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The four most common phenotypes are A,B,AB,and O,referring to the type of antigen is presentor absenton the surface of a client's red blood cells,Routine testing usually involves only the Rh antigen.if an Rh-negative clinet receives Rh-positive blood,he or she will develop Rh antibodies,and future Rh-positive transfusions may cause a transfusion reaction .in pregnancy,antibodies from an Rh-negative mother may hemolyze fetal(erythroblastosis fetalis,or hemolytic disease of the newborn).

四种最普遍的表现型是A、B、AB、阿,这类型的抗原或表面上的一个客户的红血球、常规测试通常涉及到只有Rhantigen.if准备一Rh-positive接受血液,跟他或她将发展Rh抗体和未来的Rh-positive输血可能引起输血反应,在怀孕期间的母亲可能会产生抗体hemolyze北京(erythroblastosis fetalis或胎儿的新生儿疾病溶血试验确定具体)。

Results : The purity of the antibody was estimated to be greater than 90% after euglobulin precipitation combined with gel filtration, but less than 60% after ammonium sulfate precipitation combined with gel filtration.

腹水含有大量的杂蛋白,采用一步纯化法,不可能得到高纯度的IgM抗体,而多步骤纯化因耗时长,抗体活性损失大。

All of above testify it is the expeditious way to get higher affinity scFv from in vitro immunized antibody library.

这一结果表明取材于体外免疫的淋巴细胞构建免疫抗体库是获得高亲和力抗体的捷径。

In all positive cases, 130 times of positive virus infection were detected, FluB infection in 46 cases(35.38%),FluA infection in 37 cases(28.46%),ADV infection in 21 cases(16.15%),PIV infection in 19 cases(14.61%) and RSV infection in 13 cases(10.00%).

结果 178例COPD患者病毒IgM抗体阳性 75例,为 4 2 13%,在阳性标本中,共有 130例次病毒IgM抗体阳性,其中, FluB 4 6例次,占35 38%; FluA 37例次,占 2 8 4 6

Antibody detection: The chondrocytes of the chinchillas were lysed freezingly with lysis antigen as the mixed antigen, then acted with serum of recipient, and the results showed that no corresponding antibody generated.

抗体检测:将青紫蓝兔的软骨细胞用裂解液冻融裂解作为混合抗原,与受者新西兰兔血清作用,显示相应无抗体产生。

Results Both GAMP-ADH and GAMP-ADH-Pep10 showed GAMP antigenic specificity. The serum IgG level against GAMP induced by GAMP-ADH-Pep10 conjugate was significantly higher than that by GAMP and showed no significant difference with that by GAMP-ADH-TT vaccine. The conjugate also induced immunological memory.

结果 所制备的多糖衍生物GAMP-ADH及多糖-蛋白结合物GAMP-ADH-Pep10均具有GAMP抗原特异活性,免疫小鼠后可诱生比多糖单独免疫更高水平的GAMP血清IgG抗体,且接近GAMP-ADH-TT疫苗诱生的抗体水平,并能形成免疫记忆。

Methods Activate GAMP with cyanogens bromide and prepare GAMP-protein conjugates using ADH as a linker and EDAC as a coupling agent.

方法采用溴化氰活化多糖,以无水己二酸二肼为连接剂,1-乙基-1-3-(3-二甲基氨基-丙基)-碳化二亚胺为偶联剂,制备结合物并免疫小鼠,检测小鼠血清中抗GAMP与抗载体蛋白抗体及抗GAMP抗体的杀菌活性。

Methods Activate GAMP with cyanogens bromide and prepare GAMP-protein conjugates using ADH as a linker and EDAC as a coupling agent. Immunize mice with the conjugates and determine the antibodies against GAMP and carrier proteins in their sera as well as the bactericidal activity of antibody against GAMP.

采用溴化氰活化多糖,以无水己二酸二肼为连接剂,1-乙基-1-3-(3-二甲基氨基-丙基)-碳化二亚胺为偶联剂,制备结合物并免疫小鼠,检测小鼠血清中抗GAMP与抗载体蛋白抗体及抗GAMP抗体的杀菌活性。

The geometric mean titers of ELISA antibody and NA were 3.8 EU/ml and 5.7 IU/ml, respectively.

ELISA检测的抗体和中和试验检测的抗体平均滴度分别为3.8EU/ml和5.7IU/ml。

In 18 cases of MS with blood collected before disease onset, serum geometric mean titers of antibodies to EBV but not CMV were higher than in controls.

有18名MS患者的血液标本,在发病之前被采集,其血清抗EBV抗体滴度的几何平均值,高于对照组,而抗巨细胞病毒的抗体滴度,在两组之间没有差别。

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