抑制神经

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

The experiment on the effects of 〓 and 〓 on the cerebral artery vasculature revealed that 〓 selectively dilated the pial artery.

离体神经轴突实验探讨了人参皂甙〓对脑缺血保护作用机制中抑制多胺代谢和钙积累之间的主次关系。

Succinylcholine-inducedneuromuscular block had the following characteristics: 1 twitchaugmentation before twitch depression, which was seen more frequentlyin patients given smaller doses (0.1 and 0.3 mg/kg) than inthose given larger doses (0.5–1.0 mg/kg); 2 TOF fadeduring onset and recovery of the block; 3 tetanic fade; and4 and posttetanic potentiation.

琥珀胆碱诱导的神经肌肉阻滞具有以下特点：1）抽搐增强先于抽搐抑制，小剂量（0.1~0.3mg/kg ）比大剂量（0.5~1.0mg/kg ）更明显；2） TOF 衰减贯穿于阻滞的开始和恢复；3）强直衰减；4）强直后易化。

After ICH, PAR-1 is continuously activated because of the stimulation of thrombin. Action of thrombin after ICH may be mediated by PAR-1; NXKT may inhibit the activation of PAR-1, so the praxiology is improved. This may be one of the main mechanisms that NXKT could facilitate the recovery of nervous function.

脑出血后PAR-1受到凝血酶的持续活化，脑出血后凝血酶的作用可能是通过PAR-1介导的； NXKT可抑制PAR-1活化，从而使行为学得到改善，这可能是NXKT促进神经功能修复的主要机制之一。

The computational model that defined the weight of self/other belief by the signal intensity in precuneus was able to predict subject's behavioral delay in the mental inference task.

以上实验结果表明，大脑存在一种独特的与理解他人信念相关的认知神经机制，其主要作用是，通过视角转换抑制基于自我视角获得的信念，这种机制在自我信念和他人信念冲突的条件下，对于正确理解他人信念起决定性作用。

Cells in a part of the brain known as the ventrolateral preoptic nucleus inhibit the activity of all these neurotransmitters.

细胞中的一部分大脑称之为腹外侧视前核抑制活性所有这些神经递质。

It inhibits the stimulation of prothoracicotropic hormone on ecdysteroidogenesis by prothoracic glands in vivo and in vitro.

作为一种重要的昆虫神经肽，能在体内和体外条件下抑制促前胸腺激素对前胸腺的刺激作用。

The present study investigated the effects of pubertal and adult social isolation on latent inhibition of rats and explored its dopamine substrate.

观察青春期和成年早期社会隔离对不同发育阶段大鼠的潜伏抑制的影响，并进一步探讨其神经机制。

to use spinal lateral approach under the guidance of the c-arm x-ray machine, the needle position will be part of the disc, after successful percutaneous insert the plasma sword to melt herniated nucleus pulpous with the power, and then enter the moderate ozone to further expand the scope of ablation, and strengthen the inter-disk degradation and inhibition of the release of inflammatory mediators to eliminate protrusion of nerve roots caused by the oppression and inflammatory stimulation.

采用脊柱后外侧入路，在c型臂x线机引导下，将穿刺针定位于椎间盘部位，经皮穿刺成功后先插入等离子刀，通电后消融髓核使髓核皱缩，再打入适量臭氧，进一步扩大消融范围，加强降解间盘和抑制炎性介质的释放，来消除因间盘突出引起的对神经根的压迫和炎性刺激。

Objective To investigate the change of SSeCKS in sciatic nerve after the transection of rat sciatic nerve and its significance.

目的 探讨大鼠坐骨神经切断后，Src抑制的蛋白激酶C的底物在神经中的表达变化及其意义。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。