抑制的

He CD4〓 T-cell viability determined by the trypan blue test was over 90%. PBMCs from the recipients, after inactivated, were added in 96-well culture plates with donor CD4〓 T-cell cocultured at 37℃ for 24 hours, TJU103 and CTLA4-Ig were given for additional 48 hours coculture. The CD4〓 T-cell was collected and washed as anergic CD4〓 T-cell.

JU103联合CTLA4-Ig应用对供者T细胞增殖活性的影响根据TJU103有剂量限制性特点，选择TJU103最大抑制效应浓度50μg/ml，与不同浓度CTLA4-Ig（1μg/ml、10μg/ml、50μg/ml、100μg/ml）联合，对供者T细胞的增殖抑制率分别为（59.5％、90.5％、93.4％、95.8％），TJU103（50μg/ml）与低浓度CTLA4-Ig（10μg/ml）联合，增殖抑制率在90％以上，已接近最大增殖抑制率，为二者联合应用的最佳组合，与单独应用组比较，有显著的统计学差异（p＜0.05）。

The in vitro effects of Gln49-PLA_2 were examined using the chick biventer cervicis nerve muscle preparation. Gln49-PLA_2 caused a dosedependent blockade of neuromuscular transmission. The time required to cause 50% blockade was about 150 min at the highest concentration 100μg/ml. This inhibition appeared to be presynaptic in origin as evidenced by the lack of effect of toxin on responses to exogenous acetylcholine in the non-stimulated BCp.

Gln49-PLA_2能够剂量依赖性地抑制颈二腹肌神经信号正常传导并影响肌肉收缩，在100μg/ml剂量下，肌肉收缩幅度在150分钟后达到50%的抑制率；同时，随着Gln49-PLA_2浓度的降低，肌肉收缩幅度的抑制效率也逐渐降低；实验证明Gln49-PLA_2对突触后膜乙酰胆碱受体敏感性无影响，对神经肌肉信号传导收缩的抑制作用位点在突触前膜。

The preliminary bioassary showed that at the concentration of 100μg/mL, the inhibitory rate of compounds Ⅴc to Fusarium graminearum was 81.3%;Ⅳe to Fusarium oxysporium was 83.4%;Ⅴe and Ⅴf to Cercospora sorghi were 79.7% and 72.4% respectively;Ⅳd to Fusarium graminearum and Fusarium oxysporium were 68.9% and 65.7% respectively.

初步生物测试结果表明：在质量浓度为100μgmL下，化合物Ⅴc对小麦赤霉病菌的抑制率达到了81.3%；化合物Ⅳe对马铃薯干腐病菌的抑制率达到了83.4%；化合物Ⅴe，Ⅴf对玉米弯孢病菌的抑制率也分别达到了79.7%和72.4%；化合物Ⅳd对小麦赤霉病菌和马铃薯干腐病菌的抑制率分别达到了68.9%和65.7%。

Lagenarium was higher 60%(for example, Glycyrrhiza yralensis, Lycium barberum and Desmodium styracifolium) as the concentration 0.01g/ml. Especially, the G. yralensis showed the strongest inhibitory effect on it with inhibitory rate of 99.6%. The inhibitory rate of 12 PE against mycelium growth and 15 PE against appressorium formation of C.

供试提取物在质量浓度为0.01 g/ml条件下，甘草、枸杞、金钱草等11种植物的80%乙醇提取物对黄瓜炭疽病菌分生孢子萌发的抑制率大于60%，其中甘草的抑制作用最强，达99.6%；12种提取物对黄瓜炭疽病菌菌丝生长的抑制率大于50%；15种提取物对黄瓜炭疽病菌附着胞形成抑制率达50%。

By comparing with the curves of classical antibiotics in Doskochilova system, the antibiotic type of fermentation broths was identified. The antimicrobial tests showed, the inhibition rate of alt the twenty strains were considerably and ten of them reached 80% when the fermentation broths was diluted by 10 times. It showed strongest inhibition effect against Erwinia carotorora (17.75 mm). Organize method tests showed that inhibition rate of fermentation broths of the strain HJ1-2 to Sclerotinia sclerotiorum and Botrytis cinerea was 100% and 71.39%, respectively.

