抑制...

Studied on sensitivities of C. ellipsoidea to kanamycin , Chloromycetin , Hygromycin , Streptomycin and Geneticin (G418), the results showed that C. ellipsoidea is not sensitive to Km, Str and Cm, 600μg/ml km, 600μg/ml Str and 200μg/ml Cm can not inhabited the growth of C. ellipsoidea. However, it is fairly sensitive to Hm, 100μg/ml can completely stop the growth of C. ellipsoidea on solid media. We also found that C. ellipsoidea was highly sensitive to G418, 30μg/ml and 15μg/ml can inhabit the growth of C.

通过5种基因工程中常用抗生素对小球藻生长抑制的研究发现：小球藻对卡那霉素、链霉素、氯霉素不敏感。600μg/ml的卡那霉素、600μg/ml的链霉素和200μg/ml的氯霉素仍不能抑制小球藻的生长；小球藻对潮霉素较为敏感，100μg/ml的用量可抑制固体培养中小球藻的生长；而对G418表现出高度敏感性，30μg/ml的G418即可完全抑制固体培养中小球藻的生长，15μg/ml的G418即可完全抑制液体培养中小球藻的生长。

Ovoalbumin-induced rat feet edema was significantly decreased by QLS with the doses of 35 and 70.0 mg·kg-1·d-1 (P.05).Cotton pellet-induced rat granuloma was significantly decreased by QLS with the doses of 35.0 and 70.0 mg·kg-1·d-1 (P.05 or P.01 ) and the granuloma inhibitory rates were 32.40 % and 35.65 %,respectively.

结果：QLS 3个剂量组均不同程度地抑制小鼠耳肿胀度，与空白对照组比较，50及100 mg·kg-1·d-1 QLS组小鼠耳肿胀度均明显减小（P.05或P.01），对小鼠耳肿胀的抑制率分别为53.97%和60.09%；QLS 35.0及70.0 mg·kg-1·d-1组均显著抑制大鼠足肿胀（P.05），且对棉球所致肉芽组织增生有明显的抑制作用（P.05或P.01），大鼠肉芽肿的抑制率分别为32.40 %和35.65 %。

Parthenolide is extract from feverfew, it's main component is sesquiterpene, it is commanly used in west country and is mainly used to therapy skin infection, rheumatic disorder, migraine. But recent research indicate that parthenolide can regulate many pathway of signal in the cell, inhibit the cyclooxygenase, TNF-α, interleukin-1 expression induced by lipopolysaccharide in macrophage, inhibit the iNOS expression in human monocyt, inhibit the activity of NF-κB in Hela cell, inhibit the growth of tumor cell and can relieve the myocardial ischemical reperfusion injury and so on. But the invention research about the effection of parthenolide on the VSMC is very rare in the world, so we do some research about the effection of inhibition of parthenpolide on the proliferation of VSMC and the signal transduction mechanism.

小白菊内酯(parthenolide，又名Sesquiterpene lactone)是艾叶菊属黑叶母菊的提取物，主要成分是倍半萜烯内酯，西方国家较为常用，主要用于治疗皮肤感染、风湿病和偏头痛，但近年来发现小白菊内酯能调控细胞内多种信号途径，如抑制巨噬细胞中脂多糖诱导的Cox，肿瘤坏死因子α和白细胞介素-1的表达，能抑制人单核细胞中iNOS的表达，能抑制Hela细胞中NF-κB的活性，抑制肿瘤细胞的生长，同时能减轻心肌缺血再灌注损伤等，但小白菊内酯在VSMC中的干预研究目前国内外非常罕见，为了确切评价小白菊内酯对血管平滑肌细胞增殖的抑制作用及相关的信号转导机制，我们进行了一些研究。

It is the fluorosilicic ion that exsits in the slurry and plays an important role in flotation, fluorosilicic ion not only reacted with Fe〓 on the surface of aegirine to form ionic compound, but also formed stable Si-F bond in Si-O etching reaction. Only ionic compound was obtained when it was attached to hematite surface according to the theories of coordination chemistry, Compared with SiF〓, Fatty acid ion preferentially acts with Fe〓 on the minerals surface, therefore, SiF〓 Can't obstruct the adsorption of collector on the surface of hematite completly, but can reduce the flotation recovery of aegirine selectively.

