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Earthworms might be a good bioindicator for the contamination of roxarsone to soil. No significant influence of roxarsone contamination upon the biomass and the reproductive ability of earthworms was found, which indicated that earthworms could tolerate heavy contamination of roxarsone. 2. Effects on the important enzymatic system of earthworms The activities of PROD, BROD and MROD in P450 family were inhibited.

对蚯蚓体内重要酶系统的影响洛克沙胂对P450酶系中的PROD(7-戊氧基异吩噁唑酮-O-脱乙基酶)、BROD(7-苄氧基异吩噁唑酮-O-脱乙基酶)和MROD(7-甲氧基异吩噁唑酮-O-脱乙基酶)的活性都有抑制作用,对MROD的抑制作用最明显;在抗氧化酶系统中,对过氧化氢酶在整个试验进程中都有激活作用,而对其他抗氧化酶有时起激活作用,有时起抑制作用,规律性不明显。

In clinical. acetylcholinesterase inhibitors reduce the breakdown of synaptic acetylcholine, have been modestly effective in improving the cognitive deficits of Alzheimer's disease.The goal of this work is to determine enzyme kinetics and mechanisms of acetylcholinesterase and butyrlcholinesterase inhibition by five cardiovascular drugs, lovastatin, simvastatin, amlodipine besylate, nifedipine, and hydralazine hydrochloride, and two benzodiazepines, diazepam and chlordiazepoxide hydrochloride. All drugs in this study are reversible mixed-type inhibitors of acetylcholinesterase and butyrylcholinesterase. The pKi values for acetylcholinesterase and butyrylcholinesterase inhibition by the cardiovascular drugs are linearly correlated with the molecular weights of the drugs with the slopes of 0.005 and 0.0021, respectively. Therefore, van der Waals' interactions between acetylcholinesterase and the cardiovascular drugs are stronger than those between butyrylcholinesterase and the drugs. This is probably due to a smaller active site gorge and a more significant peripheral anionic substrate binding site of acetylcholinesterase than those of butyrylcholinesterase.

本研究之目的系决定乙醯胆碱酯酵素和丁醯胆碱酯酵素被五种心脏血管药物lovastatin, simvastatin, amlodipine besylate, nifedipine、hydralazine hydrochloride和两种benzodiazepines:diazepam和Chlordiazepoxide hydrochloride的抑制作用之动力学及机转,这些药物都是乙醯胆碱酯酵素和丁醯胆碱酯酵素的可逆性、混合型之抑制剂,实验结果显示,五种心血管药物对於乙醯胆碱酯酵素及丁醯胆碱酯酵素抑制之pKi值和药物之分子量呈直线之关系,斜率分别为0.005及0.0021因此丁醯胆碱酯酵素,与心血管药物间之凡德瓦作用(van der Waals' )比乙醯胆碱酯酵素与心血管药物间者弱,这可能原因是丁醯胆碱酯酵素之活性区域比乙醯胆碱酯酵素之活性区域更宽广,但丁醯胆碱酯酵素之周边阴离子区域不及乙醯胆碱酯酵素之周边阴离子区域者明显重要,由於五种心血管药物对於乙醯胆碱酯酵素和丁醯胆碱酯酵素抑制之pKi值存在著线性关系表示此种抑制作用系经过共同之反应机理。

It was known that pyridine and Triton-x100 had a bad effect on the cell growth and lycopene production were studied. The results indicated that pyridine and triton-x100 inhibited cell growth and lycopene synthesis;β-ionone increased cell growth and lycopene synthesis when the concentration was 0.05 ml/L, but when the concentration became higher, there would be a decrease on the lycopene output; span-20 increased cell growth and lycopene synthesis effectively when the concentration reached 2.0ml/L, and the lycopene yield became 2.4×10~(-2) mg/L which was 3-fold than control.

研究了培养基中加入不同浓度的小分子效应物吡啶、Triton-x100、Span-20、β-紫罗酮、异烟肼对番茄红素产量的提高作用,结果显示培养基中加入很低浓度的吡啶(0.1ml/L)就会对菌体产生很强的毒害作用;triton-x100对类胡萝卜素的合成也有很强的抑制作用,试验的浓度为0.2ml/L到5ml/L,结果发现它对菌体的生长和番茄红素的合成都有抑制作用;β-紫罗酮在浓度为0.05ml/L时有很小的促进作用,而浓度升高就有了比较强的抑制效果;span-20在液体培养基中的加量为2.0ml/L时,对番茄红素的合成有很强的促进效果,产量达到了2.4×10~(-2)mg/L,比原始菌株产量提高了2倍,而浓度进一步增大就产生了很强的抑制效果。

In addition, NOS activity decreased with the time prolonged in vitro, when oxyhemoglobin, a scavenger of NO, was applied, the NOS activity kept stable. This result above demonstrated that NO can inhibit NOS activity, and it was the inhibition of NOS activity by NO that may be an important protective mechanism to resist the neurotoxicity by Py.