发酵液的抑菌活性测定结果表明，放线菌HJ1-2菌株发酵液对20种供试病原真菌均具有不同程度抑制作用，其10倍稀释液对其中10种病原真菌菌丝生长的抑制率达到80%以上；供试7种细菌中放线菌HJ1-2菌株发酵液对白菜软腐病菌的抑制作用最强，抑菌圈直径为17.75mn；放线菌HJ1-2菌株发酵液对油菜菌核病和番茄灰霉病的抑制率分别为100和71.39%。

Result3 strains of endophytic fungi had growth peak when the pH value was 6.5-7.5,and could grow within pH of 4.0-8.5. Their acid-alkali adaptability was strong,in which the P-3 adaptability was the strongest. P-1and P-2 had the stronger inhibition to the Bacillus subtilis,Escherichia coli and Staphylococcus aureus,and P-3 only had the little inhibition to Escherichia coli. The 3 endophytic fungi all had inhibitory effect on Podosphaera leucotrlcha,Alternaria mali,and Gymnosporangium yamadae Miyabe,in which,P-1 had the better inhibitory ability.

结果3株内生真菌在pH值6.5～7.5出现生长最高峰，在pH值4.0～8.5都可以生长，酸碱适应性很强，其中P-3的适应性最强。P-1和P-2对枯草芽孢杆菌、大肠杆菌、金黄色葡萄球菌都有较强的抑制能力，而P-3只对大肠杆菌有很微弱的抑制能力。3株内生真菌对苹果锈病病原菌、苹果白粉病病原菌、苹果落叶病病原菌都有抑制作用，其中P-1的抑制能力较强。

Results are as followed:1 Exposure of HELF cells to BP caused c-Jun activation,and increased the activity of MAPK,PI-3K,p53 and cyclin D1 pathway.2 BP-induced c-Jun activation was inhibited by dominant negative mutants of extracellular signal-regulated protein kinase or c-Jun NH_2-terminal kinase,but not by p38,impling that JNK and ERK pathways medicate c-Jun activation induced by BP.3 Overexpression of dominant-negative mutants PI-3K and Akt potently blocked phosphorylations of c-Jun and ERK,but not JNK in response to BP,suggesting that PI-3K/Akt pathway positively regulates BP-induced c-Jun activation through ERK.4 Inhibition of p53 by its chemical or molecular inhibitor markedly increased the phosphorylation levels of c-Jun,Akt and ERK upon BP stimulation,indicating that p53 negatively medicates BP-induced c-Jun activation through PI-3K/Akt/ERK pathway.5 The cell lines expressed TAM67 exhibits no significant affecting normal cell growth properties.6 TAM67 was able to significantly block G_1-S transition and subsequent cell proliferation,suggesting that c-Jun is essential for cell cycle alternations elicited by BP.7 Overexpression of TAM67 impaired BP-induced cyclin D1 activation,decreasing expression of E2F1 and pRb,indicating that c-Jun participates in the modulation of BP-induced activation of cyclin D1/pRb/E2F1 pathway.8 Stably expression of TAM67 led to the increases in the expression levels of p53 and p21,elevating phosphorylation level of p53,clearly indicating that c-Jun regulates p53/p21 pathway activation induced by BRCollectively,PI3K/Akt/ERK pathway mediated BP-induced c-Jun activation through p53-dependent mechanism.