运用FTIR、XPS等测试方法仔细研究了氟硅酸盐抑制剂对含铁硅酸盐钠辉石的选择性抑制作用机理，氟硅酸盐在弱酸性条件下对赤铁矿浮选的抑制作用很微弱，而对含铁硅酸盐钠辉石强烈抑制，从而扩大了两者之间的浮选差异；SiF〓是矿浆中起抑制作用的主要成分，SiF〓不仅与钠辉石表面的Fe〓形成离子型配合物，而且还与Si-O键形成稳定的Si-F共价键，而与赤铁矿表面只能形成离子型配合物，根据晶体配位场理论，与SiF〓相比较羧酸根离子将优先与矿物表面Fe〓发生配位作用，因此SiF〓的存在并不能完全阻碍捕收剂在赤铁矿表面的吸附作用，却能够选择性地抑制钠辉石的上浮。

These studies indicate the rationality of our molecular design based on the crystal structure of KARI complex. To obtain more KARI inhibitors with high activities, the molecular docking and 3D-QSAR methods were applied to our further studying of these amidine compounds. Two methods AutoDock3. 0.3 and DOCK4. 0 were used in molecular docking, and the calculate results accord with that of herbicidal tests in general. The CoMFA model from 3D-QSAR analysis provided useful guidance for designing highly active compounds prior to their synthesis.

这些分子作为潜在的KARI抑制剂为基于KARI的农药分子设计提供了有利指导；对部分小分子进行了合成，在除草活性初筛的近三十个化合物中，个别化合物表现出较好的除草活性，其中一个化合物wb1596对油菜和稗草具有较高的生长抑制率，并通过E.coli KARI酶活性抑制试验验证了其KARI酶的作用靶标，此化合物可以作为KARI一个有苗头的抑制剂为后续工作奠定了基础；对发现的有苗头的KARI抑制剂进行了结构修饰和优化，合成了一系列脒类化合物并确证了它们的结构，改造后的化合物与原抑制剂相比大多除草活性相当或更佳，从而表明我们基于靶标结构的分子设计的合理可靠性；对所合成一系列脒类化合物进行了分子对接和三维定量构效关系研究。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

Wenxin capsule can promote endolethial cells releasing CO and synthesing 〓, enhance activity of HO, induce angiectasis through activating guanylic cyclase and adenglate cyclate of intracellar of vessel smooth muscle cell to enhance content of intracellar cGMP and AMP. 3. Wenxin capsule can inhibit releasing of platelet activiting factor 〓 which inhibit platelet aggregation and have antithrombosis function. 4. Wenxin capsule can promote activity of t-PA, reduce activity of PAI-1, strengthen fibrinolytic function, therefore inhibit fibrin disposition. 5. Wenxin capsule can inhibit synthesis of smooth muscle cellular DNA. Its function don't kill it directly but prevent transformation from period Go to peried S. 6. Wenxin capsule is an effective prescription of anti-coronary atherosclerosis atherosis. The results of image pattern analysis tastifies that area of lipid plaque in treating group reduce 54% and 58% in contrast with that in control group. The results of light, electro- microscopy show that structure of endolethial cell and smooth muscle cell in treating group is more integral than that in control group.

