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The HE activity was almost completely inhibited by SBTI, p-APMSF, bestatin and NEM, greatly inhibited by ovomucoid, TLCK, IAM, chymostatin and PMSF, and slightly inhibited by pepstatin A, TPCK, LBTI and leupeptin. And these results imply that HE is most probably a trypsin-type serine protease.

抑制剂对其酶活性的影响结果显示,该孵化酶的活性几乎可被SBTI、p-APMSF、Bestatin和NEM所完全抑制,被ovomucoid、TLCK、IAM、chymostatin和PMSF所强烈抑制,而对pepstatin A、TPCK、LBTI和leupeptin不敏感,表明该酶极可能是一种属于胰蛋白酶类型的丝氨酸蛋白酶。

Application of EBR induced parthenocarpic growth accompanied with active cell division in Jinchun No.4, a cultivar without parthenocarpic capacity, whereas Brz treatment inhibited fruit set and subsequently fruit growth in Jinchun No.2, a cultivar with natural parthenocarpic capacity, and this inhibitory effect could be rescued by application of EBR.

在非单性结实的津春4号黄瓜栽培品种的子房上添加EBR可以诱导单性结实并且提高细胞分裂能力,而在单性结实的津春2号黄瓜栽培品种的子房上喷施BRs合成抑制剂抑制果实生长,最终导致败育,而这种抑制现象可以通过添加EBR得以恢复。

Suppressor is the key part for IC instrument, and developing a high performance suppressor for IC instrument is quite important to gradient ion chromatography, to analysis of some ions which have strong affinities with the functional groups located on the surface of the pellicular ion exchange column packing.

抑制柱是离子色谱仪的一个核心部件,研制具有较高抑制容量的抑制柱对于离子色谱法的梯度淋洗、对于在分离柱上保留时间长的离子分析具有重要的意义。

The purpose of this study is to screen microorganisms with growth inhibition against plant fungal pathogens. The plate-dilution method was used to isolate the bacteria from salt marshes at Dafeng, Jiangsu. The antifungal activity of the bacterial broth filtrates against 5 phytopathogens was determined using mycelial disk method. The antifungal compositions of four active bacteria were analyzed primarily with chromatography techniques. Two hundred and thirty-five aerobic (92) and anaerobic (143) bacterial strains have been isolated from the coast salt marsh and sixty-five strains, of which 39 are aerobic and 26 are anaerobic showed greater than 30% of inhibition rate against the growth of at least one of the phytopathogenic fungi on petri dish.

为了开发对植物病原真菌生长有抑制作用的药源微生物,本实验采用平板稀释法从江苏大丰盐沼湿地土壤中分离微生物,并通过菌丝块法检测这些微生物代谢产物对5种植物病原真菌生长的抑制活性,进而利用柱层析分析其活性成分;结果共分离得到235株细菌,其中好氧细菌92株,厌氧细菌143株;体外活性测试结果显示有65株至少对1种测试植物病原真菌的抑制率在30%以上,其中好氧菌39株,厌氧菌26株。

Methods Spectrophotometry was used to study the activity of PLAP in the presence and absence of L Leu. PLAP relative activity was calculated according to the absorbance variation.

方法应用分光光度法分别测定有L-Leu和无L-Leu存在下的酶活力,根据其活力变化求出酶的相对活力;用双倒数作图法,确定L-Leu对PLAP的抑制类型并求出其抑制常数;同时也观察了pH对抑制作用的影响。

The result of polyhydroxy benzamides for whitening, the activity evaluation of tyrosinase inhibitory, it has apparently effect on the caffeic acid, gallic acid and 2, 5-dihydroxybenzoic acid of amide derivatives (IC50 = 62.41~130.28 μg/ml), but some of the compounds show apparently the activity of tyrosinase inhibitory,(ex:IC50 = 71.70 μg/ml of compound 12 and 57.26 μg/ml of compound 24) inhibitory efficacy.

多酚醯胺化合物在美白活性结果,抑制酪胺酸酶活性评估,以咖啡酸、没食子酸及2, 5-二羟基苯甲酸之醯胺衍生物效果最为显著(IC50 = 62.41~130.28 μg/ml),但也有少酗坐あX物显示出明显抑制酪胺酸酶活性能力,如(Compound 12之IC50 = 71.70 μg/ml、Compound 24之IC50 = 57.26 μg/ml)也出现较强之抑制酪胺酸酶活性能力。

It was demonstrated that the growth of glioma in rat can be inhibited by PCNA antisense oligonucleotides, the expression of PCNA target gene and prolification of glioma cells can be inhibited by PCNA antisense oligonucleotides.

PCNA反义寡核苷酸对胶质瘤的生长具有抑制作用,通过反义技术可抑制靶基因的表达,从而抑制胶质瘤细胞的增殖。

And found root exudates have the promotable effect on the activity of IAAoxidase of cucumber and squash,.

在对其生理生化特性的研究中发现,根系分泌物显著的促进黄瓜和南瓜体内吲哚乙酸氧化酶的活性,从而降低其体内吲哚乙酸的水平,抑制其生长发育;根系分泌物还显著抑制受体淀粉酶的活性,这种抑制性随浓度升高而增加,通过影响黄瓜和南瓜体内的能量转化来影响其正常生长。

Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果:经槲皮素处理的人结肠癌SW480细胞数量减少,部分细胞体积缩小,细胞膜完整,胞浆浓缩,核染色质固缩,细胞核碎裂,形成凋亡小体;MTT法检测显示当作用浓度为30μmol/L~90μmol/L时,槲皮素对人结肠癌SW480细胞的生长有抑制作用,其抑制作用随着作用浓度的增加和作用时间的延长而增强;流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期,大部分细胞被阻断于S期,随药物浓度的升高和作用时间的延长,G0/G1期细胞比例逐渐增加,S期细胞比例逐渐减少;酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9,随浓度的升高,MMP-2及MMP-9的分泌量减少;免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后,Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

The results showed that some strains had effect on the Pythium aphanidermatum, but no effect on Fusarium oxysporum. Seven bacterial strains were obtained which could suppress the hatch of H. glycines.

结果表明,部分菌株对大豆根腐病原菌-瓜果腐霉菌抑制效果明显,但对尖孢镰刀菌抑制效果不明显;获得了7株对大豆胞囊线虫胞囊孵化具有抑制效果的菌株。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。