结果显示：1BP刺激细胞可促进c-Jun活化，并伴随着MAPK、PI-3K、p53和cyclinD1通路各组成成分的活性增强。2利用MAPK通路的显性失活突变体分别阻断细胞外信号调节激酶和c-Jun氨基末端激酶活性，均可明显抑制BP诱导的c-Jun活化，但阻断p38活性对BP引起的c-Jun活化无明显影响，提示JNK和ERK通路参与调控BP诱导的c-Jun活化。3过表达PI-3K和Akt的显性失活突变体也可显著抑制BP诱导的c-Jun活化，并降低磷酸化ERK的表达水平，但对磷酸化JNK的表达水平无明显影响，说明PI-3K/Akt通路通过ERK正性调控了BP诱导的c-Jun活化。4p53的化学/分子抑制剂能使BP作用的细胞内c-Jun活性明显增加，并同时诱导Akt和ERK的磷酸化水平的升高，表明p53可通过PI-3K/Akt/ERK通路对BP诱导的c-Jun活化进行负性调控。5随后观察转染细胞的生长情况，发现TAM67对细胞正常生长和形态无明显影响。6稳定表达TAM67可有效抑制BP诱导的S期细胞数的增加，提示c-Jun在BP致细胞周期改变的过程中发挥了重要作用。7TAM67过表达能够抑制BP诱导的cyclin D1活化，降低磷酸化Rb以及E2F1蛋白表达水平，表明c-Jun参与调控BP诱导的cyclin D1/Rb/E2F1通路的活化。8过表达TAM67可使BP刺激的细胞中p53、p21总蛋白以及p53磷酸化的表达水平明显升高，可见c-Jun也参与调控BP诱导的p53/p21通路活化。

The effect of genistein (0.8 mg/kg) was partially inhibited by L-NAME, or by sodium orthovanadate (50 μg/kg), a potent inhibitor of protein tyrosine phosphatase;(2) geni...

GST的这一效应可被L 硝基精氨酸甲酯部分阻断，预先注射蛋白酪氨酸磷酸酶抑制剂正钒酸钠( 5 0 μg/kg)，可部分抑制GST( 0 8mg/kg)引起的效应；( 2 )向肾血管灌注环路中直接注射GST也可剂量依赖性地降低肾动脉的灌流压，预先注射正钒酸钠可完全抑制GST引起的效应，而L NAME对此效应没有影响；( 3 )肠系膜血管灌流环路中注射GST可剂量依赖性地降低其灌流压，这一效应可被正钒酸钠部分抑制，而L NAME对此无影响。

The effect of genistein (0.8 mg/kg) was partially inhibited by L-NAME, or by sodium orthovanadate (50 μg/kg), a potent inhibitor of protein tyrosine phosphatase;(2) genistein also decreased the PP of renal vascular bed in a dose-dependent manner and the effect of genistein was completely inhibited by pretreatment with sodium orthovanadate, but unaffected by L-NAME; and (3) genistein decreased the PP of mesenteric vascular bed in a dose-dependent manner, an effect which was partially inhibited by sodium orthovanadate, but unaffected by L-NAME.

GST的这一效应可被L-硝基精氨酸甲酯部分阻断，预先注射蛋白酪氨酸磷酸酶抑制剂正钒酸钠(50 μg/kg)，可部分抑制GST (0.8 mg/kg)引起的效应；(2)向肾血管灌注环路中直接注射 GST 也可剂量依赖性地降低肾动脉的灌流压，预先注射正钒酸钠可完全抑制GST引起的效应，而L-NAME对此效应没有影响；(3)肠系膜血管灌流环路中注射GST可剂量依赖性地降低其灌流压，这一效应可被正钒酸钠部分抑制，而L-NAME对此无影响。

The effect of genistein (0.8 mg/kg) was partially inhibited by L-NAME, or by sodium orthovanadate (50 μg/kg), a potent inhibitor of protein tyrosine phosphatase;(2) Genistein also decreased the PP of renal vascular bed in a dose-dependent manner. The effect of genistein was completely inhibited by pretreatment with sodium orthovanadate, but unaffected by L-NAME;(3) Genistein decreased the PP of mesenteric vascular bed in a dose-dependent manner, an effect which was partially inhibited by sodium orthovanadate, but unaffected by L-NAME.

GST的这一效应可被L-硝基精氨酸甲酯部分阻断，预先注射蛋白酪氨酸磷酸酶抑制剂正钒酸钠(50 μg/kg)，可部分抑制GST (0.8 mg/kg)引起的效应；(2)向肾血管灌注环路中直接注射 GST 也可剂量依赖性地降低肾动脉的灌流压，预先注射正钒酸钠可完全抑制GST引起的效应，而L-NAME对此效应没有影响；(3)肠系膜血管灌流环路中注射GST可剂量依赖性地降低其灌流压，这一效应可被正钒酸钠部分抑制，而L-NAME对此无影响。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。