抑制的

Genetic analysis showed that the expression of rice coleoptile purple line was influenced by two genes,inhibiting gene I and anti-inhibiting gene A i

遗传分析表明：水稻芽鞘紫线表达受抑制基因I和反抑制基因Ai 的影响，I抑制芽鞘紫线C_A_P_系统中的P基因，Ai 抑制I基因。

Genetic analysis showed that the expression of rice coleoptile purple line was influenced by two genes, inhibiting gene I and anti-inhibiting gene Ai.

遗传分析表明：水稻芽鞘紫线表达受抑制基因I和反抑制基因Ai的影响，I抑制芽鞘紫线C_A_P_系统中的P基因，Ai抑制I基因。

The pretreatment of inhibitor was divided into two groups: discard pretreatment liquid group and reserve pretreatment liquid group. At the same time we also tested the activity of caspase3 when cell was pretreated by VerapamiK YTNF3 or TNF-3 . The results indicated that the blocking effect on Y TNF P or TNF P induced L929 cytolysis was significantly lower in inhibitor pretreatment discarding group than in pretreatment reserved group.

为了探讨细胞因子与受体结合后的信号转导速度及抑制剂发挥作用公的时间效应，我们进行了抑制剂预处理和 Y hF i及 iiiF 6预处理实验，二其中抑制剂预处理又分为抑制剂去除和保留两组。

The results showed that with the normal dosage 60g/hm2, inhibiting rate of the plant height and weight of Taiyu 2, Jinzhenzhu, Taiyu 15, Zinuo and other three breeds are beyond 40% and 30%, and it is obvious that plant growing is inhibited, the leaves etiolate and new leaves twist severely and couldn't grow normally. Inhibiting rate of the plant height of Ziyu 2, Taiyu 2, Liaoyu 18, Ludan 50, Zhengdan 958 and some other varieties are between 30.6% and 38.1%, the sensitivity of these varieties are lower and the plant modalities are not influenced bigger; in addition, Nongda 108, Nongda 2008, Xinyu 958 and Taiyu 12 grow healthy, the resistance to nicosulfuron are higher compared with other varieties.

温室盆栽测定结果表明，在烟嘧磺隆大田常规用量60g ai/hm2下，各品种对药剂的敏感性差异显著，泰玉2号、金珍珠、泰玉15号、紫糯等7个品种对烟嘧磺隆表现较为敏感，株高抑制率均在40.4%以上，鲜重抑制率在31.37%以上，表现为植株生长受到抑制，叶片黄化，新叶牛尾状扭曲不能正常抽出；淄玉2号、泰玉2号、聊玉18、鲁单50、鲁原单14、郑单958等品种的株高抑制率在30.6%～38.1%，敏感性略低；而农大2008、农大108、鑫玉958和泰玉12号生长正常，耐药性相对较高，无明显药害症状。

The mechanism probably is that Pishang can inhibit the growth, movement and adheresion to extracellular matrix and endothelial cell of liver tumor cells, up-regulate the expression of nm23, down-regulate the expression of CD44, MMP-2, and inhibit the tumor neovascular by supressing the vascular endothelial cell, down-regulating the expression of VEGF, damaging the primitive mesenchymal cells and inhibiting neovascular gemmation.

砒霜具有明显的抗肝癌细胞作用，并有一定的抗侵袭与转移作用，其机制可能与其能抑制肝癌细胞增殖、迁移、阻止肝癌细胞与细胞外基质和内皮细胞粘附、上调肿瘤转移抑制基因nm23表达、下调肿瘤侵袭分子CD44、MMP-2表达以及通过抑制内皮细胞增殖、下调VEGF表达、损伤间充质细胞、阻止新生血管芽生等途径从而抑制肿瘤新生血管形成有关。

Analysis of nuclear extracts of human cells has indicated a similar excision repair mechanism for nick-directed mismatch correction in higher cells, and several reconstituted systems that rely on purified human proteins have been described, such as hMutSα、hMutSβ、hMutLα. Recently, scientists discovered that nickel could be inhibitors of DNA repair. Nickel can greatly enhance the mutagenicity and genotoxicity of compounds in cigarette by inhibiting the nucleotide excision repair pathway in human cells. We have confirmed that nickel could inhibit MMR of E. coli and human and presumed that nickel inhibited repair before MutH incision of the MMR in E. coli. However, there is no evidence of mechanism of nickel inhibiting MMR in human cells.

