抑制作用的

On the antagonistical mechanism,it is suggested that the poison of Cd on tobacco could be impaired by Fe supply in Cd pollution area.

结果表明：Cd、Fe、Cd+Fe和Fe+Cd处理均不改变氨基酸的种类组成；Cd对烟草叶片氨基酸含量的影响比Fe大；Fe抑制Cd对氨基酸含量的影响，Cd也抑制Fe的作用，Cd与Fe相互拮抗，并初步探讨了拮抗机制；建议在Cd污染地区通过施加Fe来减轻Cd对烟草的毒害。

Results After 4 weeks medication, the gain weight、LH in serum、growth of uterus and ovary be slower significantly. Kangzao-Ⅱ Decoction can reduce asteroidal colloid cell in hypothalamus and acidophil cell in pituitary which secrete FSH and LH.

结果 抗早2号方对青春期雌性SD大鼠的体重增长有抑制作用，能明显抑制血清LH水平，使大鼠的子宫腺体发育延迟，卵巢内卵泡发育滞后，能使青春期雌性SD大鼠的下丘脑星形胶质细胞数量减少，体积缩小，使脑垂体分泌促卵泡生成素和LH的嗜酸细胞减少。

Carotene and bixin inhibited the of MCF-7 human breast cancer cell significantly. The effects of carotenoids were pleiotropic.β-Carotene can activate PPARγ signal pathway and regulate expression of the downstream and relative genes, and impel cytochrome C liberation from mitochndria and increase the level of reactive oxygen species in cell.

胡萝卜素与胭脂树橙显著抑制乳腺癌MCF-7细胞的生长，β-胡萝卜素的抑制作用可能是多效性的，一是激活PPARγ信号系统，调节PPARγ下游基因及相关基因表达，另一个是促使线粒体中的细胞色素C释放，引起细胞内活性氧生成增加，导致细胞活力下降或坏死。

In order to overcome the problem of multidrug resistance in human epidemic carcinomata anti-adriamycin cells (KB-A-1), the antisense and antigene oligonucleotides were used to investigate their effectiveness on inhibiting the mdr1 gene expression. The effectiveness of antisense or antigene oligonucleotide on inhibiting the multidrug resistance was detected by MTT colometric assay and ELISA.

中文题名反义与反基因寡核苷酸及其萘二酰亚胺偶联物对靶基因表达的抑制作用研究副题名外文题名 The inhibition of the targeted gene expression by the antisense or antigene oligonucleotides and their naphthylimide-conjugated derivatives 论文作者李军生导师张元兴魏东芝教授学科专业生物化工研究领域\研究方向生物化学与分子生物学学位级别博士学位授予单位华东理工大学学位授予日期2001 论文页码总数106页关键词基因表达基因治疗反义寡核苷酸反基因寡核苷酸核酸馆藏号BSLW /2001 /Q78 /264 针对人表皮癌抗阿霉素细胞株（KB-A-1）的多药抗药性问题，本文从反义核酸和反基因核酸角度，通过MTT法检测细胞生长情况，ELISA法检测基因表达产物P-gp表达水平的变化，对寡核苷酸抑制肿瘤细胞MDR1基因表达的机制进行了探讨。

Chrysin can inhibit the infection and replication of HIV1 and activation of CD4+T in vitro.

CR体外对HIV1的感染和复制有一定的抑制作用，且可抑制抗原刺激的CD4+T细胞的活化。

The best relative effect of cross-protection was found when spore concentration of Cladosporium cucumerinum was adjusted to 1×10^2 spores/ml for 48 h interval. And it was much better in resistant cultivars than in susceptible ones. Meanwhile, after challenged by Corynespora cassiicola the cucumber induced by Colletotrichum orbiculare produced great resistance to brown-spot, but that increased the development of anthracnose.

在黑星病菌对霜霉病菌的诱导作用中，以浓度为1×10^2个／ml的黑星病菌孢子悬浮液的诱导效果最好，诱导间隔期为48h黄瓜黑星病菌对霜霉病的交互保护作用最明显，抗性品种的交互保护作用明显好于感病品种；炭疽病菌可诱导黄瓜有效抑制褐斑病的发生，但挑战接种褐斑病菌后，却促进了炭疽病的发生。

The results showed that resveratrol dose dependently inhibited the expression of these two genes in a concentration range from 5μmol/L to 50μmol/L.

结果表明白藜芦醇在5~50μmol/L的浓度范围能显著的抑制TNF-α和iNOS的表达，白藜芦醇的上述抑制作用呈浓度依赖性。

Daphne diterpene esters are principal activitive components of Daphne genkwa, which is an endemic traditional Chinese medicine. Yuanhuacine has potent inhibition against several cancer cell lines. Inhibition against DNA topoisomerase I is probably its anticancer mechanism.

芫花为中国特有的瑞香科植物，主要有效成分为植物中罕见的瑞香烷型二萜原酸酯类化合物，其中芫花酯甲对多种癌细胞系有较强的抑制活性，对DNA拓扑异构酶I的抑制作用是其抗癌的可能机理。

RESULTS:Isoalantolactone exhibited excellent anti-proliferative activities on HeLa, SHIN3 and HOC-21 and HAC-2 cell lines, while 1-O-acetylbritannilactone and britannilactone demonstrated weak anti-proliferative activities on HeLa, SHIN3, HOAC-21 and HAC-2 cell lines even at the concentration of 100 μmol/L. The apoptotic rate in isoalantolactone group with the concentration of 12.5 μmol/L was higher than that in PBS group.

结果： 异土木香内酯对上述5种肿瘤细胞的增殖均有明显的抑制作用，1-氧-乙酰大花旋覆花内酯和大花旋覆花内酯即使在100 μmol/L的高浓度下，对5种妇科肿瘤细胞的增殖不显示抑制活性；流式细胞学检查发现异土木香内脂处理后的HeLa细胞出现凋亡现象。

The results showed that expression plasmid pET22b-lysB was constructed successfully. Highly purified recombination protein was obtained 33.2 mg from 1 L LB culture medium. A screening for His-LysB activity on esterase and lipase substrates confirmed the lipolytic activity. With p-nitrophenyl butyrate as substrate, the thermal stability of the enzyme was poor when the temperature was above 30oC. The enzyme exhibited higher stability at pH 5.0–9.5. The optimum temperature and pH for the lipolytic activity of His-LysB were 23oC and 7.5 respectively. Under the optimum conditions, the specific activity of His-LysB was 1.3 U/mg. Zn2+, Cu2+, Mg2+, Mn2+and phenylmethane sulfonyl fruoride severely inhibited the lipolytic activity of His-LysB.

结果表明：成功构建了pET22b-lysB表达载体，并从1 L的LB培养物中获得了33.2 mg高纯度重组蛋白；His-LysB具有分解脂肪的能力，属于脂肪酶；生物化学特性分析表明：丁酸对硝基苯为水解底物，His-Lys热稳定性不佳，30℃以下比较稳定，随着温度的升高，稳定性逐渐降低；该蛋白具有较高的pH值适应性，pH 5.0~9.5范围内稳定性较高；在23℃和pH 7.5时酶活力最高，其比酶活为1.3 U/mg；金属离子Zn2+、Cu2+、Mg2+、Mn2+和苯甲基磺酰氟抑制剂对酶活具有强烈的抑制作用。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。