另外,本实验还发现,在体外实验时,一氧化氮合酶活性可随时间的延长而逐渐下降,当向反应体系中加入氧合血红蛋白去除一氧化氮的作用后,则一氧化氮的抑制作用消失,酶活性保持稳定;以上结果表明一氧化氮对于一氧化氮合酶活性具有抑制作用,而且这种抑制作用,可能是机体对抗拟除虫菊酯毒性作用的一种重要保护机制。

The results were as follows: the activity of prawns was highest under the condition of pH 7.0, 35 ℃ using pyrocatechol as substrate; four inhibitors i.e ascorbic acid1 mg·mL~(-1, sodium sulphite2 mg·mL~(-1, phytic acid1 mL·L~(-1 and EDTA0.1 mg·mL~(-1 had some inhibiting effects on the PPO of prawns, in which the effect of phytic acid1 mL·L~(-1 was the best; the PPO activity of prawns increased gradurally during the storage period; the PPO activity of prawns was inhibited in vacuum environment, the lower the storing temperature was, the stronger the PPO activity was.

结果表明:以邻苯二酚为底物,虾PPO活性的最适pH为7。 0,最适温度为35 ℃;4种抑制剂(1 mg·mL-1抗坏血酸、2 mg·mL-1亚硫酸钠、1 mL·L-1植酸及0。 1 mg·mL-1EDTA)对南美白对虾PPO都表现出一定的抑制作用,以1 mL·L-1植酸抑制作用最强;南美白对虾在贮藏过程中PPO活性逐渐上升,真空环境中PPO活性受到抑制,贮藏温度越低,抑制作用越强。

This subset is hyporesponsive and anergic to antigen stimulation. Recently, it has been demonstrated to participate in the immune deficiency in human immunodeficiency virus infection and has close relationship with the development of acquired immunodeficiency syndrome. Meanwhile, the CD4(superscript +)D25(superscript +)Treg is also infected when the human body is invaded by HIV. The CD4(superscript +)CD25(superscript +)Treg will finally be killed by the mechanism of antibody-dependent cell-mediated cytotoxicity. Because of the decrease of the CD4(superscript +)CD25(superscript +)Treg, it can't function as a immune suppressor as a result of the excess of activation of HIV and gradually exhausting of the T cell, which shows that the CD4(superscript +)CD25(superscript +)Treg plays a inhibitory role in the different stages of the development of the HIV as well as different effects in the pathogenesis of AIDS.

它能够抑制自身免疫病的发生和发展,参与肿瘤免疫的调节,同时在感染和移植免疫中也发挥着极其重要的作用。T细胞的这一亚群具有免疫调节和免疫抑制的特性,新近发现它亦与爱滋病的发生、发展关系密切HIV进入人体后,CD4D25调节性T细胞抑制了机体的免疫效应但它也同时被感染,最终由于细胞毒的作用而死亡由于调节性T细胞数量的减少不能有效的发挥其抑制作用,HIV持续的过度活化使得T细胞逐渐耗竭说明在HIV发生、发展的不同阶段Treg细胞可能都发挥了免疫抑制作用,但是却对HIV感染与爱滋病发病的进程产生了不同的效应。

The expression changes of relating-apoptosis gene proteins (bcl-2, bax) were detected by immunohistochemistry before and after treating with the Agaricus Blazei Murill extract to explore the possible mechanisms of inducing apoptosis for MGC-803 cells in vitro. Results: The Agaricus Blazei Murill extract significantly inhibited the proliferation of gastric cancer cells in vitro and the inhibitive effects were depended on the medicine concentration and treating times. After treating 24 hours on the gastric cancer cells with the morphologic changes of apoptosis with chromatin margination, karyopyknosis, karyorrhexis were found by the light.

结果:(1)通过细胞培养和MTT法,表明长白山姬松茸在体外对MGC-803细胞株有明显的抑制作用,加药组与对照组相比其生长抑制率有显著性差异(P<0.01),而且这种抑制作用呈现浓度和时间的依赖性;(2)通过集落形成率的测定结果表明,加药组与对照组相比其集落形成率和集落形成抑制率有明显差异(P<0.01),说明长白山姬松茸对MGC-803胃癌细胞株有明显抗增殖作用;(3)光镜观察结果表明,加药组可见细胞脱水浓缩伊红染色增强,胞体缩小,内含高度浓缩的胞核呈深蓝色等典型细胞凋亡形态;经AO/EB荧光染色观察结果表明,当终浓度为1.0mg/ml的姬松茸提取物作用于MGC-803胃癌细胞24h后,其凋亡率和破膜率与自然凋亡率与破膜率相比,均有显著性差异,其凋亡率86.3%(P<0.001),破膜率为41.6%(P<0.01),说明姬松茸确实有诱导MGC-803胃癌细胞凋亡的作用,同时也有细胞毒作用,但以诱导细胞凋亡为主;(4)免疫组化结果表明,用药前后凋亡相关基因的BCl-2、Bax蛋白均有显著性的改变(P<0.001)。

The result expressed that under the concentration of 0.2mg/mL, the 6 compounds are all active to Sarcina lutea, ME-1, MT-6, MT-land MT-7 are active to Bacillus megaterium, to Escherichia coli, only MT-2 is active. The dimeric natural products are intensively existed in botany, most of them have the obvious biological activities. They have been concentrated on in recent years, and they are one of the compounds to which are enjoyed great popularity in natural product.

