抗体

To characterize the isotype of human xenoreactive nat ural antibodies against porcine pancrease islet cells, cyostat sections of the p ancrease were incubated with the sera from 18 cases of IDDM or 20 human controls respectively, further with FITC-conjugated goat antihuman IgG or IgM secondary antibodies. IgG was found to bind to pancrease islet cells in sera from 8 cases of IDDM and 8 human controls, while IgM in 4 IDDM and 6 human controls.

为检测人抗猪胰岛细胞天然抗体的同种型，采取18份胰岛素依赖型糖尿病患者血清及20份正常人血清分别与杂种猪胰腺组织的冰冻切片孵化后，再用免疫荧光标记的羊抗人IgG、IgM单克隆抗体染色。8份IDDM血清和8份正常人血清中含有直接针对猪胰岛细胞的IgG型抗体；4份IDDM血清和6份正常人血清中含有IgM 型抗猪胰岛细胞抗体。

Results We found GM1 antibody in 36% and cephalin antibody in 43% of MS patients in serum,GM1 antibody in 11% and cephalin antibody 18% in cerebrospinal fluid.The levels of antibody in serum of treated_MS patients were significantly lower than those in active MS patients.The levels of myelin basic protein in serum and cerebrospinal fluid in MS were significantly higher than those in control group.

结果 多发性硬化患者血清GM1抗体阳性率 36%，脑磷脂抗体为43%；脑脊液GM1抗体为11%，脑磷脂抗体为18%，与对照组比较差异均有显著性；血清和脑脊液的髓鞘碱性蛋白增高亦有意义。

Results We found GM1 antibody in 36%and cephalin antibody in 43%of MS patients in serum,GM1 antibody in 11%and cephalin antibody 18%in cerebrospinal fluid.The levels of antibody in serum of treated_MS patients were significantly lower than those in active MS patients.

结果 多发性硬化患者血清GM1抗体阳性率为36%，脑磷脂抗体为43%；脑脊液GM1抗体为11%，脑磷脂抗体为18%，与对照组比较差异均有显著性；血清和脑脊液的髓鞘碱性蛋白增高亦有意义。

After immunization, kinetics of antibody response, isotype of IgG antibody, and antibody responses to the individual components of the chimeric antigen were detected by ELISA. Interactions of the antibodies with native antigens located on surface of merozoite of Plasmodium falciparum were analyzed by indirect immunofluorescent assay.

以ELISA检测免疫血清中特异性抗体的动态变化、IgG抗体亚型及对融合抗原各组分的抗体水平，以间接荧光抗体实验分析免疫血清对恶性疟原虫天然抗原的识别情况。

By phage display technique, we prepare ouabain ScFv, which could reduce the murine and reserve its affinity of polyclonal antibody.

随后通过噬菌体抗体库制备单链抗体，可大大降低了哇巴因多克隆抗体的鼠源性，而又保留了原抗体的亲和活性，为研制一种新的降压药奠定了基础。

A sandwich ELISA was established taking the antiserum as the primary antibody. The sensitivity of the ELISA was 0.412 ng GH/ml of sample. Inter-assay and intro-assay coefficients of variation were 2. 58％(n=6) and 5. 69％(n=6) respectively. Serial dilutions of serum and pituitary homogenate from orange-spotted grouper yielded dose response curves that were parallel to the standard curve. And the GH of Acanthopagrus butcheri was also parallel to the standard curve. Prolactin of goldfish and recombinant growth hormone of common carp showed no cross-reactivity with the antiserum of orange-spotted grouper GH. It showed that the established RIA was specific and sensitive.

以斜带石斑鱼重组GH抗血清为第一抗体，羊抗兔抗体为第二抗体建立了双抗体夹心酶联免疫测定法，该系统的灵敏度为0.412ng GH／ml(n=6)，组内变异系数为2.58％(n=6)，组间变异系数为5.69％(n=6)，斜带石斑鱼血清和脑垂体稀释液曲线与斜带石斑鱼重组GH蛋白的标准曲线相平行、黑棘鲷GH的系列稀释曲线与标准曲线相平行，金鱼催乳素、重组鲤鱼GH的系列稀释曲线与标准曲线不平行，表明建立的斜带石斑鱼GH ELISA具有良好的特异性和重复性，灵敏度达到了测试血清样品中GH的水平。

Methods VEGF in the serum of 60 patients with RA and 28 healthy control subjects was quantified by our highly sensitive enzyme-linked immunoad sorbent assay, and its relationship to clinical and laboratory variables was analyzed . Synovial specimens were obtained from 1 RA and 1 OA patients undergoing synovectomy.

体内实验：用酶联免疫吸附方法检测了60例RA和28例健康对照血清中的VEGF值，并检测其他活动性指标及血清中特异性自身抗体：抗核周因子抗体、抗角蛋白抗体、抗环瓜氨酸肽抗体，分析它们之间的关系。

Thy-1.1~+ OT-I CD8 T cells were adoptively transferred into Thy-1.2~+ host C57 mice treated with either isotype or PK136 Ab as mentioned above followed by injection i.v.withΔactA LM-OVA.On day 7 after infection,equal number of Thy-1.1~+ CD8 T effector cells from either hosts were sorted and adoptively transferred into second host mice(Thy-1.2) which were also on day 7 after infection and treated with isotype or PK136 Ab,respectively.

为进一步阐明NK细胞促进CD8Tm细胞的产生是作用于收缩期而不是扩增期，我们设计了如下的实验：将Thy-1.1~+ OT-I CD8 T细胞过继回输到经PK136抗体或对照抗体处理的C57小鼠体内并用ΔactA LM-OVA感染，7天后将两组小鼠脾脏中的Thy-1.1~+ CD8T细胞分离纯化后等量分别回输到同步感染的经PK136抗体或对照抗体处理的第二受体小鼠体内。

The results presented here demonstrate that the highly specificantibody-antigen interaction can be used to generate single-moleculemaps of specific types of molecules in a compositionally complexsample while simultaneously carrying out high-resolution topographicimaging.

为了能够在新模型中应用原子力显微镜，他们在原子力显微镜的尖端粘上针对单个蛋白的抗体。为了保证抗体探针是真正敏感的，用一个多聚物把抗体和尖端连接起来，使抗体摆动到与蛋白受体更易结合的位置。

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。