抑素

Result Based on directly regenerated plant of in vitro plant of Xinjiang cotton, the cotton epicotyl treated by pre-culture for 3-5 d was immersed in bacteria for 15 min and co-cultured for 2 d. Cephalothin at 100 mg/L was used to inhibit bacteria after 50 mg/L kan selection. And the combination of cytokinin with relatively high content and auxin with low content was used in its directly budding culture to obtain resistant seedlings.

结果］在新疆棉花离体培养植株直接再生植株基础上，经3~5d预培养处理过的棉花上胚轴，在浸菌15 min共培养2 d后，经50 mg/L kan筛选，用1000 mg/L头孢霉素抑菌，并用相对高含量细胞分裂素和低含量生长素的组合对其进行直接出芽培养，从而获得抗性小苗。

ResultBased on directly regenerated plant of in vitro plant of Xinjiang cotton,the cotton epicotyl treated by pre-culture for 3-5d was immersed in bacteria for 15min and co-cultured for 2d.Cephalothin at 1000mg/L was used to inhibit bacteria after 50mg/L kanselection.And the combination of cytokinin with relatively high content and auxin with lowcontent was used in its directly budding culture to obtain resistant seedlings.

结果在新疆棉花离体培养植株直接再生植株基础上，经3～5d预培养处理过的棉花上胚轴，在浸菌15min共培养2d后，经50mg/Lkan筛选，用1000mg/L头孢霉素抑菌，并用相对高含量细胞分裂素和低含量生长素的组合对其进行直接出芽培养，从而获得抗性小苗。

It's has been reported that lactoquinomycin and frenolicin B, analogues of kalamycin, can inhibit AKT for the pyran naphthoquinone skeleton they have. We found that although kalamycin has pyran naphthoquinone skeleton, but did not suppress AKT and its downstream signal system, then we make a sytematic analysis on the in vitro anti-tumor effect and mechanism of kalamycin.

有报道其类似物lactoquinomycin和frenolicin B是肿瘤靶点AKT抑制剂，并由此推断吡喃萘醌骨架在AKT抑制过程中发挥主要作用，我们发现虽然卡拉霉素含有吡喃萘醌骨架，但是并不抑制AKT及其下游信号系统；继而对卡拉霉素的体外抗肿瘤作用及其机理进行了系统的分析。

Transferred the NP-1 gene into C. ellipsoidea by electroporation with high efficiency expression vector pBinUΩNP1. PCR, Southern blot and Northern dot blot analysis of the G418resistant alga strains indicated the stable integration and correct transcription of NP-1 in C. ellipsoidea genome. In vitro antagonistic assays showed the proper expression of defense gene in C. ellipsoidea. Transgenic C. ellipsoidea is antagonistic to gram negative bacteria—Bacillus subtonic, gram positive bacteria—Escherichiacoli and fungi—Fusarium oxysporum to some extent.

以椭圆小球藻完整细胞为受体，采用电激转化法将连接在高效表达载体pBinUΩNP1上的兔防御素NP-1基因导入小球藻，通过PCR、Southern杂交、Northern点杂交对转基因小球藻进行了分子检测，同时通过体外离体抑菌实验检测了兔防御素NP-1基因表达产物的活性，结果表明NP-1基因已稳定整合到小球藻基因组中，并进行了正确转录和表达。

Soy protein isolate films were prepared with vanillin and their antimicrobial activity was studied.

对香兰素和香兰素添加到大豆分离蛋白膜后的抑菌效果进行研究。

Expression of mutant tumor suppressor gene p53 in egg-type chicken with avian leukosis subgroup J was detected to study cancericidally molecular mechanism of ALV-J. Using immunohistochemistry method of strep avidin-biotin complex, many organs of illed egg-type chickens, involved liver, kidney, ventriculus glandularis, tumor, heart, pancreas, bone marrow, spleen, oviduct, lung, duodenum, thymus and bursa of fabricius were studied.

为了探讨J亚群禽白血病病毒的致癌分子机理，观察蛋用型鸡发生J亚群禽白血病时p53抑癌基因在各脏器中的表达，应用链霉亲和素-生物素-过氧化物酶复合物免疫组化法，研究了蛋用型鸡J亚群禽白血病自然病例的肝脏、肾脏、腺胃、肿瘤、输卵管、心脏、胰脏、骨髓、脾脏、肺脏、十二指肠、胸腺和法氏囊。

Alpha-1 antitrypsin is the most abundant circulating protease inhibitor in the body, Dr. Lomas said. Its most common deficiency mutation is the Z allele, where the amino acid lysine is exchanged for glutamic acid at position 342 in the polypeptide chain (Glu342Lys). About 1 in 1700 whites of northern European descent are homozygotes for this mutation and thus have plasma antitrypsin levels that are 10% to 15% below normal, he said.

Lomas博士表示，甲一型抗胰蛋白酵素是身体内最充分的循环蛋白酶抑制剂，最常见的缺损突变在Z对偶基因，该处的胺基酸 lysine与麸氨酸在多胜肽链的第342位置交换(Glu342Lys)；北欧血统白人每1，700人有1人是此一突变的同合子，因此血浆抗胰蛋白酵素值比正常低10%到15%。

METHODS:We recorded the cardiogram by perfusing garlicin injection and garlic preparation to the isolated heart of rabbits by Langendorff method,examined the minimum dose of asystole. RESULTS:The minimum fungistatic concentration of garlicin injection was 3～6.2 times lower than that of garlic preparation.

采用Langendorf法对兔离体心脏分别灌流大蒜素注射液、大蒜注射液、大蒜实验液，分别记录心搏图，求出致心脏停搏的最小剂量，结果：大蒜素注射液的抑菌浓度为大蒜实验液的1／6.2～1／3，而致心脏停搏的最小浓度分别为0.1736u／ml和3.919u／ml。

METHODS:We recorded the cardiogram by perfusing garlicin injection and garlic preparation to the isolated heart of rabbits by Langendorff method,examined the minimum dose of asystole. RESULTS:The minimum fungistatic concentration of garlicin injection was 3～6.2 times lower than that of garlic preparation. The minimum dose of garlic preparation is 0.1736 u/ml, and that of garlicin injection is 3.919 u/ml.

采用Langendorff法对兔离体心脏分别灌流大蒜素注射液、大蒜注射液、大蒜实验液，分别记录心搏图，求出致心脏停搏的最小剂量，结果：大蒜素注射液的抑菌浓度为大蒜实验液的1/6.2～1/3，而致心脏停搏的最小浓度分别为0.1736 u/ml和3.919 u/ml。

Objective: To study the anti-tumor effect and the influence on the level of IL-2 and IL-4 by the decoction of branch of Juglans Maxim in mice bearing S180 tumor cells.

目的：探讨山核桃树枝水煎剂的抑瘤作用及其对S180荷瘤小鼠外周血白细胞介素-2(IL-2)和白细胞介素-4(IL-4)水平的影响。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！