抑素的

C, 200rpm for 5d then placed statically for more than 7d, and addition more than 0.01% Chitosan to Bacillus subtilis suspension provided significant UV protection. The survival spore rate is 90% about 2-2.5 fold better than control for spore exposed to UV radiation for 30 min. Bioassay in against Glomerella cingulata, and the inhibition activity is still 60%.

枯草杆菌於MIY液态培养基中，30°C，200rpm震荡培养五天后静置培养七天以上，以甲壳素添加浓度大於0.01％作为最适宜的紫外保护剂之作用最好，於UVB灯下暴晒30 min，对枯草杆菌孢子的保护效果最好，存活率达90％以上，抗紫外线活性是不添加紫外线保护剂的CK组的2.5-3倍，对檬果炭疽真菌仍有60%的抑菌活性。

Factors that influence percentage of transformation including time of preculture, dipping and coculture, type and content of antibiotic, day of delayed selection culture, intension of selection were studied. The effective transformation system of "Starkrimson" was developed as follow: Leaves precultured in darkness for three days were dipped into agrobacterium suspension for three minutes, then were cocultured in darkness on MS medium with TDZ0.5mg/L and NAA 0.3mg/L for three days, and then cultured in darkness on MS medium with TDZ0.5mg/L NAA0.3mg/L and Cef 250mg/L.

通过对叶片预培养时间、侵染时间、共培养时间、抑菌素种类和浓度、延时筛选时间、不同选择压等遗传转化影响因素的研究，建立的'新红星'苹果遗传转化体系为：叶片黑暗预培养3天，经农杆菌侵染3分钟，在含TDZ0.5mg/L，NAA0.3mg/L的MS培养基上黑暗共培养3天，然后加250mg/L Cef黑暗维持培养3天，再进行Km12.5mg/L的选择压黑暗培养至抗性愈伤出现；转为光照条件1-2次的继代筛选，获得抗性芽后，进行扩繁。

Previous studies have found the major metabolic way of clivorine in male rat liver microsomes was to form pyrrolic metabolites. The above results suggested that there is sexual difference of metabolism in rats. The major in vitro metabolic way of clivorine in guinea pig was hydrolysis.

FMO的特异性抑制剂methimazo1e可以显著地抑制〓的形成，但对〓的形成无明显的抑制作用，且〓在肝微粒体中的形成为NADPH非依赖性，上述结果提示参与〓和〓代谢的酶分别为肝微粒体中的水解酶和黄素单氧化。

Terminal oxidative inhibitors, such as KCN, Salicylhydroxamic acid , antimycin A and NaN〓, can cut off electron transport chains of cytochrome pathway and alternative pathway, marked decreased BADH protein levels. Oxidative phosphorylation uncoupler 2, 4-dinitrophenol had a similar negative effect on BADH gene expression, and exogenous ATP and ADP increased the BADH levels. These results, in conjunction with the terminal oxidation results, indicated that ATP could be a critical component in BADH gene expression.

2.2 末端氧化抑制剂KCN、SHAM、抗霉素A和NaN〓能打断呼吸链，阻断电子传递，使BADH蛋白的表达量明显降低，说明末端氧化同样参与BADH基因表达的调节：末端氧化影响基因表达的实质就是ATP的影响，线粒体磷酸化解偶联剂DNP明显降低BADH蛋白的含量，而外源的ATP和ADP对BADH基因表达的正效应作用最大。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！