抑菌测定

The bactericidal characters of anise,Chinese cinnamon,lilac,dark plum,semen myristieae and wrinkled giant hyssop were compared by the filter paper method,and the MIC of their extract were tested by culture plate dilute method.

用滤纸片法比较了八角、肉桂、丁香、乌梅、肉豆蔻、霍香等中药材提取液的抑菌性能，用平板稀释法进行了各提取液的最低抑菌浓度测定等试验。

Methods①The minimum inhibitory concentration and the minimum fungicidal concentration of citral against C.

方法①参照NCCLS M27A方案中的微量液基稀释法测定柠檬醛和氟康唑对原始白念珠菌的最低抑菌浓度和最低杀菌浓度；②采用多步诱导法，观察在含梯度浓度(1～50MIC)柠檬醛培养基上连续转种的白念珠菌MIC的变化情况，以氟康唑作为阳性对照，同时设空白对照和溶媒对照；③将敏感性下降的诱导株在无药培养基上连续转种10次后，检测其MIC值；④测定柠檬醛对氟康唑敏感性下降诱导株的MIC值。

Through fungicidal activity bioassay, the peptide inhibited the growths of epiphyte but not bacteria.

采用抑菌圈法测定活性肽的抑菌活性，结果表明，这种活性肽对病原性真菌有明显的抑制作用，对细菌没有抑制效果。

From the effects of 13 Chinese herbs extract on antimicrobial activities against Fulvia fulva, we have known that Dictamnus dasycarps Turcz. and Fructus Arctii extract were preferably.

本研究运用菌丝生长速率法对13种中药提取物进行了抑菌活性的测定，其中白鲜皮、大力子等提取液抑菌效果较好。

This paper deals with the antimicrobial function of both extracts from bamboo leaves of phyllostachys glauca moclure and Artemisia anomala S.

研究测定了淡竹竹叶、六月霜提取液对细菌的抑制作用，并对两者的抑菌效果进行了比较，结果表明：不同提取液有不同程度的抑制作用，比较而言，竹叶的总体抑菌能力强于六月霜。

Methods:The tube broth methods were used to determin minimal inhibition concentrationof 13 common bacteria strains.Results:Extracts of Evodia rutaecarpaJuss.

采用试管稀释法测定吴茱萸提取物对13种临床常见病原菌的最低抑菌浓度，判定抑菌效果。

Coli and S.aureus , the experiment showed that recombined gene peptide has the antibacterialactivities.Conclusion The recombined gene antibacterial peptide was expressed successfully in the fusional pTYB vetor and the fusion gene peptide CA(1 ~8-MA(l ~12),CAM has the antibacterial activities to E. coli and S.aurens. While how to frame a more efficient expression system should be studied further , and its other biological activities are still to be tested.

本实验通过基因克隆方法拼接重组基因抗菌肽与融合表达载体pTYB12并在大肠杆菌中进行表达成功，抑菌活性的检测结果表明重抗菌肤的克隆表达与活性初步测定中文摘要组基因抗菌肤CA(1一8卜MA(1一12)，cAM〕对大肠杆菌与金黄色葡萄球菌均有一定的抗菌活性，如何进一步提高抗菌肤的表达量和有关重组基因抗菌肤的生物学活性还有待于进一步研究。

Toxicity to Macrophoma musae of 13 fungicides and 3 fungicide combinations was determined in the laboratory. Macrophoma musae was highly sensitive to 70% thiophanate methyl, 70% mancozeb, 10% difenoconazole, and 72% Yijunte.

在室内测定了13种药剂和3种复配剂对香蕉黑星病菌的毒力，结果表明：香蕉黑星病菌对70%甲基托布津、70%代森锰锌、10%苯醚甲环唑和72%抑菌特敏感性较高，且抑菌效果好。

Based on the plant growth rate and inhibition zone methods, the antifungal activity of extracts of every organ of Xanthium sibiricum, the fermentation liquid of endophytic fungi MG4-23, and the extract of mycelium were tested with six kinds of pathogenic fungi and three kinds of pathogenic bacteria respectively. The extract of every organ of X.

方法］采用生长速率法和抑菌圈法，分别以水稻纹枯病菌等6种植物病原真菌和大肠杆菌等3种病原细菌为供试菌种，对苍耳各器官提取液、植物内生真菌MG4-23的发酵液及菌丝体提取液进行抑菌活性测定。

Methods: A randomized, single blind controlled clinical design was used to accomplish the series clinical trials included 123 cases. The troop of gatifloxation injection with sodium chloride included 20 cases in gatifloxation group and 23 cases in ciprofloxation group; The troop of gatifloxation injection with glucose included 21 cases in gatifloxation group and 19 cases in levofloxacin group; The troop of gatifloxation pivoxil included 19 cases in gatifloxation group and 21 cases in ciprofloxation group. The antibacterial activity of clinical isolates were detected by scrips which contain gatifloxation(5ug/L), levofloxacin (5ug/L), ciprofloxation (5ug/L), spafloxation(5ug/L), cefotaxime(30ug/L), penicillin(10U/L) respectively. The MICs of sixkinds of antibiotics were detected by 2-fold agar dilution method. Result: The common data of patients in the two groups of 3 troops were similiar.

采用区组分层均衡随机单盲试验设计，分别完成甲磺酸加替沙星氯化钠注射液组试验药20例及对照药23例、甲磺酸加替沙星葡萄糖注射液组试验药21例及对照药19例、甲磺酸加替沙星片剂组试验药19例及对照药21例共123例感染患者的临床试验；用K-B法测定临床分离致病菌对加替沙星(5ug／L)、左氧氟沙星(5ug／L)、环丙沙星(5ug/L)、司帕沙星(5ug/L)、头孢噻肟(30ug／L)、青霉素(10U／L)纸片的敏感性，再用按美国国家实验室标准委员会(National Committee for Clinical Laboratory Standards，NCCLS)推荐的琼脂平皿对倍稀释法测定临床分离菌株对加替沙星、左氧氟沙星、环丙沙星、司帕沙星、头孢噻肟、青霉素的最低抑菌浓度(Minimum Inhibitory Concentration，MIC)以了解其体外抗菌活性。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。