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抑菌素

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The classification of bacteriocin,spectrum of action,mode of action and characteristic of plantaricin were briefly introduced in this manuscript.

植物乳杆菌素是植物乳杆菌产生的一类具有抑菌活性的肽类,可以抑制许多革兰氏阳性菌。

Knamycin and Hygromycin were taken as screening reagents for gerbera resistive plantlets, and Cefotaxime and Carbenicillin as bacteriostats. Under different concentrations, their effects on differentiation of adventive buds were different.

以卡那霉素和潮霉素作为非洲菊抗性植株的选择剂,头孢霉素和羧苄青霉素为抑菌剂,分别采取不同浓度,分析其对非洲菊试管苗不定芽分化的影响。

Dihydromyricetin is an important kind of flavonol. Lysozyme is a antiblastic enzymes which are widely used in the area of medicine, food service industry and chemical lines.

摘 要:二氢杨梅素是一种重要的黄酮类物质,溶菌酶是一种广泛应用于医药、食品、化工行业的抑菌酶类。

Result Based on directly regenerated plant of in vitro plant of Xinjiang cotton, the cotton epicotyl treated by pre-culture for 3-5 d was immersed in bacteria for 15 min and co-cultured for 2 d. Cephalothin at 100 mg/L was used to inhibit bacteria after 50 mg/L kan selection. And the combination of cytokinin with relatively high content and auxin with low content was used in its directly budding culture to obtain resistant seedlings.

结果]在新疆棉花离体培养植株直接再生植株基础上,经3~5d预培养处理过的棉花上胚轴,在浸菌15 min共培养2 d后,经50 mg/L kan筛选,用1000 mg/L头孢霉素抑菌,并用相对高含量细胞分裂素和低含量生长素的组合对其进行直接出芽培养,从而获得抗性小苗。

ResultBased on directly regenerated plant of in vitro plant of Xinjiang cotton,the cotton epicotyl treated by pre-culture for 3-5d was immersed in bacteria for 15min and co-cultured for 2d.Cephalothin at 1000mg/L was used to inhibit bacteria after 50mg/L kanselection.And the combination of cytokinin with relatively high content and auxin with lowcontent was used in its directly budding culture to obtain resistant seedlings.

结果在新疆棉花离体培养植株直接再生植株基础上,经3~5d预培养处理过的棉花上胚轴,在浸菌15min共培养2d后,经50mg/Lkan筛选,用1000mg/L头孢霉素抑菌,并用相对高含量细胞分裂素和低含量生长素的组合对其进行直接出芽培养,从而获得抗性小苗。

Transferred the NP-1 gene into C. ellipsoidea by electroporation with high efficiency expression vector pBinUΩNP1. PCR, Southern blot and Northern dot blot analysis of the G418resistant alga strains indicated the stable integration and correct transcription of NP-1 in C. ellipsoidea genome. In vitro antagonistic assays showed the proper expression of defense gene in C. ellipsoidea. Transgenic C. ellipsoidea is antagonistic to gram negative bacteria—Bacillus subtonic, gram positive bacteria—Escherichiacoli and fungi—Fusarium oxysporum to some extent.

以椭圆小球藻完整细胞为受体,采用电激转化法将连接在高效表达载体pBinUΩNP1上的兔防御素NP-1基因导入小球藻,通过PCR、Southern杂交、Northern点杂交对转基因小球藻进行了分子检测,同时通过体外离体抑菌实验检测了兔防御素NP-1基因表达产物的活性,结果表明NP-1基因已稳定整合到小球藻基因组中,并进行了正确转录和表达。

Soy protein isolate films were prepared with vanillin and their antimicrobial activity was studied.

对香兰素和香兰素添加到大豆分离蛋白膜后的抑菌效果进行研究。

The detection method comprises the steps that: conditions used for an Oxford cup method to determine polyxin B are established, standard solution of the polyxin B is used to draw a linear regression curve under the condition of biological detection, and then an Oxford cup is utilized to identify the size of a bacteriostatic ring produced by zymotic fluid at each stage, thereby measuring the content of the polyxin B in the zymotic fluid.

本发明公开了一种多氧霉素B快速定量分析的检测方法,该检测方法包括:建立牛津杯法测定多氧霉素B的条件,并用多氧霉素B的标准溶液在生物检测条件下绘制一条线性回归曲线,然后利用牛津杯鉴定各阶段的发酵液所产生的抑菌圈大小,从而测得多氧霉素B在发酵液中的含量。

METHODS:We recorded the cardiogram by perfusing garlicin injection and garlic preparation to the isolated heart of rabbits by Langendorff method,examined the minimum dose of asystole. RESULTS:The minimum fungistatic concentration of garlicin injection was 3~6.2 times lower than that of garlic preparation.

采用Langendorf法对兔离体心脏分别灌流大蒜素注射液、大蒜注射液、大蒜实验液,分别记录心搏图,求出致心脏停搏的最小剂量,结果:大蒜素注射液的抑菌浓度为大蒜实验液的1/6.2~1/3,而致心脏停搏的最小浓度分别为0.1736u/ml和3.919u/ml。

And Pyricularia grisea Sacc., with EC50 values of 3.74 and 1.14 mg/L. Particularly it had excellent activity on Blumeria graminis f. sp. tritic Speer and Sphaerotheca fuliginea Poll. with EC50 values of 0.68 and 0.33 mg/L.

结果表明:大黄素甲醚对黄瓜灰霉病菌和水稻稻瘟病菌菌丝抑菌活性明显,其抑制中浓度(EC50值)分别为3.74、1.14mg/L;对小麦白粉病菌和黄瓜白粉病菌具有优异的活性,EC50值分别为0.68、0.33mg/L。

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