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They were spindle-shaped, and showed positive reaction to antibodies against vimentin, and negative reaction to antibodies against keratin.

结果: 3种方法获得的细胞形态和生物学行为大致相同;波丝蛋白抗体染色阳性,角蛋白抗体染色阴性。

Results Four hybridoma cell lines stably secreting McAbs against sporozoites were obtained: Easp-3G3 and Easp-5G10 belonging to IgG1, Easp-3H6 belonging to IgG2b, Easp-5H4 belonging to IgG2a. All four McAbs bound with E. acervulina sporozoite protein, but the Easp-5H4 McAb showed cross reactivity with E.

结果 获得4株可稳定分泌单克隆抗体的杂交瘤细胞株,其中Easp-3G3、Easp-5G10为IgG1 类,Easp-3H6为IgG2b 类,Easp-5H4为IgG2a 类。4株单克隆抗体均能与堆型艾美球虫子孢子的抗原蛋白反应,但仅Easp-5H4能与柔嫩艾美球虫子孢子抗原蛋白反应。

Reporter molecules (thiophenol, bipyridine derivates, cyanopyridine derivates) were attached to different immuno-gold colloids, respectively. By means of the specific adsorption between antigen and antibody, a sandwich structure was constructed, in which labelled immuno-gold colloids and antigen covalently bound to substrate specifically capture different antibodies.

以多种不同的标记分子(苯硫酚,联吡啶类分子,氰基吡啶类分子)分别标记多种不同免疫金溶胶,通过抗体抗原之间所具有的特异吸附性,进一步组装"固相抗体-待测抗原-标记免疫金溶胶"多组分三明治复合体系。

The effect of thymus environment on T cell development and tolerance.

在所挑选的这些抗体中,并不是所有对细胞系阳性的抗体对正常的细胞也呈阳性反应,说明培养的细胞可能表达了一些正常细胞不表达的抗原。

The results showed that all of them can bind to Her-2 protein specifically and also have high stability and high titer.

抗体的稳定性和结合特异性进行了鉴定,结果表明它们能够非常特异地与Her-2蛋白结合,抗体的效价高,稳定性好,在临床诊断肿瘤抗原Her-2中具有很大的应用价值。

We found that the titre of epitope specific antibody in the antisera was enhanced with increased copies of epitopes.

研究结果显示抗血清中表位特异性抗体的滴度随着疫苗中表位数目的增加而升高,说明可以利用表位重复来增强表位特异性抗体应答。

Moreover, the formulation composed of a mixture of different microspheres provided an immune response very similar to that of the three-dose of soluble tetanus toxoid.

微球引起的抗体显著高于破伤风类毒素溶液一次免疫的抗体水平,与破伤风类毒素溶液3次免疫的效果相似。

Sera from patients with other parasitic diseases and healthy individuals were used as control. RESULTS: The anti Trichinella antibodies were detected in 15 infected mice as early as 2 weeks after infection,and in 86 5% of 467 patients with trichinosis.

结果:15只小鼠感染旋毛虫后2wk即可检出抗体,467例旋毛虫病患者的抗体阳性率为86.5%,而丝虫病、包虫病患者各45例和健康人血清56份均为阴性。48份并殖吸虫病患者的交叉反应率为16.67%。

Based on the above-mentioned, two kinds of target glycoclusters with different glycoterminus, flexible linkers and different scaffolds were designed, disaccharide glycocluster (including TM1-TM7) and trisaccharide glycocluster (TM8-TM9) respectively, with the purpose of obtaining glycoclusters with high binding affinity to anti-Gal antibody and thus better inhibiting the HAR.

基于上述,针对HAR产生的机制,以人体中天然存在的anti-α-Gal抗体及B细胞表面的相应受体为靶点,以α-Gal抗原末端的二糖Galα1→3Gal和三糖Galα1→3Galβ1-4GluNAc片段为糖基部分,采用柔性的连接臂(氨基保护的2-乙醇胺和3-巯基丙酸)和刚性的芳香骨架及柔性的脂肪链骨架,设计了两类结构新颖的糖簇分子:即氧苷和硫苷键连接的二糖糖簇TM1-TM7)和三糖糖簇(TM8-TM9),目的在于寻找与anti-Gal抗体及B细胞相应受体有较高结合力的糖簇分子,以便有效地抑制HAR反应的发生。

Unconjugated antibodies show significant efficacy in the treatment of solid tumors by monotherapy and combining with chemotherapy or radiotherapy.

单克隆抗体单治疗和联合化疗、放疗在实体瘤治疗中显示出了很好的疗效,抗体与放射性核素结合的放射免疫性治疗也有明显的疗效。

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Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

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