结果表明,在浓度为0.2mg/mL的条件下,六种化合物对藤黄八叠球菌均具有明显抑制作用,ME-1、MT-6、MT-1、MT-7对巨大芽孢杆菌有抑制作用,MT-2对大肠杆菌有抑制作用,对绿脓杆菌和枯草芽孢杆菌六种成分均无抑制作用;二聚体化合物在植物界广泛存在,多数二聚体天然产物具有显著的生物活性,引起人们的重视,是天然产物化学界研究的热门化合物之一。

It was found that 0.25%o potassium sorbate produced a positive inhibition against short G+ spore bacillus with a concentration less than 5xl04cfu/ml. A concentration less than %o potassium sorbate hardly exerted a complete control towards short G- plump bacillus having a population density of 5x104cfu/ml. It was proved that use of l% potassium sorbute never controlled the growth of G+ coccus, G~ spirilla and enterobacter with a population density of 104cfu/ml. 1mmol EDTA completely controlled the growth of G+ short spore bacillus and G+ coccus whose cell density was 5xl04cfu/ml. A level of lmmol EDTA showed a limited inhibition against the growth of G- spirilla with a population density of 105 cfu/ml. However, a level of 10mmol EDTA completely controlled the growth of the G- spiral bacteria having a population density of 105cfu/ml. lOmmol EDTA produced a very significant control towards the growth of G"" plump short bacillus with 105cfu/ml. 20mmol EDTA showed a remarkable inhibition against the enterobacter with a population density of 105cfu/ml. Different concentrations of nisin including 25mg/mL, 50mg/mL, 75mg/mL and 100mg/mL were used as bio-preservative to examine its effects against the growth of all strains leading to the spoilage of fresh mutton meat. It was seen that there was a big difference in nisin's concentrations in inhibiting the spoiling bacteria. Generally speaking, as more as 75mg/mL of nisin significantly inhibited the growth of G+ short spore bacillus, G-plump short bacillus, enterobacter, G'spiral bacteria and G+ coccus having a population density of 105cfu/ml.

分别运用山梨酸钾、EDTA和Nisin对7种主要引起羊肉腐败的微生物进行了抑菌实验,结果显示,0.25‰以上山梨酸钾能够有效抑制5×10~4 cfu/mL以下的革兰氏阳性短芽孢杆菌的生长;1‰以下的山梨酸钾不能完全抑制5×10~4 cfu/mL革兰氏阴性粗短杆菌的生长,对10~4 cfu/mL革兰氏阳性球菌菌株、革兰氏阴性螺旋菌菌株和肠杆菌菌株抑制效果不太明显。1mmoL EDTA能完全抑制住小于10~5 cfu/mL革兰氏阳性短芽孢杆菌菌株、革兰氏阳性球菌菌株的生长,能明显的抑制10~5 cfu/mL的革兰氏阴性粗短杆菌菌株生长,对10~5 cfu/mL的革兰氏阴性螺旋菌菌株有一定的抑制作用。10mmoL EDTA能完全抑制住10~5 cfu/mL革兰氏阴性螺旋菌菌株的生长;能明显抑制10~5 cfu/mL的革兰氏阴性粗短杆菌菌株的生长,而20 mmoL EDTA能很明显抑制10~5 cfu/mL肠杆菌菌株的生长。25mg/mL、50 mg/mL、75 mg/mL和100 mg/mL的Nisin几乎对所有引起羊肉的腐败菌有抑制作用,但抑制程度不同,抑菌活性有一定的变化。

The mycelial growth of Rhizoctonia solani was promoted by vanillic acid and syringic acid at levels of 0.1~1 mM/L, and it was inhibited remarkably by p-counaric acid of over 1mM/L and modestly by ferulic acid. The radial extent of mycelia of Fusarium solani was increased significantly by ferulic acid of 0.1~1 mM/L and limitedly by p-coumaric acid. vanillic acid had paradoxcial effects on the growth, positively at low levels and negtively at high levels, on which Syringic acid had a slight effect.

试验结果显示:浓度为0.1~1mM/L的丁香酸对西洋参立枯丝核菌菌丝生长具有促进作用,香草酸也有一定的促进作用,但不呈浓度效应,浓度在1mM/L以上的香豆酸对其有显著的抑制作用,阿魏酸也有一定抑制作用;对于西洋参根腐菌,阿魏酸对其生长有明显促进作用,香豆酸也有一定促进作用,香草酸表现为低浓度促进,高浓度抑制作用,0.1~1mM/L丁香酸对其生长基本无影响。

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I didn't watch TV last night, because it .

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