镍是存在於香菸中的微量金属，近年来有学者发现镍是DNA修复反应的抑制剂，长期抽烟的人会容易因为香菸中化合物攻击DNA而引起突变，进而引起肺癌，而镍会抑制核苷酸移除修复系统（nucleotide excision repair；NER），所以使得体内的DNA损伤无法修复；过去实验室已经证实大肠杆菌的核酸配对错误修复系统会受到镍等重金属的抑制，并且推测出镍的抑制反应是抑制在修复反应的上游nicking的步骤；另外，也证实了人类的核酸配对错误修复系统也会受到镍的抑制，然而还没研究证实会抑制在核酸配对错误修复反应的哪个步骤。

The results were as follows: the activity of prawns was highest under the condition of pH 7.0, 35 ℃ using pyrocatechol as substrate; four inhibitors i.e ascorbic acid1 mg·mL~(-1, sodium sulphite2 mg·mL~(-1, phytic acid1 mL·L~(-1 and EDTA0.1 mg·mL~(-1 had some inhibiting effects on the PPO of prawns, in which the effect of phytic acid1 mL·L~(-1 was the best; the PPO activity of prawns increased gradurally during the storage period; the PPO activity of prawns was inhibited in vacuum environment, the lower the storing temperature was, the stronger the PPO activity was.

结果表明：以邻苯二酚为底物，虾PPO活性的最适pH为7。 0，最适温度为35 ℃；4种抑制剂(1 mg·mL-1抗坏血酸、2 mg·mL-1亚硫酸钠、1 mL·L-1植酸及0。 1 mg·mL-1EDTA)对南美白对虾PPO都表现出一定的抑制作用，以1 mL·L-1植酸抑制作用最强；南美白对虾在贮藏过程中PPO活性逐渐上升，真空环境中PPO活性受到抑制，贮藏温度越低，抑制作用越强。

These studies indicate the rationality of our molecular design based on the crystal structure of KARI complex. To obtain more KARI inhibitors with high activities, the molecular docking and 3D-QSAR methods were applied to our further studying of these amidine compounds. Two methods AutoDock3. 0.3 and DOCK4. 0 were used in molecular docking, and the calculate results accord with that of herbicidal tests in general. The CoMFA model from 3D-QSAR analysis provided useful guidance for designing highly active compounds prior to their synthesis.

这些分子作为潜在的KARI抑制剂为基于KARI的农药分子设计提供了有利指导；对部分小分子进行了合成，在除草活性初筛的近三十个化合物中，个别化合物表现出较好的除草活性，其中一个化合物wb1596对油菜和稗草具有较高的生长抑制率，并通过E.coli KARI酶活性抑制试验验证了其KARI酶的作用靶标，此化合物可以作为KARI一个有苗头的抑制剂为后续工作奠定了基础；对发现的有苗头的KARI抑制剂进行了结构修饰和优化，合成了一系列脒类化合物并确证了它们的结构，改造后的化合物与原抑制剂相比大多除草活性相当或更佳，从而表明我们基于靶标结构的分子设计的合理可靠性；对所合成一系列脒类化合物进行了分子对接和三维定量构效关系研究。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

It is showed that the mechanism of XSTM was association with inhibiting expression of cell adhesion molecules of endothelium and inflammatory cell infiltrating into vessel wall, promoting fibrinolytic activity,inhibiting platelet adhesion and aggregation, regulating angiotasis, protecting the normal endothelium structure and function, inhibiting collagen hyperplasia and maintaining elasticity of vascular wall.

实验结果表明：消栓通脉颗粒剂具有显著抑制细胞黏附分子——P-选择素的表达，抑制炎性细胞的浸润，促进纤溶活性，抑制血小板黏附、聚集，调节血管张力，保护血管内皮细胞功能，抑制胶原纤维增生，维持静脉壁弹性等治疗作用。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。