抑菌测定

Methods Using Vibrio eoralliilyticus, Vibrio tubiashii, Vibrio parahaemolyticus and Vibrio earehariae as the tested bacteria, the inhibition zone and the minimal inhibitory concentration were measured. Results The extracts, extracted with ethyl acetate from the leaves of Nerium indicum Mill., Mangifera indica Linn., Melia azedarach L.

采用纸片法和试管法，以溶珊瑚弧菌、塔氏弧菌、副溶血弧菌、鲨鱼弧菌为供试菌株，测定中草药不同极性萃取部分的抑菌圈和最小抑菌浓度。

Methods Using Vibrio coralliilyticus, Vibrio tubiashii, Vibrio parahaemolyticus and Vibrio carchariae as the tested bacteria, the inhibition zone and the minimal inhibitory concentrationwere measured. Results The extracts, extracted with ethyl acetate from the leaves of Nerium indicum Mill., Mangifera indica Linn., Melia azedarach L.

采用纸片法和试管法，以溶珊瑚弧菌、塔氏弧菌、副溶血弧菌、鲨鱼弧菌为供试菌株，测定中草药不同极性萃取部分的抑菌圈和最小抑菌浓度。

The optimal conditions of the pepsin hydrolysis of frame protein in catfish Claris lareza were investigated using a general-purpose rotation unitized design of the second power of four factors, and the content of soluble peptides was determined in the hydrolytic products. The Optimization of Design Expert 7.1.2 revealed that antibacterial activity of the frame protein hydrolysates was controlled by regulation of four process conditions.

采用四因子二次通用旋转设计，以抑菌率为测定指标，对胃蛋白酶水解革胡子鲶Claris lareza鱼骨蛋白的条件进行了优化，同时测定了酶解产物中可溶性肽的含量，以分析抑菌率与可溶性肽含量之间的相关关系。

The analysis methods for the determination of micro or trace elements in high moisture jellyfish were developed. The fatty acid compositions in difderent parts of fresh jellyfish were determined by GC/MS method. Thirty-five fatty acids were identified, and most of them were found in R. esculentum jellyfish for the first time. Especially, two unusual very long chain polyunsaturated fatty acids that were never detected in the other jellyfish also were determined. Amino acids were abundant in R. esculentum jellyfish, especially containing sulfur amino acids, and could be supplied for human diet. The polysaccharide in umbrella part of jellyfish was composed of glucose, galactose and uronic acid, and its molecular weight was 40,000, but the polysaccharide of the oral arms part consisted of glucose, mannose and glycuronic acid, and its molecular weight was 43,000. Above-mentioned data were never reported. The ethanolic extract of oral arms part of jellyfish were extracted by different polar solvents (petroleum ether, acetic ether, n-butanol), and antibacterial activity was tested to these extracts by four species of terricolous pathogenic bacilli and three species of botanic pathogenic fungi. The result demonstrated that the petroleum ether extract had certain bactericidal activity for two species of pathogenic bacilli, and n-butanol extract had certain inhibited activity on apple rot pathogenic fungus.

建立了 高含水量的海蜇产品中微量、痕量元素成分测定的分析方法；采用 GC/MS 方法测定了新鲜海蜇不同部位的脂肪酸组成，共鉴定出 35 种脂肪酸，其中大多数脂肪酸是首次在海蜇中被检测到，尤其是两种不常见的 C24：5 超长链多不饱和脂肪酸的分析和鉴定在其它水母种属中也从未见报道；海蜇三个部位中氨基酸成分齐全，含量丰富，含硫氨基酸含量较高，可与其它食物蛋白质的氨基酸互补；其中海蜇皮多糖是由葡萄糖、半乳糖和糖醛酸组成，分子量为 40，000，海蜇头多糖是由葡萄糖、甘露糖和糖醛酸组成，分子量为 43，000，以上工作均未见报道；利用石油醚、乙酸乙酯、正丁醇三种不同极性溶剂分别萃取海蜇头乙醇浸提物，用纸碟法和生长速率法分别对四株陆源病原菌和三株植物病原真菌进行了抑菌实验，结果表明海蜇头石油醚提取物和正丁醇提取物具有一定的抑菌活性。

The analysis methods for the determination of microor trace elements in high moisture jellyfish were developed. The fatty acid compositions indifderent parts of fresh jellyfish were determined by GC/MS method. Thirty-five fatty acids wereidentified, and most of them were found in R. esculentum jellyfish for the first time. Especially,two unusual very long chain polyunsaturated fatty acids that were never detected in the otherjellyfish also were determined. Amino acids were abundant in R. esculentum jellyfish, especiallycontaining sulfur amino acids, and could be supplied for human diet. The polysaccharide inumbrella part of jellyfish was composed of glucose, galactose and uronic acid, and its molecularweight was 40,000, but the polysaccharide of the oral arms part consisted of glucose, mannose andglycuronic acid, and its molecular weight was 43,000. Above-mentioned data were never reported.The ethanolic extract of oral arms part of jellyfish were extracted by different polar solvents(petroleum ether, acetic ether, n-butanol), and antibacterial activity was tested to these extracts byfour species of terricolous pathogenic bacilli and three species of botanic pathogenic fungi. Theresult demonstrated that the petroleum ether extract had certain bactericidal activity for twospecies of pathogenic bacilli, and n-butanol extract had certain inhibited activity on apple rotpathogenic fungus.

