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Result: The nm-HAP-sol present the dispersed and uniformity needle. A431cells inhibition ratio was maximizing at the 5 day in the 400mg/L nm-HAP-sol group, the 200mg/L nm-HAP-sol group and the 100mg/L nm-HAP-sol group, inhibition ratio of the 400mg/L nm-HAP-sol group, the positive control group and the 50mg/L nm-HAP-sol group was respectively (77.89±6.29)%,(77.46±8.26)% and (1.23±0.15)%. It was detected that the number of A431 cells were persistently increasing in the 50mg/L nm-HAP-sol groups while decreasing in the 400mg/L nm-HAP-sol group, the positive control group and abidingly increasing for 4 and 3 days then decreasing in the 100mg/L and 200mg/L nm-HAP-sol group by inverted microscope.

结果:超声乳化法配制的HAP纳米溶胶呈均匀分散的针状;400mg/L、200mg/L和100mg/L溶胶组对A431细胞抑制率在第5天达到各组最大值,此时400mg/L溶胶组与阳性对照组的抑制率分别为(77.89±6.29)%和(77.46±8.26)%, 50mg/L溶胶组抑制率为(1.23±0.15)%;倒置显微镜下见50mg/L溶胶组细胞数目持续增加,聚集成簇,细胞呈现多角形或梭形,胞浆透亮,而100mg/L 和200mg/L溶胶组的细胞数目分别增加至第4天和第3天开始下降,400mg/L溶胶组和阳性对照组细胞数目持续减少,细胞体积缩小,漂浮细胞增多。

It,s allelchemicals were isolated through extraction, thin-layer chromatography, and column chromatograpathy. After the white clover was infused in ethanol and evaporation, the leavings was dissolved by water. The aqueous extract was extracted by petroleum,chloroform, ethyl acetate and n-Butanol successively, and the bioassay showed that the chloroform phase has obvious inhibitory effect, the inhibitory rate was 44.32%,88.08% on Abutilon theophrasti M., seedling length and root length, 82.08%,92.16% on Echinochloa crusgalli L., seedling length and root length, and the Amaranthus retroflexux L., germination rate is 0, the secondly was ethyl acetate phase.

运用萃取、薄层层析、柱层析等方法对白三叶草地上部分的化感物质进行初步分离,结果表明,白三叶草经乙醇浸提旋转蒸发后用蒸馏水溶解,依次用石油醚、氯仿、乙酸乙酯、正丁醇萃取,生物测定表明氯仿组分对杂草的抑制作用最强,对苘麻芽长和根长抑制率分别为44.32%、88.08%,对稗草芽长和根长的抑制率分别为82.08%、92.16%,反枝苋的发芽率为0;其次是乙酸乙酯相。

The results showed that among the 3 NO donors,only GSNO had significant effects on the percentage of sporulated oocysts,theinhibiting rate of purified sporulated oocysts was 93% to 97%,and reached 100% onthe oocysts in the faces of chicken with E.tenella infection.GSNO also significantlydecreased virulence of E.tenella oocysts;and the oocysts treated by 2~8 mmol/LGSNO almost lost their pathogenicity to chickens.

试验结果表明,在以上3种NO供体中,只有GSNO对卵囊的孢子化率有明显的抑制作用,其对纯化卵囊的抑制率为93%~97%,对粪便中卵囊的抑制率可达100%;GSNO还能明显抑制E.tenella卵囊的毒力,经2~8mmol/L浓度GSNO处理的卵囊几乎丧失了对雏鸡的致病力。

The results showed that the activities of extracellular pectin methyltrans-eliminase, pectinase, extracellular C1 and proteinase were activated by asarum essential oils; the activities of pectin methylgalactuionase and intracellular C1 were activated at first, then inhibited and activated again; the activities of intracellular pectin methyltrans-eliminase were mostly inhibited; the activities of Cx were entirely inhibited by asarum essential oils, the percentage of inhibition was in proportion to the asarum essential oil concentration.

结果表明:辽细辛精油对胞外PMTE酶、果胶总酶、胞外C1酶和蛋白酶表现为激活作用,对PMG酶和胞内C1酶则表现为先激活后抑制再激活作用,对胞内PMTE酶主要表现为抑制作用;对Cx酶表现为完全的抑制作用,且酶活抑制率与精油浓度成正比。

The extracts of water and alcohol were separated by ion exchange resin. The eluates of neutral solution were the most effective against hydroxyl radicals and the highest rate of inhibiting hydroxyl radicals was 81%. The eluates of basic solution were also effective, the inhibit rate of which was 60%.

