抑制作用的

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

The hydroxy and nitro-substituted coumarins were able to inhibite hemolysis of human red blood cells and damage of DNA induced by AAPH. It reveals that these compounds are effective antioxidants. Some coumarins have cytostatic and cytotoxic activity for HL-60 cell lines. In general, their effects were weakened when coumarins own nitro group.

含有羟基和硝基的香豆素衍生物对自由基诱导的人血红细胞溶血有一定的抑制作用，且对AAPH诱导的DNA损伤有不同程度的保护作用，部分香豆素衍生物对人早幼粒白血病细胞（HL-60）的增殖表现出一定的抑制作用。

Analysing ustiloxins inhibiting plant cell mitosis The inhibition experiment of growth of garlic radicle showed that there were more cells in the telophase stage in the garlic radicle dealed with culture filtrate of Ustilaginodea virens than those dealed with H2O. The cell volume of the former was bigger than that of the latter. So it seemed that ustiloxins could inhibit the cell mitosis, but not in the cell elongation.

毒素对植物细胞分裂的抑制作用分析：用大蒜根尖做抑制试验，发现用稻曲病菌培养滤液处理过的大蒜根尖呈分裂末期的细胞数目明显多于相同条件浙江大学硕士学位论文下对照的数目，抑制率为69%，且细胞明显比对照的细胞大，说明毒素抑制根尖细胞的有丝分裂，但不能抑制细胞的伸长。

objective effects of plant tannins,chebulinic acid and tellimagrandin i on chemically induced hemoglobin synthesis in k562 cells were investigated.methods the hemoglobin synthesis situation was assayed with benzidine staining.erythroid antigens glycophorin a expression on the surface of k562 cells was labeled by direct immunofluorescence using fluorescein isothiocyanate-conjugated anti-gpa antibodies.then flow cytometric analysis was performed to detect gpa expression levels on surface of the cells.results both chebulinic acid and tellimagrandin i could inhibit the hemoglobin synthesis of butyric acid and hemin-treated k562 cells in a concentration-dependant manner.however,the ba-induced k562 cells were more sensitive to two tannins than heroin-induced cells.conclusion chebulinic acid and tellimagrandin i have inhibitory effect on the erythroid differentiation.

目的 研究可水解单宁诃子酸和特里马素i对氯化高铁血红素和丁酸钠诱导k562细胞红系分化的影响。方法四甲基偶氮噻唑蓝法分析诃子酸和特里马素i对k562细胞生长的影响，联苯胺染色法检测血红蛋白合成情况，应用免疫荧光和流式细胞术检测血型糖蛋白a在细胞表面的表达。结果诃子酸和特里马素i在0.04～0.09？mmol／l浓度下均可显著抑制k562细胞的生长增殖。2种单宁化合物(0.002～0.01？mmol／l)可显著抑制丁酸钠诱导的k562细胞血红蛋白合成，2种单宁化合物(0.01～0.05？mmol／l)还可显著抑制氯化高铁血红素诱导的血红蛋白合成，特里马素i(0.01？mmol／l)还抑制丁酸钠诱导的gpa在细胞表面表达。结论诃子酸和特里马素i对红系分化有抑制作用。

Seven grapevine cultivars originated from different regions, i. e.'Carbernet Sauvignon' and'Queen of Vineyard'(two members of the Western Europe Cultivars Group),'Rizamat'(a member of the East Cultivars Group),'Red Double Taste'(a hybridized cultivar from Vitis vinifera L. and V. labrusca L.),'1103Paulsen','Red Globe'and 'Autumn Royal'(two new hybridized cultivars between cultivars groups which were warmly planted in recent years) were selected. After different drought stress, a combined measurement of gas exchange and chlorophyll fluorescence was conducted to evaluate the effects of drought stress to photoinhibition, as well as the role of photorespiration and xanthophyll cycle in photoprotective mechanism. After photorespiration and xanthophyll cycle were separately inhibited, the complementary effect of these two photoprotective pathways was discussed. Detached leaves of different grapevine cultivars, i. e.'Cabernet Sauvignon','Cabernet Franc','Merlot', and a rootstock,'1103Paulsen'were selected as materials, and photoinhibition degree, photorespiration activity as well as its photoprotective function under drought stress were studied. The results are as follows: 1. Under the same drought stress treatment, different photoinhibition degrees formed in different grapevine cultivars.

