抑制的人

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗；(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力；(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性；(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡；(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型，应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况，明确其靶向性；(6)将荷瘤裸鼠分3组进行放射免疫治疗，分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

N-terminal amino acid sequence determination indicated that it shares 70%, 64% and 56% identity with those of annexin Ⅱ from the African claw toad, red jungle fowl and human, respectively.

该蛋白具有以钙依赖的方式抑制专一性血小板膜糖蛋白Ⅵ受体激动剂———Stejnulxin诱导洗涤人血小板聚集的生物学功能，最大抑制率达48%。

As a result, there are no specific advantages of processing signals in frequency domain, and we propose to directly process signals in time domain. By this way, it is observed that in time domain the signal signatures can be arranged into groups of orthogonal components, leading to a very natural group-wise symbol recovery scheme. To realize this figure of merit, we propose a GSC-based receiver, which also takes into account the mitigation of channel mismatch effects caused by the channel temporal variation and the imperfect estimation.

因此，吾人提出直接於时域中处理讯号，於时域中，吾人发现讯号特徵矩阵能够被分为数个拥有正交元素的群组，能自然地被用来设计群组式讯号侦测技术，为了实现此特性吾人提出一个GSC接收机，其能同时抑制通道时间变化与通道估计错误所产生之通道偏差效应。

METHODS: A series of N-octyl-N'-succinyl chitosan labeled with fluorescein, were incubated with HepG2 liver cancer cells, K562 leukemia cells, A549 human lung cancer cells, and BGC cancer cells respectively and followed by using flow cytometry and enzyme-linked immunosorbent assay to assess the affinity and inhibition of N-octyl-N'-succinyl chitosan for the four kinds of tumor cells already mentioned.

用异硫氰酸荧光素对N-正辛基－N'-琥珀酰基壳聚糖进行标记，再分别与人肝癌细胞(HepG2)、人白血病细胞(K562)、人非小细胞肺癌细胞(A549)及人胃癌细胞共培养，通过流式细胞仪及酶联免疫检测仪测定N-正辛基－N'-琥珀酰基壳聚糖对肿瘤细胞的亲和性及抑制力。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

This sequence compare to the sequence of PI from ShanXi ShouYang buckwheat once be reported by Zhang Zheng and others,the homology is 64%, From this we can conclude that this gene is a new member of PI from Fagopyrum.

该序列与张政等人报道的山西寿阳甜荞蛋白酶抑制剂基因同源性为64％，由此表明该基因可能为荞麦属蛋白酶抑制剂家族的一个新成员。

The anti-tumor effects of the emulsion to HL-60 leukosis, Bel-7402 hepatic carcinoma, and BGC-823 gastric cancer and KB nasopharyngeal darcinoma were studied in vitro test.

通过体外试管实验考察了鸦胆子油乳样品对HL-60人白血病、Bel-7402人肝癌、BGC-823人胃癌和KB人鼻咽癌肿瘤菌株的抑制效果。

Recombinant human Mullerian inhibiting substance suppresses the growth of MISIIR-positive ovarian cancer cells by inducing apoptosis and cell cycle arrest. We predict that rhMIS might be a new target to treat human ovarian malignancies.

重组人苗勒抑制物可以抑制MISIIR蛋白表达的卵巢癌细胞增殖、诱导其凋亡、阻滞细胞周期，这有望成为治疗卵巢癌的一个新靶点。

LDL of serum in healthy person was separated by improve precipitate method.Results:Pae(300,150 mg.kg-1)could reduce significantly the levels of MDA of serum,aorta and liver;Pae(300 mg.kg-1)decreased obviously the content of OX-LDL of plasma;Pae1 000,200,40 μg.ml-1refrained markadly the course of oxidational decorate of LDL of serum in healthy person.

结果：Pae(300，150 mg.kg-1)可明显抑制高脂大鼠血清、主动脉及肝脏脂质过氧化反应；Pae(300 mg.kg-1)显著减少高脂大鼠血浆LDL的氧化修饰；Pae(1 000，200，40 μg.ml-1)对健康人血清LDL的体外氧化反应有极显著抑制效应。

Objective To investigate inhibitory effect of sorafenib combined with cisplatin against HepG2 hepatocellular carcinoma tumor xenografts.

目的 探讨多靶点酪氨酸激酶抑制剂索拉非尼联合顺铂对人肝癌裸鼠皮下移植瘤生长的抑制作用。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。