抗体

OBJECTIVE: Our objective was to examine the prevalence of pendrin antibodies in autoimmune thyroid diseases and compare with that of thyroglobulin, thyroperoxidase, TSH receptor, and sodium iodide symporter antibodies.

目的：本研究的目的是检测自身免疫性甲状腺疾病患者中pendrin抗体的存在情况，并与甲状腺球蛋白抗体、甲状腺过氧化物酶抗体、促甲状腺素受体抗体和钠碘转运体抗体进行比较。

To develop standard WNV diagnostic tools, four monoclonal antibodies against WNV envelope protein domain III were produced. The isotype, the reactivity with denatured and native antigens, the affinity and specificity of antibodies have been characterized. Two MAbs (4B3 and 2F5) were used to establish an antigen capture-ELISA detection system.

为了建立有效的WNV检测方法，本研究制备了四株抗WNV囊膜蛋白第三结构域的单克隆抗体，鉴定了抗体的亚型、抗体和变性及非变性抗原结合的强度、抗原和抗体结合的亲和力常数，进行了免疫荧光分析、测定了抗原表位竞争、抗体的中和能力等，并检测了上述单抗与日本乙型脑炎病毒的交叉反应情况。

An antibody binding to IGF-IR and inhibiting the binding of IGF-I and IGF-II to IGF-IR which is characterized in that said antibody is a is ofIgG1 isotype, b shows a ratio of IC50 values of inhibition of the binding of IGF-I to IGF-IR to the inhibition of binding of IG-II to IGF-IR of 1:3 to 3:1, c inhibits for at least 80% at a concentration of 5 nM IGF-IR phospohrylation in a cellular phosphorylation assay using 3T3 cells providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0.5% heat inactivated fetal calf serum when compared to such an assay without said antibody, and d shows no IGF-IR stimulating activity measured as IGF-IR phophorylation at a concentration of 10 M in a cellular phosphorylation assay using 3T3 providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0,5% heat inactivated fetal calf serum when compared to such an assay without said antibody has improved properties in antitumor therapy.

一种已经提高了抗肿瘤治疗的特性的抗体，所述抗体结合IGF-IR并且抑制IGF-I和IGF-II与IGF-IR结合，其特征在于所述抗体a是IgG1同种型，b显示其对IGF-I与IGF-IR结合的抑制的IC 50 值与其对IGF-II与IGF-IR结合的抑制的IC 50 值的比率为1∶3-3∶1，c当与没有所述抗体的这种测定比较时，其在5nM的浓度上，在包含0.5％的热灭活胎牛血清的培养基中，使用3T3细胞的细胞磷酸化测定中，抑制至少80％的IGF-IR磷酸化，所述3T3细胞提供400，000-600，000分子IGF-IR/细胞，和d当与没有所述抗体的这种测定相比，在包含0.5％的热灭活胎牛血清的培养基中，使用3T3的细胞磷酸化测定中，其在10μM的浓度上没有显示作为IGF-IR磷酸化所测量的IGF-IR刺激活性，所述3T3提供400，000-600，000分子IGF-IR/细胞。

objective to study the efficacy of the tr/patoc 1 slide agglutination assay for vaccination new pentavalent whole cell vaccine for leptospirosis.methods using tr/patoc 1 slide agglutination assay to detect the searial of serum specimens of 100 healthy people after vaccination for leptospirosis and comparing with the microsopic agglutination test.results both basic immunizaton and 20 days after the enhanced immunization,their positive seroconversion rates were higher than 90% in tr/patoc 1 slide agglutination assay,they were not signficantly different between slide agglutination assay and mat,the antibody titer was greatly lower in 90 days after basic immunization.conclusion the tr/patoc 1 slide agglutionation assay was one of useful assays in evaluation the lately immune efficency of leptospira vaccination.

目的 采用tr/patoc 1玻片凝集试验评价钩端螺旋体疫苗免疫效果及应有价值。方法采用tr/patoc 1玻片凝集试验检测100名健康人钩端螺旋体疫苗免疫后的血清抗体变化，并与显微镜凝集试验比较。结果无论是基础免疫还是加强免疫20？d后，玻凝试验抗体阳性率高达90%以上，与标准的显微镜凝集试验比较差异无统计学意义，玻片凝集试验检测的抗体持续时间较短，90？d后阳性率已大幅度降低，表明sat检测的抗体是早期抗体。结论 tr/patoc 1玻片凝集试验操作简易，可以作为基层卫生机构监测钩端螺旋体疫苗近期免疫效果的方法之一。

Results The level of antibodies against monocytes (34.94%) were higher than that of the antibodies against lymphocytes (26.67%) in pretransplant patients. The level of antibodies in first time transplant patients (32.5%) was lower than that of antibodies in retransplant patients (59.7%). The patients with intensity of antibodies against monocytes PRA more than ≥50% only accounted for 1.87%, 10%＜ MPRA ＜50% for 17.62% and MPRA ＜10% 15.44%,respectively.

