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Result: It has been found out that the fat-soluble nonsaponified extract from Temminck et Schlegel and the alcoholic extract from Syngnathus acus have cytotoxic activities.The nonsaponified extract from Temminck et Schlegel can inhibit the grouth of cancer cell strains KB,Hela,PAA,K562,and Bcap37,and the alcoholic extract from Syngnathus acus can inhibit the growth of cancer cell strin KB,But Bloch shows no apparent anticancer activity.

结果:尖海龙的95%乙醇提取物对KB细胞的生长具有抑制作用,并呈量效关系;粗吻海龙的脂溶性非皂化物具有不同程度的抑制人癌细胞株KB,Hela,Bcap37,K562,PAA细胞的作用,并有明显的量效关系;拟海龙的各提取物未见有明显的抑制肿瘤细胞生长的作用。

Results The extract remarkably inhibited the contraction caused by histamine and acetylcholine, as well as the ovalbumin sensitized allergic constriction of isolated ileum smooth muscle of guinea pigs. It could inhibit the increase of capillary permeability induced by acetic acid in mice, inhibit the mice paw edema produced by carrageenin and reduce the production of PGE 2 in inflammatory tissues.

结果:辛夷二氯甲烷提取物对组胺、乙酰胆碱(Ach引起的豚鼠离体回肠收缩具有显著的抑制作用,并能抑制卵白蛋白所致的豚鼠离体回肠过敏性收缩,对HAc引起的小鼠腹腔毛细血管通透性增高具有抑制作用,能显著抑制角叉菜胶引起的小鼠足肿胀,并减少前列腺素E2(PGE2)在炎症组织中产生。

Results showed that L-THP possessed negative inotropic and negative chronotropic action, lowed left ventricular pressure and it's infinitesimal calculus, decreased heart output in per min., shorten 〓 and 〓 of action potential of ventricular papillary muscle of guinea pig and even the plate-phase of action potential disappeared in larger dose group , but prolonged 〓. we studied transmembrane transportation of 〓 after administering L-THP , L-THP not only depressed uptake and increased release of Ca 2+ in normal myocardium, but also it showed stronger inhibition in cell injured by ISO, and fully eliminated the effect of ISO increasing uptake and decreasing release of 〓. By detecting the 〓 content of ventricular single myocyte using fluorescent probe Fluo-3/AM, results correspond to that done above, L-THP decreased 〓 content inside of cell significantly. Using whole-cell mode, we observed the effect of L-THP on Ica in ventricular single cell, it was found that L-THP decreased Ica, the effect increased with increasing it's dose, and had voltage, frequency-dependency. Dose highly corelated to effect, corelative constant is 0.87. When washing cell with normal tyre's solution, Ica could recover, which proved the blocked action of L-THP on calcium channel once again. Meanwhile, L-THP reduced production of MDA in heart injured by ISO in dose-dependent manner, improved content of GSH-Px, in case tissue or cell free from free radical damage.

结果显示,该药物具有负性肌力和负性频率作用,降低左室压及其微分,终使每分心输出量减少;缩短豚鼠心室乳头肌动作电位时程的〓和APD〓,大剂量使动作电位平台消失,〓延长;同位素示踪法研究证实L-THP抑制〓摄取,促进〓释放,降低细胞内的〓含量;单通道水平的研究证实,L-THP减少豚鼠心室肌单细胞内向钙电流,且具有剂量,电压,频率依赖性,其作用易于洗脱,药物剂量与Ica也具有高度相关性(相关系数r为-0.87);L-THP还可抑制ISO损伤心肌组织所致的丙二醛含量增加,增加谷胱甘肽过氧化物酶的量,使组织细胞免受损伤。L-THP阻断L-型〓通道,抑制〓的摄取,促进〓的释放,降低细胞内〓含量L-THP作用的离子基础。

Intravoneous injection of 0.5mg/kgBW jingsongling cause the 60 minutesrelaxation of goat gastric motor.The effects of jingsongling on the gastric motor ofgoat is major of effects of α2 -adrenoceptor agonist,as well as that of α1 -adrenoceptor agonist,the effects of α1 -adrenoceptor agonist concentrate at thecardic and pylorus;the function of α2 -adrenoceptor exit widely at cardic,pylorus,rumen,reticulum,omasum and abomasum,the function at different part is not the same.The lowestdose of jingsonglinginhibiting the amplitude and frequency of the gastricmyoelectric is 0.01mg/kgBW;the influence of jingsonglingto the gastricmotility of goat is dose-dependent,and the amplitude regain after the frequency ofgastric myoelectriv wave.When the goat revived,the gastric motor function is notreach the level of the normal;the major reason ofjingsongling cause the goat gastricrelaxation is the amplitude renew slowly.The intravoneous injection ofjingsonglingfirst,after 5 minutes,intravoneous injection the antagonist,the effect of prazosin and idazoxan mixed is best,which can fast reversal of theinhibition of jingsongling on the gastric motor of goat,the effects of idazoxan isgood,and the prazosin block the effection of jingsongling is better than that ofxylazine,especially at cardia and pylorus.When intravoneous injection of0.5mg/kgBW idazoxan and/or prazosin only,the amplitude of gastric myoelectric ingoat increases at a certain degree,but the frequency is not changed.