其作用机制之一是由于该药具有良好的调脂降脂作用，其不仅能明显降低血清LDL-c及升高HDL-c含量，且能有效阻止TC沉积于血管壁，从而保护内皮细胞结构完整；此外，温心胶囊尚具有明显的抗氧化作用，该药不仅能明显降低血浆LPO含量，且能提高SOD活性，同时其对血红素在细胞内代谢的调节作用也参与了抗氧化过程。2 温心胶囊能明显促进内皮细胞释放CO及合成〓，提高HO活性，通过激活血管平滑肌细胞内鸟苷酸环化酶及腺苷酸环化酶引起细胞内cGMP、AMP浓度增加而引起血管舒张。3 温心胶囊能明显抑制血小板激活因子〓的释放，从而抑制血小板聚集，抗血栓形成。4 温心胶囊能明显提高t-PA活性，降低PAI-1活性，增强纤溶功能，从而抑制纤维蛋白沉积。5 温心胶囊能明显抑制平滑肌细胞DNA合成，其抑制作用不是直接杀伤抑制，而是阻止细胞由Go期向S期转化。6 温心胶囊是抗冠状动脉粥样硬化的有效制剂，图象分析结果证实，该药治疗组脂斑面积比对照组缩小了54％和58％，光电镜结果显示内皮细胞及平滑肌细胞结构较为完整。

The fungicidal activity of methanol extract was higher than that of ethylacetate extract. The bioassay showed that the methanol extracts of strain As fermentation products had certain degree fungicidical activity against Botrytis cinerea, Alternaria longipes, Glomerella cingulata, Curvulavia lunata, Gibberella zeae, Valsa malt under the concentration of 4000μg/mL, the inhibiting ratio against pathogen growth was ranging from 62.9%~85.1%; The results of the inhibition of spore germination indicated that the fermentation products exhibited obvious inhibition rate against Alternaria longipes, the EC50 values was 62.5328μg/mL; The bioassay showed that the methanol extracts of strain As fermentation products had certain degree fungicidical activity against Bacillus cereus , Bacillus subtilis.

其中甲醇提取物的抑菌活性较乙酸乙酯好，在4000μg/mL浓度下对番茄灰霉病菌、烟草赤星病菌、苹果炭疽病菌、玉米弯孢叫斑病菌、小麦赤霉病菌、苹果腐烂病菌6种植物病原真菌均有抑制作用，抑制率在62.9%~85.1%；抑制孢子萌发试验表明：菌丝体甲醇提取物对烟草赤星病菌的毒力较好，其EC50仅为62.5328μg/mL；抑制细菌活性表明菌丝体甲醇提取物对蜡状芽孢杆菌和枯草芽孢杆菌有一定的抑制作用，而对大肠杆菌无明显的抑制作用。

However, membrane suppressors also have certain disadvantages compared to packed bed suppressors such as cost, leakage of regenerant causing higher detector background, the requirement for an external supply of regenerant solution and the fact that membrane suppressors are complex and not user serviceable.

&然而，与填充床抑制器相比，膜抑制器也有不足之处，如成本，再生剂泄漏导致更高的检测器背景，需要外部提供再生溶液，以及膜抑制器的复杂性和用户难以操作的事实等等，因此，开发低价、高性能填充床抑制器会更有益。&这正是万通致力所为！万通MSM抑制器提供十年的保用期，是现有的最成熟、尖端的填充床抑制器。

The inhibition substances in Sorbus pohuashanensisHedl fruit not only restrained the seeds of wheat, cabbage from germinating but also restrained height and root growth. After the seeds of wheat, chinese cabbage had been soaked in the water extraction of pulp, seed and decoated seed for 8h, the seed germination rates were 92% and 84%, 89% and 65%, 94% and 78%, lower than the control, especially the water extract of decoated seed; and the root length and seedlings height were lower than the control, especially the water extract of pulp, and decreased in sequence of soaking time from short to long. So the simplest and effective method to eliminate pulp soluble inhibiting substances is to wash fruits by flowing tap water soon after collection.

在几种浸泡时间中，浸泡8小时的去皮种子浸泡液对白菜、小麦种子发芽率的抑制作用最为明显，其发芽率分别为65%、89%，但随着浸泡时间的延长而降低；浸泡8h的果肉浸泡液对白菜和小麦幼苗高生长和根伸长的抑制作用最为明显，种子和去皮种子浸泡液(8h)的抑制作用对小麦和白菜的抑制作用次之，随着浸泡时间的延长，果肉浸泡液的抑制作用逐渐减弱，因而采收下来的果实及时净种是清除果肉水溶性抑制物质简单有效的方法。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。