抑制的

Two models have been proposed to explain molecular mechanism of Dkk's inhibition role in canonical Wnt pathway: in the first model, Dkk1 competes for binding of Wnt co-receptor LRP6; in the second model, Dkk1 can form a ternary complex with LRP6 and Kremen at the presence of Kremen, and triggers the endocytosis of this ternary complex quickly. The controversies between these two models exist during these years.

现今有两种模型分别解释Dkk抑制经典Wnt信号的分子机制：在第一种模型中，一些研究者认为Dkk1主要通过在细胞外与配体Wnt竞争受体LRP6从而抑制Wnt信号；在第二种模型中，另一些研究者们则认为在Kremen存在的情况下，Dkk1通过诱导形成Dkk1-LRP6-Kremen三元复合物，并迅速诱导这个三元复合物的内吞从而发挥抑制功能。

It indicated that the yield of tetraploidy not more than 25% induced by inhibiting the first mitosis with CB, colchicine and 6-DMAP, respectively, while it can be induced with higher percentage by blocking PB1releasing with CB. The tetraploidy percentage of groups shock began at 15—22min after fertilization are higher than that shock began at 42min, and the yield of tetraploidy also increased with the CB density and time lasting.

结果表明，CB、6-DMAP和秋水仙素抑制扇贝第一次卵裂诱发四倍体的比例低于25%；采用CB抑制PB1可有效地诱导产生四倍体，从授精后42min提前到15—22min开始处理，抑制PB1的放出有助于提高四倍体的比例，在12℃，处理开始和终止时间分别在授精后20—22min和62—67min时(即PB2始出现时)，面盘幼虫四倍体率最高，为56.5%。

Through active immunization of Inhibin antige n from porcine seminal plasma and super ovulation of allochthonous purified FSH, the effect of IB active immunization on cow was studied and its mechanism was al so further ascertained by examination of relative hormone concentrations in seru m at different physiological periold.12 Hostein cows and 12 Xiza young cows of 3 -5 years old (postpartum 50 d and above),were selected and divided into the exp e rimental group and control group randomly according to the estrum order.

张志胜 ，田树军，王志刚，桑润滋用猪精液抑制素抗原主动免疫供体母牛，结合外源纯化FSH超排处理，观察了抑制素主动免疫对牛超排效果的影响，并通过检测不同生理时期血液中相关生殖激素的水平，探讨了抑制素免疫影响牛超排效果的机制。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

It is Concluded that the mode of action of pyrimidine benzylamine differs from the other typically commercial ALS inhibitors, such as sulfonylurea and pyrimidine salicylic acid herbicides. Its herbicidal activity is activated by the metabolic processes in the plants.

离体条件下，化合物104-4 IC_(50)＞100 mg／L，而除草剂双草醚IC_(50)值为10~(-5)～10~(-4)mg／L，说明化合物104-4对离体ALS没有抑制作用；活体条件下，化合物104-4对ALS有一定的抑制作用，且随着处理时间的延长，酶活力降低，对ALS的抑制作用增加。

Our research suggests that the cholic acids depress the growth rate of HUVEC with time-concentration dependence.

结论胆酸对HUVEC的生长率有很强的时间-浓度依赖性抑制作用，这种抑制作用除了直接损伤内皮细胞外，还可能与其抑制内皮细胞本身的分泌VEGF有关。

It indicates that the raw materials can be stored in temperature of 0-4℃ for 60 days, they must be immersed in clean water for more than one hour before processing, and peeling be carried out in flowing water; that color preserving solution should be 1.0% NaCl+0.2% citric acid+0.5% Vitamin C+110ppm dichloro isocyanuric acid sodium; that combined browning inhibiting solution is conspicuously effective, and immersion time better be around 20 minutes; that sterilization solution ought to be 200ppm dichloro isocyanuric acid sodium+0.5% ascorbic acid+0.3 citric acid, and 10 minutes' immersion will achieve the effect of sterilization and inhibiting browning; and that after 20-21 minutes of quick-freezing in temperature of -31℃~-32℃, the products can be preserved under -18℃ for a whole year.

通过对速冻牛蒡加工过程中原料储存、削皮方法、护色、浸泡时间、无热杀菌、包装形式等参数进行研究表明：原料0-4℃可储存60天，加工前用清水浸泡1小时以上，削皮时在流水中进行，护色液筛选的1.0% NaCl＋0.2%柠檬酸＋0.5%维生素C十110ppm二氯异氰脲酸钠；复合褐变抑制溶液在抑制褐变方面有显著的效果，浸泡处理时间为20分钟为宜；杀菌液为200ppm二氯异氰脲酸钠＋0.5%抗坏血酸＋0.3柠檬酸，浸泡10分钟即可达到灭菌和抑制褐变的作用；经-31℃~-32℃温度下速冻20~21分钟，产品在-18℃条件下可储存一年。

With butyl xanthate as a collecter, the flotation behavior of jamesonite, pyrrhotite and marmatite, the depressing effects of small mercapto organic depressant (mercaptoacetic acid, mercaptoethanol ) and the effect of Cu2+ ion on depressing ability of mercapto compound were studied through flotation test and infrared spectrum.

摘 要：通过浮选实验、红外光谱测定，考察了脆硫锑铅矿、磁黄铁矿和铁闪锌矿在丁黄药作用下的浮选行为和巯基类小分子有机抑制剂对三种矿物的抑制效果以及Cu2+离子对巯基化合物抑制效果的影响。

Objective To study the relationship between dose of exposure to 50 Hz magnetic field and its effects of suppression to gap junctional intercellular communication function.

结果工频磁场对GJIC的抑制作用与其强度有关，低强度（005、02和04mT）的磁场辐照24小时不能抑制GJIC，高强度（08和16mT）的磁场辐照24小时能够抑制GJIC。

The cytomorphological changes of K562 cells were observed in the electron microscope.Results RRTJ at the concentration of 1μL/mL expressed obviously inhibitory effect on the growth of K562 cells.The inhibitory effect at the concentration of 2μl/ml went up to its highest at 83.4%.RRTJ showed virulence to K562 cells.The karyon changed because of RRTJ attending.

结果 每mL培养液含1μL刺梨汁即对肿瘤细胞增殖有明显的抑制作用，每mL培养液含2μL刺梨汁抑制作用达到最大，为83.4%，再加大剂量抑制作用无明显升高，各种剂量的刺梨汁对细胞均无毒性，刺梨汁的加入使得细胞体积明显变小，细胞核形态发生改变，异染色质增多。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。