抑菌素

Actinomycin D is the potent inhibitor of RNA synthesis, and is demonstrated to be able to induce Sf-9 cell, K562 cell, aleolar macroages cell CTLL-2 and Jurkat cell apoptosis.

放线菌素D是RNA合成抑制剂，具较强的诱导细胞凋亡的能力，实验证明放线菌素D可诱导Sf-9细胞，人白血病细胞株K562，肺泡巨噬细胞，CTLL-2和Jurkat细胞等凋亡。

This paper reaseched the interaction of Dihydromyricetin and lysozyme by using spectrophotography and antiblastic test to measured the activity of lysozyme indirectly,and compared with the activity of the lysozyme which is not acted by the Dihydromyricetin.

本文通过研究二氢杨梅素与溶菌酶的相互作用，用分光光度法和抑菌试验间接测定溶菌酶活性，并与未被二氢杨梅素处理的溶菌酶活性比较。

Moreover, nincomycins could cause hyphase of Rhizoctonia solani kahn wilt and abnormality. The compared effect of nincomycins with chymic pesticide indicated that nincomycins was much better.

比较宁康霉素与农利灵和速克灵对玉米纹枯病菌的抑菌效果，结果显示宁康霉素的抑菌效果优于两种化学农药。

In the same concentration, the antibacterial activity of total pigment was much more significantly than fucoxanthin. It seemed that there were also other kind of pigments with much stronger antibacterial activity in Laminaria japonica.

相同浓度下，海带总色素的抑菌活性比褐藻黄素的抑菌活性显著，说明海带总色素中还含有比褐藻黄素抑菌性更强的其他色素。

The radical scavenging activity of Laminaria japonica pigments was studied by the DPPH2,2-Diphenyl~(-1-picrylhydrazylassay. The total pigments showed a strong DPPH radical scavenging activity of 65.819% with the concentration of 0.52mg.ml~(-1). The DPPH radical scavenging activity of fucoxanthin was 19.85% with the concentration of 0.27mg.ml~(-1). The antimicrobial activity of pigment in Laminaria japonica was studied by the method of round paper. The results showed that the pigment extracts have activity against Escherichia coli, Pseudomonas aeruginosa, Saricina flava and Saccharomyes cerevisia. The antibacterial activity of total pigments for Saccharomyes cerevisia was the strongest with an inhibition diameter of 18.9 mm; and the antibacterial activity of fucoxanthin for Saricina flava was also much significant with an inhibition diameter of 10.2 mm.

测定了它们对自由基的清除率和对四种细菌的抑菌效果，研究结果表明，总色素和褐藻黄素的浓度与自由基清除率成正比，当总色素浓度为0.52mg.ml~(-1)时，清除率达到65.819%，高于陆生植物黑米色素和紫甘薯色素；当褐藻黄素浓度为0.27mg.ml~(-1)时，自由基清除率达到19.85%，低于紫苏色素、紫甘蓝色素、黑米色素、紫甘薯色素；对大肠杆菌、绿脓杆菌、八叠球菌、金黄葡萄球菌的抑菌实验结果显示，海带总色素对金黄葡萄球菌的抑菌活性最强，抑菌圈直径为18.9mm；褐藻黄素对八叠球菌的抑菌活性相对较强，抑菌圈直径为10.2mm。

Results showed that, pyrolin had obvious action on two species of fungi, and the antifungal effect was positively correlative with concentration. The EC50 was 0.4076 mg/mL and 0.4626 mg/mL respectively after cultured 3 days. The main antifungal period was the fast growth period of fungi, and lead to it lag behind. Observation under SEM revealed that pyrolin caused a series of marked morphological and structural alterations of hyphal. These changes included mutual adhesion of mycelium, the hyphal was irregular in diameter, local denting, some of mycelium was broken, disruption of cell wall, outbreaking of inner cytoplast, and the empty cavity appeared. As a result, pyrolin restrained the growth of these fungi.

结果表明，鹿蹄草素对上述两种真菌均具有明显的抑制作用，抑菌效果与浓度呈正相关，培养3d时的EC50分别为0.4076、0.4626 mg/mL；抑菌时期主要是在真菌的快速生长期，使其快速生长期滞后；扫描电镜显示，鹿蹄草素作用下菌体形态发生异常变化，表现为菌丝体之间相互粘连，菌丝粗细不均匀，细胞壁破裂，局部凹陷，部分菌丝断裂，大量内容物外溢，出现空腔等现象，从而发挥其抑菌作用。

Andres, and its mechanism against rot-causing fungi, the antifungal activity and period of pyrolin against Physalospora piricola and Alternaria alternate was evaluated by using agar dilution method, and ultrastructure changes was observed used by scanning electron microscope.

采用中药抑菌实验方法测定了鹿蹄草素对苹果轮纹病菌和梨链格孢菌的抑菌活性和时期，并在扫描电镜下观察菌体超微结构的变化。

Factors that influence percentage of transformation including time of preculture, dipping and coculture, type and content of antibiotic, day of delayed selection culture, intension of selection were studied. The effective transformation system of "Starkrimson" was developed as follow: Leaves precultured in darkness for three days were dipped into agrobacterium suspension for three minutes, then were cocultured in darkness on MS medium with TDZ0.5mg/L and NAA 0.3mg/L for three days, and then cultured in darkness on MS medium with TDZ0.5mg/L NAA0.3mg/L and Cef 250mg/L.

通过对叶片预培养时间、侵染时间、共培养时间、抑菌素种类和浓度、延时筛选时间、不同选择压等遗传转化影响因素的研究，建立的'新红星'苹果遗传转化体系为：叶片黑暗预培养3天，经农杆菌侵染3分钟，在含TDZ0.5mg/L，NAA0.3mg/L的MS培养基上黑暗共培养3天，然后加250mg/L Cef黑暗维持培养3天，再进行Km12.5mg/L的选择压黑暗培养至抗性愈伤出现；转为光照条件1-2次的继代筛选，获得抗性芽后，进行扩繁。

The inhibitory tests in vitro were performed against Escherichia coli, Salmonella pullorum with filter paper method and tube dilution method. And the diameters of inhibition zone, minimal inhibitory concentrations of levofloxacin combined with polymyxin E were measured.

方法］应用纸片法和试管稀释法进行体外抑菌试验，测定左氧氟沙星与多黏菌素联合对鸡大肠杆菌、鸡白痢沙门氏菌的抑菌圈大小和最小抑菌浓度。

Methods After treated with a specific demethylating agent,Aza and acetylating agent, TSA, the status of 5'CpC island methylation of ING1b gene in HT29 human colon cancer cell line was analyzed using methylation specific polymerase chain reaction,and the level of histone acetylation was analyzed by chromatin immunoprecipitation,and reverse transcription polymerase chain reactionwas used to examine ING1b mRNA expression.

应用特异性DNA甲基转移酶抑制剂5-氮-2'-脱氧胞苷（5-Aza-2'-dc，以下简称Aza）及组蛋白去乙酰化酶抑制剂曲古抑菌素A作用人结肠癌细胞株HT29后，用甲基化特异性PCR检测该ING1b基因核心启动子区域CpG岛甲基化情况，用染色质免疫沉淀检测其乙酰化组蛋白绑定的DNA情况，并用逆转录聚合酶链反应检测ING1bmRNA表达。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！