抗体

Of the 8 cases, 2 cases showed blood Tb-Ab positive, 1 case was positive to antiacid bacillus of sputum, the rest cases with negative TBAb. 3 patients with 2 showed organs infection, 6 patients passed the tissue biopsy diagnose, body temperature of 6 cases fall to normal after 3-7 days of antituberculotic treatment, mild liver dysfunction appeared in 2 patients, hyperuricemia occurred in 7 patients, in addition, 1 patient developed reject reaction. Among all the cases, 7 cases healed, 1 died of pulmonary fungal infection.

其中2例血Tb-Ab，1例腹水结核抗体阳性，1例痰抗酸杆菌阳性，其余结核抗体阴性，3例患者表现为2个脏器感染，6例通过病理组织活检诊断，6例发热者在抗结核治疗3~7d体温降至正常，2例患者在治疗过程中出现轻度肝功能异常，7例出现高尿酸血症，1例出现移植肾排斥反应。8例患者7例痊愈，1例肺结核患者死于肺部真菌感染。6例发热者在抗结核治疗3~7d体温降至正常。

Diagnostic performances of anticyclic citrullinated peptides antibody and antifilaggrin antibody in Korean patients with rheumatoid arthritis.

AKA、APF、RF和抗CCP抗体在类风湿关节炎诊断和鉴别诊断中的应用。医学检验与临床 2006， 17(6)： 35-37.56 潘解萍，谢雯。抗环瓜氨酸肽抗体在类风湿关节炎早期诊断中的临床意义。

The role of anticyclic citrullinated peptide antibodies in the differential diagnosis of elderly-onset rheumatoid arthritis and Polymyalgia rheumatica.

中华临床医学研究杂志， 2007， 13(16)： 2420-2421.30 王永福，康丽荣。联合检测抗环瓜氨酸肽抗体及抗角蛋白抗体在类风湿关节炎患者中的临床意义。

Anti-GD1a antibodies were highly associated acute motor axonal neuropathy while high titers of anti-GM1 were more frequent indicating that GD1a possibly targets the axolemma and nodes of Ranvier [3] most of the Ab+ patients had C.

反GD1a抗体得到高度相关的急性运动性轴索神经病，而高滴度的抗神经节苷脂被更加频繁，表明GD1a可能目标在axolemma和节点的郎飞[ 3 ]大多数的抗体+病人角 jejuni infections。

Aim: to develop monoclonal antibody against benzylpenicillin and to establish a sandwich elisa method for relative quantitative analysis of the allergen which induced penicillin allergy commonly in clinic.

目的： 制备抗青霉素的单克隆抗体并建立双抗体夹心elisa检测方法，对临床上引起青霉素过敏反应的过敏原青霉噻唑蛋白进行研究。

According to the result, we believed that the reason resulted in the low sensitivity was that the marker residue compound MQCA structure has changed after conjugated with protein and the antibody induced by the conjugation could not recognize the free MQCA in the ci-ELISA. Therefore we designed some hapten which could reserve the marker residue structural speciality mostly. After synthesis route selection and optimum, the hapten 7-Hydroxymethyl-3-methyl-quinoxaline-2-carboxylic acid was synthesized, used 4-methyl-2-nitroaniline as the start material via six steps, such as oxidation cyclization, bromize reaction, hydrolysis, Beirut reaction, reduction, hydrolysis. This work established basis for producing the high affinity antibody and developing the sensitive ELISA residue detection method.

本论文认为导致ELISA检测灵敏度较低的原因，是残留标示物和蛋白偶联后的特征结构发生改变，诱导产生的抗体不能够对游离MQCA很好的识别，因此，本论文设计了能够最大化保留MQCA结构特征的系列半抗原结构，并对其合成路线进行了筛选和优化，最终以对甲基邻硝基苯胺为起始原料，经氧化环化、溴代、水解、Beirut缩合、还原、水解六步反应制备含羟甲基结构的MQCA衍生物7-羟基甲基-3-甲基-喹噁啉-2-羧酸，为制备对游离残留标示物高亲和力的抗体，高灵敏度的ELISA残留检测方法的建立奠定基础。

Methods: Agar gel immunodiffusion test was conducted twice in agar gel plate with 0.1 mg/L bromophenol blue, first, agar gel immunodiffusion test was carried out with AFP-HSP70 cross-linking protein and AFP antibody, then the test was conducted again with the processed precipitation band and HSP70 antibody.

在含0.1 mg/L溴酚蓝的凝胶琼脂板上，进行2次琼脂免疫扩散实验，第1次是用AFP-HSP70交联蛋白和AFP抗体进行琼脂免疫扩散，把扩散后沉淀带处理后再次和HSP70抗体进行琼脂免疫扩散。

The results indicated that the purity and immunoactivity varied according to different purification methods. IgG purified by caprylic acid precipitation plus ammonium sulfate precipitation is the best for further use with 98.05%purity and 1800 ratio activity.

结果表明，不同方法纯化的抗体其纯度和免疫学活性具有一定程度的差别，其中经辛酸+饱和硫酸铵沉淀纯化的抗体为最佳，凝胶扫描纯度为98.05%，比活性近1 800，为纯化前的6.8倍。

The main results are as follows: 1 Development of colloidal gold immunochromatographic assay for rapid detection of AFB_1 (1) One ascitic IgG monoclonal antibody was purificated by caprylic acid-ammonium sulfate precipitation.Activities and purity of monoclonal antibody were monitored by indirect ELISA and SDS-PAGE, respectively.

本论文研究的内容主要包括以下两个方面： 1 AFB_1胶体金免疫层析快速检测方法的研究(1)利用辛酸-硫酸铵盐析法纯化小鼠腹水中抗AFB_1单克隆抗体，分别采取间接非竞争ELISA和SDS-PAGE对纯化后抗体的活性和纯度进行鉴定。

The highest titer of anti-CT mAb (CT-3A and CT-9A) determined by indirect ELISA was 1:15,625. Secondly, we made the colloidal gold from the chloroauric acid (HAuCl4) and labeled anti-CT mAb with the 20 nm colloidal gold.

第二部分进行胶体金合成工作，并成将霍乱毒素单株抗体与20 nm胶体金进行抗体染色，进一步应用於霍乱毒素免疫分析试片之开发上。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。