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After careful studying their relative importance to immune response and the possibility of the match, seventeen sequences of interest were selected for further experiment, including ESTss analogous to 11.5KD antibacterial peptide, lysozyme, serine protease and its inhibitor, lectin, antifreeze protein, et al. Primers designed according to the sequences were used to amplify the corresponding ESTss from both blood and cephalothorax cDNA library.

在仔细分析了它们在免疫系统中的重要性和在对虾中出现的可能性之后,从中选出了17条可能编码抗菌肽,溶菌酶,凝集素、丝氨酸蛋白酶及其抑制剂,抗冻蛋白等蛋白质的序列,以此为依据设计引物,在中国对虾的血液和头胸部cDNA文库中扩增相应的序列。

Myriocin is a potent inhibitor of sphingosine biosynthesis by blocking the first enzyme in the pathway, serine palmitoyltransferase.

嗜热菌杀酵母素是一种有效的鞘氨醇生物合成抑制剂,能阻止合成途径中第一个酶——丝氨酸软脂酰转移酶。

Methods: The centrifugalization and membrane filtration were used to get rid of the components restraining bacterial from Huangtengsu tablet.

采用离心法薄膜过滤法弃除黄藤素片中的抑菌成分。

Methods: The centrifugalization and membrane filtration were used to get rid of the components restraining bacterial from Huangtengsu tablet.

采用离心法薄膜过淋法弃除黄藤素片中的抑菌成分。

C, 200rpm for 5d then placed statically for more than 7d, and addition more than 0.01% Chitosan to Bacillus subtilis suspension provided significant UV protection. The survival spore rate is 90% about 2-2.5 fold better than control for spore exposed to UV radiation for 30 min. Bioassay in against Glomerella cingulata, and the inhibition activity is still 60%.

枯草杆菌於MIY液态培养基中,30°C,200rpm震荡培养五天后静置培养七天以上,以甲壳素添加浓度大於0.01%作为最适宜的紫外保护剂之作用最好,於UVB灯下暴晒30 min,对枯草杆菌孢子的保护效果最好,存活率达90%以上,抗紫外线活性是不添加紫外线保护剂的CK组的2.5-3倍,对檬果炭疽真菌仍有60%的抑菌活性。

When components of fermentation medium were glucose 20 gL^(-1), yeast extract powder 25 gL^(-1), MgSO4.7H2O 0.38 gL^(-1), L-aminothiopropionic acid 1 gL^(-1), Triammonium cetrate 2 gL^(-1), sodium acetate 2.5 gL^(-1) and tween 80 1 mlL^(-1), the production of bacteriocin reached the maximum and the diameter of bacteriostasis circle was 28.97 mm.

当培养基的组成为:葡萄糖20 gL^(-1),酵母浸粉25 gL^(-1),MgSO4.7H2O 0.38 gL^(-1),L-半胱氨酸1 gL^(-1),柠檬酸三铵2 gL^(-1),乙酸钠2.5 gL^(-1),吐温80 1 mlL^(-1)时细菌素的产量最大,抑菌圈直径可达28.97 mm。

Objective] The study aimed to screen the fungicides that had evident antibacterial effect against Ustilaginoidea virens and their best mixed ratio.[Method] The U. virens strains were separated from rice panicle that had severe disease in rice field of Yaan, 6 fungicides and validamycin and diniconazole mixed with different ratio were selected to carry out the indoor toxicity test on mycelia growth of U. virens with mycelium dry weight method, and the interaction of mixed fungicides was evaluated according to the method of Wadley.

[目的]筛选出对稻曲病菌抑菌效果显著的杀菌剂及其最佳混配比例,[方法]从雅安稻田发病严重的稻穗上分离出稻曲病菌株,选择6种杀菌剂以及井冈霉素和烯唑醇不同混配比例,用菌丝干重法对稻曲病菌菌丝的生长进行室内毒力测定,并根据Wadley方法评价混剂的相互作用。

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