抑制的人

Nowadays, daily communication systems in our country is still bonded by patriarchism and consanguinity, which leads to social hierarchy, restrain a person's freedom and individuality; centered by moral ethics, which ignores a person's characters, swells conservatism and parochialism, thus restrains a person's development; featured by human sentiment, which eliminates equity in front of law, strengthens the relationship network brewing corruptions and effects a person's forming of independence, justness and candidness.

我国的日常交往体系仍以宗法血缘为纽带，导致了人的等级性，束缚了人的自由，抑制了人的个性；以伦理道德为本位，忽视了人的个体性，增强了人的保守性和狭隘性，限制了人的发展；以"人情世界"为特征，消解了人在法律面前的平等性，强固了腐败滋生的关系网，影响了人的独立性、公正性和本真性的养成。

OBJECTIVE: Three sesquiterpene lactones 1-O-acetylbritannilactone, isoalantolactone and britannilactone were isolated from the roots of Inula helenium and the flowers of Inula japonica. The antitumor activities of the three sesquiterpenoids in the cell lines of HeLa, HEC-1, SHIN3, HOC-21 and HAC-2 were measured, and the relationship between structure and activity and the possible mechanisms were explored.

目的： 观察菊科植物中分离出的3种倍半萜化合物异土木香内酯、1-氧-乙酰大花旋覆花内酯和大花旋覆花内酯对体外培养的人子宫颈癌HeLa细胞、人子宫内膜癌HEC-1细胞、人卵巢透明细胞癌SHIN3及HOC-21细胞和人卵巢囊腺癌HAC-2细胞增殖的影响，研究这3种倍半萜化合物的体外抑制肿瘤细胞增殖作用及其结构与活性之间的构效关系，进而探讨3种倍半萜化合物的体外抑制肿瘤细胞增殖的可能机制。

Human/rat homology collagen type I siRNA plasmid not only direct repress collagen type I gene expression in rat inesangial cells, but also have the same inhibition action of TGF-β1 induced overexpression of collagen type Ⅰ.

本实验构建的人鼠同源Ⅰ型胶原siRNA质粒，在大鼠系膜细胞内不仅能够直接抑制Ⅰ型胶原的基因表达，而且对TGF-β1诱导的I型胶原表达增加同样具有较好的抑制作用。

Analysis of nuclear extracts of human cells has indicated a similar excision repair mechanism for nick-directed mismatch correction in higher cells, and several reconstituted systems that rely on purified human proteins have been described, such as hMutSα、hMutSβ、hMutLα. Recently, scientists discovered that nickel could be inhibitors of DNA repair. Nickel can greatly enhance the mutagenicity and genotoxicity of compounds in cigarette by inhibiting the nucleotide excision repair pathway in human cells. We have confirmed that nickel could inhibit MMR of E. coli and human and presumed that nickel inhibited repair before MutH incision of the MMR in E. coli. However, there is no evidence of mechanism of nickel inhibiting MMR in human cells.

镍是存在於香菸中的微量金属，近年来有学者发现镍是DNA修复反应的抑制剂，长期抽烟的人会容易因为香菸中化合物攻击DNA而引起突变，进而引起肺癌，而镍会抑制核苷酸移除修复系统（nucleotide excision repair；NER），所以使得体内的DNA损伤无法修复；过去实验室已经证实大肠杆菌的核酸配对错误修复系统会受到镍等重金属的抑制，并且推测出镍的抑制反应是抑制在修复反应的上游nicking的步骤；另外，也证实了人类的核酸配对错误修复系统也会受到镍的抑制，然而还没研究证实会抑制在核酸配对错误修复反应的哪个步骤。

The hydroxy and nitro-substituted coumarins were able to inhibite hemolysis of human red blood cells and damage of DNA induced by AAPH. It reveals that these compounds are effective antioxidants. Some coumarins have cytostatic and cytotoxic activity for HL-60 cell lines. In general, their effects were weakened when coumarins own nitro group.

含有羟基和硝基的香豆素衍生物对自由基诱导的人血红细胞溶血有一定的抑制作用，且对AAPH诱导的DNA损伤有不同程度的保护作用，部分香豆素衍生物对人早幼粒白血病细胞（HL-60）的增殖表现出一定的抑制作用。

In vitro,Mifepristone significantly inhibited the growth and the proliferation of human cervical carcinoma cell lines that are progestin receptor positive and reduced the expression of Ki-67 antigen of the cells.

米非司酮体外对PR阳性的人宫颈癌细胞有明显的抑制作用，通过降低细胞Ki-67抗原的表达而抑制其增殖。

The Interference of cell cycle and proliferation of BGC-803 and Hela cell by water extraction of Agrimonia is studied by electronic microscopy and flowcytometry respectively, and the IL-12 and TGF-β are also detected by ELISA in order to explore the pharmacological mechanism of Agrimonia.

仙鹤草抗肿瘤研究应用流式细胞技术和电镜技术，在体外分别研究了仙鹤草水提取液对胃癌BGC-803和宫痉癌Hela细胞的生长抑制作用和细胞周期的影响，同时检测对免疫活性因子TGF-β和IL-12表达的影响，阐明其对人癌细胞生长抑制的药理机制。

Results:Three different concentrations of Mifepriston dose dependently inhibited the proliferation of He...

米非司酮体外对PR阳性的人宫颈癌细胞有明显的抑制作用，通过降低细胞Ki 6 7抗原的表达而抑制其增殖。

Nowadays, daily communication systems in our country is still bonded by patriarchism and consanguinity, which leads to social hierarchy, restrain a person's freedom and individuality; centered by moral ethics, which ignores a person's characters, swells conservatism and parochialism, thus restrains a person's development; featured by human sentiment, which eliminates equity in front of law, strengthens the relationship network brewing corruptions and effects a person's forming of independence, justness and candidness.

我国的日常交往体系仍以宗法血缘为纽带，导致了人的等级性，束缚了人的自由，抑制了人的个性；以伦理道德为本位，忽视了人的个体性，增强了人的保守性和狭隘性，限制了人的发展；以&人情世界&为特征，消解了人在法律面前的平等性，强固了腐败滋生的关系网，影响了人的独立性、公正性和本真性的养成。

Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果：经槲皮素处理的人结肠癌SW480细胞数量减少，部分细胞体积缩小，细胞膜完整，胞浆浓缩，核染色质固缩，细胞核碎裂，形成凋亡小体；MTT法检测显示当作用浓度为30μmol/L~90μmol/L时，槲皮素对人结肠癌SW480细胞的生长有抑制作用，其抑制作用随着作用浓度的增加和作用时间的延长而增强；流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期，大部分细胞被阻断于S期，随药物浓度的升高和作用时间的延长，G0/G1期细胞比例逐渐增加，S期细胞比例逐渐减少；酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9，随浓度的升高，MMP-2及MMP-9的分泌量减少；免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后，Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。