抑制真菌的

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全新工艺萃取的茶皂苷、茶皂素、薄荷醇、薄荷异黄酮苷能深层清洁头皮、改善头部角质层、抑制真菌，可祛风散热、止痒、去头屑，其丰富的氨基酸及营养成分对因缺乏营养及烫染受损的干燥、枯黄、开叉发质有良好的修复作用，其天然抗氧化能力可持续强韧。

There was no significant effect of U0126 on the filamentous growth and condial production of the 10 tested fungal plant pathogens: S. turcica, Curvularia lunata, Cochliobolus sativus, Fusarium oxysporum, Alternaria solani, Coniothyrium diplodiella, A. alternata, A. porri, Botrytis cinerea and A. brassicae, but the inhibitor can strongly inhibit the spore germination of S. turcica, A.

在PDA培养基上，U0126对玉米大斑病菌、玉米黄斑病菌、小麦根腐病菌等10种植物病原真菌的菌落形态和生长速度没有显著影响，可以形成正常的菌丝、分生孢子，但U0126处理的玉米大斑病菌分生孢子萌发时间晚，芽管短，分生孢子的萌发百分率下降。U0126对分生孢子萌发的抑制程度随着浓度增加而上升，但随着处理时间的延长而下降。

By comparing with the curves of classical antibiotics in Doskochilova system, the antibiotic type of fermentation broths was identified. The antimicrobial tests showed, the inhibition rate of alt the twenty strains were considerably and ten of them reached 80% when the fermentation broths was diluted by 10 times. It showed strongest inhibition effect against Erwinia carotorora (17.75 mm). Organize method tests showed that inhibition rate of fermentation broths of the strain HJ1-2 to Sclerotinia sclerotiorum and Botrytis cinerea was 100% and 71.39%, respectively.

发酵液的抑菌活性测定结果表明，放线菌HJ1-2菌株发酵液对20种供试病原真菌均具有不同程度抑制作用，其10倍稀释液对其中10种病原真菌菌丝生长的抑制率达到80%以上；供试7种细菌中放线菌HJ1-2菌株发酵液对白菜软腐病菌的抑制作用最强，抑菌圈直径为17.75mn；放线菌HJ1-2菌株发酵液对油菜菌核病和番茄灰霉病的抑制率分别为100和71.39%。

Result3 strains of endophytic fungi had growth peak when the pH value was 6.5-7.5,and could grow within pH of 4.0-8.5. Their acid-alkali adaptability was strong,in which the P-3 adaptability was the strongest. P-1and P-2 had the stronger inhibition to the Bacillus subtilis,Escherichia coli and Staphylococcus aureus,and P-3 only had the little inhibition to Escherichia coli. The 3 endophytic fungi all had inhibitory effect on Podosphaera leucotrlcha,Alternaria mali,and Gymnosporangium yamadae Miyabe,in which,P-1 had the better inhibitory ability.

结果3株内生真菌在pH值6.5～7.5出现生长最高峰，在pH值4.0～8.5都可以生长，酸碱适应性很强，其中P-3的适应性最强。P-1和P-2对枯草芽孢杆菌、大肠杆菌、金黄色葡萄球菌都有较强的抑制能力，而P-3只对大肠杆菌有很微弱的抑制能力。3株内生真菌对苹果锈病病原菌、苹果白粉病病原菌、苹果落叶病病原菌都有抑制作用，其中P-1的抑制能力较强。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

On the inhibition to mycelial growth, the 95% ethanol extract had significantly inhibition to P. ultimum, F. oxysporum f. sp cucumber, R. solani and B. cinerea, and at the top treatment concentration of 0.002 g/mL, the inhibition rate reached to 60.32% 58.50%, 55.89% and 55.17%, respectively; But the 95% ethanol extract almost had no inhibition to S. rolfsii, its rate was just 17.19% at 0.002 g/mL.③On the inhibition to sprouting, the 95% ethanol extract had significantly inhibition to B. cinerea, R. solani, F. oxysporum f. sp cucumber and P. ultimum, and at the top treatment concentration of 0.002 g/mL, the inhibition rate reached to 95.24%, 90.48%, 89.47% and 85.71%, respectively; and the rate would exceed 40% to four fungi mentioned above when their concentration was not lower to 0.005g/mL. The 95% ethanol extracthad infirm inhibition to S. rolfsii, its rate was just 43.48% at 0.002 g/mL.

在抑制菌丝生长方面，鱼腥草95%醇提物对豌豆根腐病菌、黄瓜枯萎病菌、马铃薯立枯丝核病菌和大白菜灰霉病菌均有良好的抑制作用，在最高处理浓度为0.002 g/mL时抑制率分别达到60.32%、58.50%、55.89%和55.17%；对辣椒白绢病菌几乎没有抑制作用，在最高处理浓度为0.002 g/mL时抑制率仅为17.19%；③在抑制孢子萌发方面，鱼腥草95%醇提物对大白菜灰霉病菌、马铃薯立枯丝核病菌、黄瓜枯萎病菌和豌豆根腐病菌抑制效果明显，在最高处理浓度为0.002 g/mL时的抑制率分别达到95.24%、90.48%、89.47%和85.71%，且在处理浓度不低于0.000 5 g/mL时对以上4种真菌孢子的抑制率超过40%；对辣椒白绢病菌抑制作用较弱，在最高处理浓度为0.002 g/mL时仅为43.48%。

The results showed that there were significant differences among fungal species or ecotypes in all above index,which varied with soil types.First,six fungi could colonize maize root on three soils,and the fungal colonization rate was higher on soil collected in the region that had been screened for cinnamon soil and brown soil.

结果表明，6种菌株在上述指标上存在显著的种间或生态型差异，土壤与菌株间存在显著的交互作用。6种菌株在3种土壤上都能与玉米形成菌根，在褐土和棕壤上大多数菌株在分离地所在地区土壤类型上的菌根侵染率较高，说明其对该土壤条件的适应性较强；在红壤强酸性土壤条件抑制了菌根真菌的侵染。

The results indicated that some metabolites of mangrove fungal endophytes were potential resources of compounds of new antitumor activities, which are worthy of further research and development.

以上研究结果表明：一些红树林内生真菌的代谢产物对hTopo Ⅰ表现出良好的抑制活性，有望从中发现潜在的抗肿瘤先导化合物。

We show that Bd infection is associated with pathophysiological changes that lead to mortality in green tree frogs.

Jamie Voyles及其同僚报告说，在感染了该真菌的绿色树蛙中，跨皮肤电解质的转运被抑制了50%以上，而蛙血浆中的钠和钾浓度分别减少了20%及50%。

It is the main strain which has been popularized for its high values such as growing fast, high resistance, long fibre etc., which plays a very important role in reclamative afforestation and shelter belt industry.

几丁质酶可以将几丁质水解为N-乙酰氨基葡萄糖，大量研究表明，几丁质酶在植物体内和体外，对病原真菌的生长均有明显的抑制作用。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失