility of detecting serological tumor associated angigen CP 2 and anti idiotypic monoclonal antibody 6B 11 Ab 2 among patients with ovanian tumor.

目的 探讨应用卵巢癌抗原 CP2 及卵巢癌抗独特型单克隆抗体 6 B1 1 Ab2 进行血清中相关抗体检测的可行性及临床应用价值。

Objective:To obtain the completely humanized Fab antibody against idiotypic antibody of schistosoma japonieum from large naive phage-display library.

目的：构建大容量天然人源Fab抗体库，筛选全人源日本血吸虫抗独特型抗体并进行初步鉴定。

Such responses can be induced through the induction of antibody-dependent cellular cytotoxicity, promotion of antibody-targeted cross-presentation of tumour antigens, or by triggering of the idiotypic network.

通过诱导抗体依赖性细胞毒性效应、刺激抗体靶向的肿瘤抗原交叉提呈或触发独特型网络，均可诱导该类应答。

In this study, oral tolerance was induced by tube-feeding transgenic mice with 0.5mg/day OVA protein daily, simultaneously sensitized by OVA protein with aluminum hydroxide. Oral-administered OVA protein (0.5mg/day for 5 or 10 consecutive days) resulted in significant lowered airway hypersensitivity as well as reduced inflammative cells infiltration, titer of serum OVA-specific IgE, IL-5 secretion in bronchoalveolar lavage fluid, and IL-4 secretion in splenocyte.

首先，本实验於小鼠中成功诱发了呼吸道过敏、大量OVA特异性IgE抗体生成、及嗜酸性白血球不正常聚集等现象；致敏前，同以连续管餵小鼠OVA抗原(0.5mg/day) 5天或10天后，结果显示皆能缓和呼吸道阻力、减低肺部发炎细胞浸润现象、降低血清中OVA特异性IgE抗体的生成量、也减少肺部冲洗液及脾脏细胞内第二型辅助T细胞(type II helper T cell， Th2)细胞激素的分泌(IL-4、IL-5)。

Methods: The serum specific ingestive and inhalant allergens of 586 cases of urticaria and urticaria papulosa patients were detected by allergen in vitro test kit.

采用过敏原特异性IgE抗体检测试剂盒对586例吸入组及食物组急、慢性荨麻疹及丘疹性荨麻疹患者血清进行过敏原特异性IgE抗体检测。

Methods Keratinocytes were isolated from the foreskins of children, and subjected to primary culture. The cultured keratinocytes were incubated with anti- keratin 16 antibody, or with isotype antibody as control, for various durations.

将抗角蛋白16抗体加入培养的角质形成细胞中，以同种型抗体作对照，研究外皮蛋白和Toll样受体2和4 mRNA表达。

Amiri and colleagues treated mice with a rabbit polyclonal antimouse anti-IgE or with an isotype control antibody and then infected them with S mansoni.

Amiri及其同事用兔抗多克隆抗IgE抗体或同种型对照抗体处理小鼠，然后使其感染曼氏血吸虫。

Induction of isotypic heterostrain antibody (Ab1): BALB/c mice were immunized with C57BL/6 splenocytes to raise antibody (Ab1) between the two difference strains.

抗同种异品系抗体(Ab1)的诱导 C57BL／6小鼠作为移植供体，BALB／c小鼠作为受体。取C57BL／6小鼠的脾细胞免疫BALB／c小鼠，制备抗同种异品系抗体(Ab1)。

Results Leucocytopenia and lymphocytopenia were seen in all the patients. Two patients had slightly decreased peripheral blood T lymphocyte count. Alanine aminotransferase and aspartate transaminase levels increased slightly in 3 patients. No hypoxemia was seen in all the patients. Both SARSIgM and IgG seroconversion occurred earlier in all the patients with the titer increased more than 4fold shortly. Neutralization test was positive in all the patients. SARS coronary virus RNA was detected by polymerase chain reaction in pharyageal swabs only in 1 patient.

结果 确诊的SARS患者白细胞及淋巴细胞呈不同程度降低，下降时间较流行时提前，下降程度较小；2例外周血T细胞亚群计数轻微降低；3例丙氨酸转氨酶和天冬氨酸转氨酶轻度升高；肾功能、血糖、血脂及心肌酶均无异常；所有患者血清SARSIgM/IgG抗体早在发病第6 d由阴转阳，滴度在短期内呈4倍以上升高，中和抗体实验阳性，1例咽拭子中检测出SARS冠状病毒RNA。

Convey albumen immunity with rarefied LTb 8 Zhou Ling BALB/c rat 3 hind, after taking lienal cell and cell of SP2/0 bone myeloma to undertake confluence, undertake filtration with indirect ELISA and finite and attenuant law, obtain 4 individual plant to be able to stabilize the individual plant of cross tumour cell that secretes antibody of Chan Kelong of LTb of bacili of bowel of the Chinese People's Anti-Japanese Military and Political College, antibody of odd clone of this individual plant names for 1F2, 2C2, 3D6, 4H6 individual plant.

用纯化的LTb表达蛋白免疫8周龄BALB/c鼠3次后，取脾细胞和SP2/0骨髓瘤细胞进行融合后，以间接ELISA和有限稀释法进行筛选，获得4株能稳定分泌抗大肠杆菌LTb单克隆抗体的杂交瘤细胞株，该株单克隆抗体命名为1F2，2C2，3D6，4H6株。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。