结果 肾移植术前患者的抗单核细胞抗体水平较抗淋巴细胞抗体水平高，其百分比分别为34.94%和26.67%；而从患者初次和再次肾移植前的比较中可见，初次比再次产生的抗单核细胞抗体水平要低，其百分比分别为32.05%和59.7%；对于抗单核细胞抗体群体反应性抗体的强度，MPRA≥50%的只占1.87%，而50%＜MPRA＞10%以及MPRA＜10%的分别占17.62%和15.44%。

Results The level of antibodies against monocytes (34.94%) were higher than that of the antibodies against lymphocytes (26.67%) in pretransplant patients. The level of antibodies in first time transplant patients (32.5%) was lower than that of antibodies in retransplant patients (59.7%). The patients with intensity of antibodies against monocytes PRA more than ≥50%only accounted for 1.87%, 10%MPRA50%for 17.62%and MPRA10%15.44%,respectively.Conclusion Before renal transplantation, the detection of MPRA level could predict the occurrence of rejection in renal transplantation.

结果 肾移植术前患者的抗单核细胞抗体水平较抗淋巴细胞抗体水平高，其百分比分别为34.94%和26.67%；而从患者初次和再次肾移植前的比较中可见，初次比再次产生的抗单核细胞抗体水平要低，其百分比分别为32.05%和59.7%；对于抗单核细胞抗体群体反应性抗体的强度，MPRA≥50%的只占1.87%，而50%10%以及MPRA结论在肾移植术前检测患者MPRA水平，对肾移植后的排斥反应能起到预测目的。

The minimal ventricular rates ranged from 46 to 80 beats per minute. VVJ mode pacemakers were inserted through xyphoid approach in all live-birth infants. Complications were noted in three of them (50%). Antinuclear antibody and anti-SSA/Ro antibody were positive in all 8 mothers (100%). The anti-SSB/La antibody was positive in 6 of the eight mothers (75%). Five infants tested positive for anti-SSA/Ro antibody.

所有活产婴孩均经由剑突下途迳植入心律调节器，3个(50%)有并发症。8个母亲(100%)有阳性抗核抗体及anti-SSA/Ro抗体，6个母亲有阳性anti-SSB/La抗体。5个婴孩有阳性anti-SSA/Ro抗体，所有婴孩均没有anti-SSB/La抗体。

We find that the changing laws of antibodies in egg yolk and serum of laying hen are almost consistent, and the antibody levei in egg yok are always (more than 15 days after first immunization) high than that in serum. When several bacterins are used simultaneously, they can work in cooperation with each other.

并对这些抗体的动态变化规律进行观测，发现鸡血清抗体IgG与卵黄抗体动态变化规律基本一致，且蛋黄抗体IgY效价在基础免疫15天后总是略高于同期鸡血清抗体IgG；另外发现多株菌在同时免疫时具有协同免疫作用。

We find that the changing laws of antibodies in egg yolk and serum of laying hen are almost consistent, and the antibody Ievei in egg yok are always (more than 15 days after first immunization) high than that in serum. When several bacterins are used simultaneously, they can work in cooperation with each other. The quadratic rotary combination design is used to study the isolation of water-soluble proteins from the Egg Yolk by simple dilution with water.

并对这些抗体的动态变化规律进行观测，发现鸡血清抗体IgG与卵黄抗体动态变化规律基本一致，且蛋黄抗体IgY效价在基础免疫15天后总是略高于同期鸡血清抗体IgG；另外发现多株菌在同时免疫时具有协同免疫作用。

PART Ⅱ IMMUNOPATHOLOGICAL EVIDANCE OF SIALIC ACID STRUCTURE IN CAMPYLOBACTER JEJUNI AS THE CRITICAL ANTIGEN TO INDUCE PERIPHERAL NEUROPATHY To demonstrate the critical role of NANA structure in the pathogenesis of allergic peripheral neuropathy induced by Campylobacter jejuni and provide immunopathological evidence to confirm the supposition of molecular mimicry and cross-immunity between CJ LPS and gangliosides in nerve.

LPS免疫后第2周实验豚鼠的免疫血清中，抗LPS IgG抗体滴度均明显增高，第3周达高峰，第5周仍维持在峰水平，野生株和突变株LPS免疫血清中的抗-野生株LPS IgG抗体滴度较各自免疫前分别增高8倍和4倍，抗-突变株LPS IgG抗体滴度则较各自免疫前分别增高6.5倍和15倍；(2)全身免疫后第3周和第5周，野生株LPS免疫血清中检测到较免疫前增高6倍的抗-GM1 IgG抗体，而NANA缺失的突变株LPS免疫血清中一直未检测到抗-GM1 IgG抗体；(3)野生株LPS免疫组中有17.3％的坐骨神经原纤维发生以轴索变性为主（占65％）的免疫性损伤，与突变株LPS免疫组和对照组比较均有显著性差异。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。