静脉注射0.5mk/kgBW的静松灵引起山羊胃蠕动弛缓长达60分钟;静脉注射0.5mg/kgBW的静松灵对山羊胃蠕动机能的影响是以α2肾上腺素受体作用为主的,兼有α1肾上腺素受体的作用,而且静松灵的α1肾上腺素受体的作用主要集中在幽门和贲门;α2肾上腺素受体的作用广泛存在于贲门、瘤胃、网胃、瓣胃、皱胃和幽门中,不同部位间有功能上的差异;静注0.01mg/kgBW的静松灵是对山羊的贲门、瘤胃、网胃、瓣胃、皱胃和幽门的肌电波频率和振幅均产生影响的最低剂量;静注一定剂量的静松灵对山羊胃蠕动的抑制作用与剂量呈相关性;而且山羊胃肌电波振幅的恢复比肌电波频率的恢复慢;苏醒时山羊的胃蠕动机能尚未恢复到正常状态;静松灵引起山羊胃弛缓的主要原因是由于胃肌电波的振幅恢复慢;静注0.5mg/kgBW的静松灵,5分钟后静注0.5mg/kgBW的拮抗剂时,咪唑克生+哌唑嗪的作用最好,能迅速地逆转静松灵对山羊胃蠕动的抑制作用,咪唑克生也有很强的拮抗作用,而哌唑嗪对静松灵的拮抗效果好于对隆朋的拮抗;单独静注0.5mg/kgBW的咪唑克生和/或哌唑嗪时,山羊的瘤胃、网胃、瓣胃、皱胃、贲门和幽门的肌电波振幅有一定程度的增强,而肌电波频率未见变化。

The results indicated that the actual inhibition ratio and the theoretical inhibition ratio of net photosynthate and stomatal conductance of glyphosate-ammonium and fluroxypr-mepthyl (4:1) mixture against Altemanthera philoxeroider were from 80% to 100%, and those of intercellular CO2 concentration, content of chlorophyll and malondialdehyde were above more than 110%, and strong synergism.

结果表明,该配比混剂处理对空心莲子草净光合速率和气孔导度的实际抑制率与理论抑制率的百分比在80%~100%之间,表现出相加作用;而对胞间CO2浓度、叶绿素含量及丙二醛含量的实际抑制率与理论抑制率的百分比均在110%以上,表现出较强的增效作用。

Furthermore, in the CTA rats, compared with water stimulability, FLI neurons of SON and DMV induced by sucrose stimulability had had much more expressions.6 When the same sucrose stimulability was given, FLI neurons expressions of SON, DMV and LH in the CTA rats were much lowwer than that in the control rats.Conclusions:1 CTA had an inhibitory effect on small intestine peristalsis, indicate taste preference has an effect on small intestine peristalsis, and inhibitory effect induced by CTA is corresponded with US, this effect may have relations with vagus efferent never and humoral regulation.2 In the electrophysiology experiment, discharges of vagus efferent never in the CTA rats are much more than that in the control rats, indicates taste and taste preference may regulate small intestine peristalsis via vagus nerve, and dicharge can continude 1 hour, indicate humoral regulation also exists in the regulation.

结论1建立味觉厌恶对小肠运动有抑制作用,提示味觉嗜好性对小肠运动有影响,而且味觉厌恶引起抑制作用是与条件刺激成对应关系的,味觉刺激引起的小肠蠕动的抑制作用可能与迷走神经传出神经及体液调节有关。2在电生理实验中,CTA大鼠迷走神经传出放电高于对照组的大鼠,提示味觉及味觉嗜好性可能是通过迷走神经对小肠运动进行调节的,放电至能持续60min,表明味觉及味觉对小肠运动的调节中还有体液调节的参与。

Result] The extracted liquid from D. stramonium could decrease the germination rates of 4 vegetables and the seed germination of tomato and pepper. After treatment by 0.2, 0.4 g/ml extracted liquid , the seed germination of tomato and pepper were decreased by 10.2%, 9.4% and 46.7%, 34.7% than that of CK resp. The extracted liquid at 0.2 g/ml could inhibit the growth of vegetable root, but had some promotion on growth of vegetable stem. The extracted liquid at 0.4 g/ml had the strong inhibition on the growth of vegetable root, but its inhibition on growth of vegetable stem was greatly variable in different species.

结果]曼陀罗浸提液可降低4种蔬菜种子的发芽速率,降低番茄和辣椒种子的发芽率;0.2、0.4 g/ml曼陀罗浸提液处理后,番茄和辣椒种子的发芽率分别比对照降低了10.2%、9.4%和46.7%、34.7%。0.2 g/ml曼陀罗浸提液抑制蔬菜根的生长,而对茎的生长却表现出促进作用;0.4 g/ml曼陀罗浸提液对蔬菜根的生长有很强的抑制作用,对茎段生长的抑制作用因物种不同而有很大差异。

The suboptimal thermoperiod was 15℃/25℃. 2. The fresh H. ammodendron seeds had highly germination ability, and have no dormant phenomenon; The fruiting wings had significant inhibition the germination of fresh mature seed in autumn, and fruiting wings made H. ammodendron seeds maintain dormancy, but with store time extend, the effect that fruiting wings inhibited seed germination gradually alleviate, fruiting wings didn't inhibition effect of seed germination until the next spring(in 4 month).

