抑素的

Objective: The purpose of our studies are gaining a better insight into the effect of genistein on mesangial cell"s phenotype change and ECM expression plus degradation under DN circumstances.We add genistein with different concentrations in high glucose cultured rat mesangial cells;feed DM rats with the single concentration genistein for different days;to explore the expression of SMemb,the mark of mesangial cell"s hypertrophy phenotype change;to examine the synthesis of Fn as well as the change of the balance between MMP-2 and TIMP-l;to comprehend its effect on renal pathological changes of DM rats and some related factors;trying toexplore the possible mechanisms.Through these studies,we will have some information about the experimental value of genistein in DN field.

目的：为了研究金雀异黄素对DN环境下大鼠MC肥大表型转换标志物SMemb和ECM分泌及降解的影响，我们拟在细胞水平以不同浓度的金雀异黄素作用于高糖培养下大鼠MC，在动物水平以同一浓度的金雀异黄素作用于DM大鼠不同时间，观察细胞表型转换标志物SMemb、ECM主要成分Fn、ECM降解酶系统基质金属蛋白酶—2(MMP-2)／基质金属蛋白酶抑制剂—1(TIMP-1)表达的变化，同时观察金雀异黄素对DM大鼠肾脏病理及影响肾脏病进展的糖脂代谢紊乱的影响，并进一步探讨金雀异黄素发挥作用的可能机制；为明确金雀异黄素在DN治疗中的地位及意义提供实验依据。

Soy protein isolate films were prepared with vanillin and their antimicrobial activity was studied.

对香兰素和香兰素添加到大豆分离蛋白膜后的抑菌效果进行研究。

The detection method comprises the steps that: conditions used for an Oxford cup method to determine polyxin B are established, standard solution of the polyxin B is used to draw a linear regression curve under the condition of biological detection, and then an Oxford cup is utilized to identify the size of a bacteriostatic ring produced by zymotic fluid at each stage, thereby measuring the content of the polyxin B in the zymotic fluid.

本发明公开了一种多氧霉素B快速定量分析的检测方法，该检测方法包括：建立牛津杯法测定多氧霉素B的条件，并用多氧霉素B的标准溶液在生物检测条件下绘制一条线性回归曲线，然后利用牛津杯鉴定各阶段的发酵液所产生的抑菌圈大小，从而测得多氧霉素B在发酵液中的含量。

This study aims at the expression of Wnt signaling pathway inhibitor FrpHE and DKK-1 induced by ectogenous factor.

本课题的目的在于研究外源性因素对Wnt通路抑制剂FrpHE和DKK-1的诱导表达，我们选择抗癌药物p53及化疗药阿霉素、羟喜树碱、顺铂作为外源性诱导剂，以期弄清Wnt通路及其抑制剂FrpHE和DKK-1是否能够被p53和化疗药这两类药物所调节。

Conclusion; Myricetin and quercetin are inhibitors of PI3K, The inhibitory effects against p110β PI3K of myricetin and quercetin are dose-dependent.

杨梅素和槲皮素是PI3K的抑制剂，杨梅素和槲皮素对PI3K p110β催化亚基的抑制作用与浓度成正比关系。

Andres, and its mechanism against rot-causing fungi, the antifungal activity and period of pyrolin against Physalospora piricola and Alternaria alternate was evaluated by using agar dilution method, and ultrastructure changes was observed used by scanning electron microscope.

采用中药抑菌实验方法测定了鹿蹄草素对苹果轮纹病菌和梨链格孢菌的抑菌活性和时期，并在扫描电镜下观察菌体超微结构的变化。

The inhibitory tests in vitro were performed against Escherichia coli, Salmonella pullorum with filter paper method and tube dilution method. And the diameters of inhibition zone, minimal inhibitory concentrations of levofloxacin combined with polymyxin E were measured.

方法］应用纸片法和试管稀释法进行体外抑菌试验，测定左氧氟沙星与多黏菌素联合对鸡大肠杆菌、鸡白痢沙门氏菌的抑菌圈大小和最小抑菌浓度。

Motilin and gastrin induced contraction of isolated gastric smooth muscle cells in a dose-dependent manner.(2) Motilin- and gastrin-induced antral smooth muscle cell contraction was inhibited by antibodies against Gαi-3. Motilin and gastrin caused an increase of Guanosine-5′-(3-［35S］thio)triphosphate(［35S］GTPγS) binding of Gαi-3.(3) Contraction of antral muscle cells induced by motilin and gastrin was inhibited by the phospholipase C inhibitor U-73122 and the IP3 receptor antagonist heparin.

结果显示：(1)胃动素和胃泌素对胃窦平滑肌细胞均有收缩作用；(2) Gαi-3抗体可抑制胃动素和胃泌素加强胃窦平滑肌细胞的收缩，胃动素、胃泌素明显增加Gαi-3抗体与［35S］GTPγS的结合；(3)磷脂酶C抑制剂U-73122、三磷酸肌醇受体拮抗剂肝素可抑制胃动素和胃泌素引起的胃窦平滑肌细胞的收缩。

Methods After treated with a specific demethylating agent,Aza and acetylating agent, TSA, the status of 5'CpC island methylation of ING1b gene in HT29 human colon cancer cell line was analyzed using methylation specific polymerase chain reaction,and the level of histone acetylation was analyzed by chromatin immunoprecipitation,and reverse transcription polymerase chain reactionwas used to examine ING1b mRNA expression.

应用特异性DNA甲基转移酶抑制剂5-氮-2'-脱氧胞苷（5-Aza-2'-dc，以下简称Aza）及组蛋白去乙酰化酶抑制剂曲古抑菌素A作用人结肠癌细胞株HT29后，用甲基化特异性PCR检测该ING1b基因核心启动子区域CpG岛甲基化情况，用染色质免疫沉淀检测其乙酰化组蛋白绑定的DNA情况，并用逆转录聚合酶链反应检测ING1bmRNA表达。

Results ① The inhibitory rate was 68.32% in the curcumine group,70.43% in the positive controlled group .

结果①姜黄素组的抑瘤率为68.32%，阳性对照组的抑瘤率为70.43%，与空白对照组比较，两组的抑瘤率均明显升高(P.01)。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！