本研究选择不同起源地的葡萄品种－－包括西欧品种群的赤霞珠和葡萄园皇后、东方品种群的里扎马特、欧美杂种红双味、美洲种砧木1103Paulsen，以及近年来发展较快的品种群间杂交新品种红地球和皇家秋天等七个品种盆栽苗，在进行不同程度的干旱胁迫处理后，利用气体交换参数和叶绿素荧光参数同步测定的方法，研究干旱对其光抑制程度的影响，以及光呼吸和叶黄素循环组分在其光保护中的作用；并在分别抑制光呼吸和叶黄素循环的基础上，探讨了赤霞珠葡萄两种光保护机制的互补效应；同时选择不同的酿酒葡萄品种赤霞珠、品丽珠、梅鹿辄和砧木1103Paulsen的叶片，研究其离体干旱条件下光抑制程度、光呼吸活性及其光保护作用，结果发现： 1。

The results of bioassay experiments with 10 commonly used fungi showed that, all the title compounds had satisfactory fungicidal activity against Botrytis cinerea, the growth inhibition rate of compounds 5ae, 5ag, 5bd against Botrytis cinerea were up to 70% and for 5ad, 5cd, 5ce were more than 60%; compound 5bd exhibited fungicidal activity against Pellicularia sasakii with growth inhibition up to 60%, the growth inhibition rate of compound 5cg against Colletotrichum lagenarium was 69%.

抑菌活性生物测定结果表明，合成的大部分化合物对黄瓜灰霉病菌均有较好的生长抑制作用，其中，化合物5ae、5ag和5bd的抑制率达到70%；化合物5ad、5cd、5ce的抑制率大于60%；5bd对水稻纹枯病菌抑制率大于60%，化合物5cg对西瓜炭疽病菌的抑制率为69%。

Its activity was specific and was affected by pH, reaching the peak in weak acidic condition. It obviously inhibits amylases from D. melanogaster, Solenopsis invicta, Odontotermes formosanus, Periplaneta Americana Linnaeus, and Alphitobius sp. In addiction, the study points out Phaseolus vulgaris, Phaseolus coccineus L. and Glycine max, which have significant inhibition toward pancreatic amylase.

经测试发现此抑制剂在85℃时仍具备抑制果蝇淀粉酶之活性，为一热安定性蛋白质，其抑制作用受pH 值影响很大，在偏酸性环境下的效果最好，且其抑制作用具特性，可明显抑制果蝇、入侵红火蚁、白蚁、蟑螂及面包虫等昆虫的淀粉酶活性。

The results showed that 0.01,0.05 and 0.10 g/ml extract of Saxifraga stolonifera Meerb had strong antimicrobial effect on S.aureus,but 0.25 and 0.50 g/ml extract had promotive effect on S.aureus.And the extract of Saxifraga stolonifera Meerb of different concentration had antimicrobial effect on E.coli.

结果表明，0.01、0.05、0.10g/ml 3种较低浓度的提取物对摇床培养的金黄色葡萄球菌生长具有较强的抑制作用，而0.25和0.50g/ml 2种较高浓度的提取物对摇床培养的金黄色葡萄球菌生长有较强的促进作用；这5种浓度的提取物均对摇床培养的大肠杆菌生长表现为抑制作用。

Berbamine could inhibit the growth of leukemia cell line NB4. The induction of cell apoptosis may be one of the mechanisms for suppressing the growth of leukemia cell line NB4. Inhibition of Survivin mRNA and upregulation of Caspase 3 protein might be also involved in cell apoptosis.

小檗胺对NB4细胞有增殖抑制作用，诱导细胞凋亡可能是其抗肿瘤作用的重要机制之一，但不是通过作用于NB4细胞特异性融合基因PML/RARα发挥作用的，其抗凋亡的可能分子机制之一是抑制Survivin基因的表达和增加Caspase3的表达。

Result］High concentration of leachates had significant inhibitory effects,but low concentration of leachates had no obvious effects in the initial stage and later stage after treatment.Low concentration of alcohol leaching liquor might had stimulating effects on SOD active.Water leaching liquor had no obvious effects,but higher concentration of alcohol leaching liquor had significant inhibitory effects on root vigor.

结果］在油松针叶水提液和醇提液处理后的初期（10 d）和后期（30 d）的抑制作用仅在高浓度处理；低浓度醇提液对草坪草'抢手股'SOD活性可能有促进作用；水提液对根系活力没有明显的影响，较高浓度的醇提液对根系活力有明显的抑制作用。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。