2梭梭新采收的去翅种子不存在休眠现象,而具有很高的萌发生活力;果翅对秋天新成熟种子的萌发有显著的抑制作用,使种子处于强迫休眠状态;果翅对种子萌发的抑制作用随着贮藏时间的推移逐渐降低,到翌年春天(4月)果翅解除对种子萌发的抑制作用。

Persicum seeds was 5℃/15℃. The suboptimal thermoperiod was 15℃/25℃. 2. The fresh H. ammodendron seeds had highly germination ability, and have no dormant phenomenon; The fruiting wings had significant inhibition the germination of fresh mature seed in autumn, and fruiting wings made H. ammodendron seeds maintain dormancy, but with store time extend, the effect that fruiting wings inhibited seed germination gradually alleviate, fruiting wings didn't inhibition effect of seed germination until the next spring(in 4 month).

2梭梭新采收的去翅种子不存在休眠现象,而具有很高的萌发生活力;果翅对秋天新成熟种子的萌发有显著的抑制作用,使种子处于强迫休眠状态;果翅对种子萌发的抑制作用随着贮藏时间的推移逐渐降低,到翌年春天(4月)果翅解除对种子萌发的抑制作用。

Results are as followed:1 Exposure of HELF cells to BP caused c-Jun activation,and increased the activity of MAPK,PI-3K,p53 and cyclin D1 pathway.2 BP-induced c-Jun activation was inhibited by dominant negative mutants of extracellular signal-regulated protein kinase or c-Jun NH_2-terminal kinase,but not by p38,impling that JNK and ERK pathways medicate c-Jun activation induced by BP.3 Overexpression of dominant-negative mutants PI-3K and Akt potently blocked phosphorylations of c-Jun and ERK,but not JNK in response to BP,suggesting that PI-3K/Akt pathway positively regulates BP-induced c-Jun activation through ERK.4 Inhibition of p53 by its chemical or molecular inhibitor markedly increased the phosphorylation levels of c-Jun,Akt and ERK upon BP stimulation,indicating that p53 negatively medicates BP-induced c-Jun activation through PI-3K/Akt/ERK pathway.5 The cell lines expressed TAM67 exhibits no significant affecting normal cell growth properties.6 TAM67 was able to significantly block G_1-S transition and subsequent cell proliferation,suggesting that c-Jun is essential for cell cycle alternations elicited by BP.7 Overexpression of TAM67 impaired BP-induced cyclin D1 activation,decreasing expression of E2F1 and pRb,indicating that c-Jun participates in the modulation of BP-induced activation of cyclin D1/pRb/E2F1 pathway.8 Stably expression of TAM67 led to the increases in the expression levels of p53 and p21,elevating phosphorylation level of p53,clearly indicating that c-Jun regulates p53/p21 pathway activation induced by BRCollectively,PI3K/Akt/ERK pathway mediated BP-induced c-Jun activation through p53-dependent mechanism.

结果显示:1BP刺激细胞可促进c-Jun活化,并伴随着MAPK、PI-3K、p53和cyclinD1通路各组成成分的活性增强。2利用MAPK通路的显性失活突变体分别阻断细胞外信号调节激酶和c-Jun氨基末端激酶活性,均可明显抑制BP诱导的c-Jun活化,但阻断p38活性对BP引起的c-Jun活化无明显影响,提示JNK和ERK通路参与调控BP诱导的c-Jun活化。3过表达PI-3K和Akt的显性失活突变体也可显著抑制BP诱导的c-Jun活化,并降低磷酸化ERK的表达水平,但对磷酸化JNK的表达水平无明显影响,说明PI-3K/Akt通路通过ERK正性调控了BP诱导的c-Jun活化。4p53的化学/分子抑制剂能使BP作用的细胞内c-Jun活性明显增加,并同时诱导Akt和ERK的磷酸化水平的升高,表明p53可通过PI-3K/Akt/ERK通路对BP诱导的c-Jun活化进行负性调控。5随后观察转染细胞的生长情况,发现TAM67对细胞正常生长和形态无明显影响。6稳定表达TAM67可有效抑制BP诱导的S期细胞数的增加,提示c-Jun在BP致细胞周期改变的过程中发挥了重要作用。7TAM67过表达能够抑制BP诱导的cyclin D1活化,降低磷酸化Rb以及E2F1蛋白表达水平,表明c-Jun参与调控BP诱导的cyclin D1/Rb/E2F1通路的活化。8过表达TAM67可使BP刺激的细胞中p53、p21总蛋白以及p53磷酸化的表达水平明显升高,可见c-Jun也参与调控BP诱导的p53/p21通路活化。

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I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时,滚道或滚动元件褪色是很常见的特征。

The lawyer's case blew up because he had no proof.

律师的辩护失败,因为他没有证据。