建立了高含水量的海蜇产品中微量、痕量元素成分测定的分析方法；采用 GC/MS 方法测定了新鲜海蜇不同部位的脂肪酸组成，共鉴定出 35 种脂肪酸，其中大多数脂肪酸是首次在海蜇中被检测到，尤其是两种不常见的 C24：5 超长链多不饱和脂肪酸的分析和鉴定在其它水母种属中也从未见报道；海蜇三个部位中氨基酸成分齐全，含量丰富，含硫氨基酸含量较高，可与其它食物蛋白质的氨基酸互补；其中海蜇皮多糖是由葡萄糖、半乳糖和糖醛酸组成，分子量为 40，000，海蜇头多糖是由葡萄糖、甘露糖和糖醛酸组成，分子量为 43，000，以上工作均未见报道；利用石油醚、乙酸乙酯、正丁醇三种不同极性溶剂分别萃取海蜇头乙醇浸提物，用纸碟法和生长速率法分别对四株陆源病原菌和三株植物病原真菌进行了抑菌实验，结果表明海蜇头石油醚提取物和正丁醇提取物具有一定的抑菌活性。

The anti-microbial activity of active materials against valsa mail,fusarium oxysporum,coniothyrium diplodieua and aspergillus nidulans were measured in vitro.the results showed that active materials extracted using acetone,aether and petroleum ether presented fungistasis to different extent on the four pathogenic fungi and had the best effect to coniothyrium diplodieua.active material extracted using ethanol presented certain anti-microbial effect to fusarium oxysporum.the results also showed there were anti-microbial active material in the leaves of cichorium intybus and founded a base on the research and application.

然后以提取液为供试药剂，对苹果腐烂病菌、棉花枯萎病菌、葡萄白腐病菌、构巢曲霉病菌4种植物病原真菌进行离体活性测定。结果表明：丙酮、乙醚、石油醚的提取液对4种病菌均有不同程度的抑制作用，其中对葡萄白腐病菌抑制效果最好；乙醇的提取液对枯萎病菌具有一定的抑制作用。表明菊苣叶中存在抑菌活性成分，补充了菊苣抑菌活性的研究内容，为该种植物抑菌活性成分的研究和应用奠定基础。

The minimum inhibitory concentration of the eight fishery antibacterial drugs are determined and its inhibitory action and bactericidal effects on the Aeromonas hydrophila used respectively and combinedly are compared by using double dilution method and inhibition zone method.

采用二倍稀释法及抑菌圈法测定了目前常用的8种抗菌渔药对嗜水气单胞菌的最低抑菌浓度，比较了这8种抗菌渔药单独用药与联合用药对嗜水气单胞菌的抗菌效果。

Staphylococcus aureus, Streptococcus agalactiae, Bacillus cereus, Escherichia coli, Salmonella choleraesuis, Salmonella enteritidis and Salmonella typimurium were used as tested bacteria, the bacteriostasis was studied and the minimal inhibitory concentration was measured.

以金黄色葡萄球菌、无乳链球菌、蜡样芽孢杆菌、大肠杆菌、猪霍乱沙门氏菌、肠炎沙门氏菌及鼠伤寒沙门氏菌为供试菌，研究其抑菌活性，并测定其相应最小抑菌浓度。

Andres, and its mechanism against rot-causing fungi, the antifungal activity and period of pyrolin against Physalospora piricola and Alternaria alternate was evaluated by using agar dilution method, and ultrastructure changes was observed used by scanning electron microscope.

采用中药抑菌实验方法测定了鹿蹄草素对苹果轮纹病菌和梨链格孢菌的抑菌活性和时期，并在扫描电镜下观察菌体超微结构的变化。

This thesis studied on the antimicrobial activity of clove oil and modified the antimicrobial component-eugenol by structural polishes. The antimicrobial activity of the derivatives of eugenol was determined.

本论文对丁香油中的抑菌活性成分进行了研究，并对得到的抑菌活性成分-丁香酚进行了结构修饰改造，测定了各结构修饰产物的抑菌活性。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。