将发酵灵芝粉的水和醇提取物上离子交换树脂柱,依次用酸性、中性、碱性淋洗液洗脱分组,发现中性洗脱部分对羟基自由基抑制作用最强,抑制率最高可达81%;碱性洗脱部分亦具有抑制羟基自由基的效果;酸性洗脱部分抑制羟基自由基的作用不强,且占很少比例。

The balanced bilingualism's conflicts control ability are better than the unbalanced bilingualisms' at levels of response activation in the interference condition of Chinese color words; the balanced bilingualism and the unbalanced bilingualism have the same conflicts control ability at the levels of perceptual processing in the interference condition of Chinese color words; The balanced bilingualism's conflicts control ability are better than the unbalanced bilingualisms' at levels of perceptual processing in the interference condition of English color words; the balanced bilingualism and the unbalanced bilingualism have the same conflicts control ability at the levels of response activation in the interference condition of English color words.

在中文色词干扰条件下,在心理加工的知觉阶段,熟练双语者和不熟练双语者的抑制控制能力没有差异;在心理加工的反应阶段,熟练双语者的双语抑制控制能力明显强于不熟练双语者;在英文色词干扰条件下,在心理加工的知觉阶段,熟练双语者的双语抑制控制能力明显强于不熟练双语者;在心理加工的反应阶段,熟练双语者和不熟练双语者的抑制控制能力没有差异。

Baicalin could inhibit the initial-period and mid-period activation of mouse T lymphocytes stimulated by Con A or PDB,which suggests that baicalin have the inhibitory effect on signaling pathway of T lymphocytes activation and be a potential effective immunoinhibitory agent.

结论黄芩苷能够明显抑制多克隆刺激剂Con A或PDB诱导的T细胞早期和中期活化,且呈明显的量效关系。提示黄芩苷在T细胞活化过程的信号转导中有抑制作用,从而可以通过抑制T淋巴细胞的活化,发挥免疫抑制作用,是一种潜在的免疫抑制剂。

TCM may modulate the glial cells through the following mechanisms: inhibiting expression of iNOS, thereby minishing the toxicity of NO and its oxygen free radicals; inhibiting overactivation of astrocytes and overexpression of GFAP in acute cerebral ischemia and cerebral hemorrhage; promoting glial cells' secretion of NGF which can be caused by ischemic injury; inhibiting the nervous system's immune inflammatory response; and so on.

中药调节神经胶质细胞活性的机制可能包括:(1)抑制iNOS表达,从而抑制NO及其产生的氧自由基的毒性;(2 抑制脑缺血和脑出血急性期星形胶质细胞的过度活化和GFAP的过度表达;(3)上调缺血损伤导致的神经胶质细胞分泌NGF;(4)抑制神经系统的免疫炎症反应等各种途径。

MCP could effectively delay the softening of climacteric fruit, such as Starking and Fuji apples and Feicheng peach, but as different fruits had different softening mechanism, the mechanism of 1-MCP inhibiting different fruit softening was different: in Starking apples, the key enzyme to the softening is endo-and exo-PG, while during Feicheng peach fruit softening, the key enzyme was EGase before the 20th d and endo-PG after 20th d during storage at 0℃. 1-MCP inhibited fruit softening mainly by inhibiting PG activity in Starking apple while in Feicheng peach, by inhibiting the activity of EGase in the earlier storage time and endo-PG in the latter time. It was also found that LOX accelerated the initiation of the softening and was regulated by ethylene and 1-MCP in apple and peach.

2.1-MCP能较好的延缓红星、富士和肥城桃等跃变型果实硬度的下降,但是不同品种/树种的果实软化机理不同,1-MCP抑制其软化的机理也不一致:在红星果实中,对软化起主要作用的酶一直为内切和外切两种PG,而在肥城桃软化过程中,前20d以纤维素酶活性起主要作用,20d后软化以内切PG为主。1-MCP主要通过抑制红星果实中PG酶活性来抑制果实硬度的下降;在肥城桃果实中,1-MCP通过抑制前期纤维素酶和后期内切PG活性,延缓了果实硬度的下降。

Results were shown as followings:(1) Sodium selenite at 0~2.5 μmol/L significantly increased the antioxidative capacity of L-02 cells without having remarkable impact on SMMC-7721 cells;(2) Sodium selenite at concentrations above significantly increased telomerase activity, hTERT gene expression and telomere length of L-02 cells without significant impact on SMMC-7721 cells;(3) Sodium selenite at higher concentrations (larger than 5 μmol/L) resulted in peroxidation of L-02 cells, while scutellarin significantly counteracted its effect;(4) Selenium-rich amino acids from silkworm pupas in the range of 0.5~2.5 μmol/L Se significantly inhibited SMMC-7721 cell growth, induced apoptosis and cell cycle change, and the generation of reactive oxygen species. In contrast, sodium selenite and selenomethionine only had weak impact on them at the same concentrations;(5) A new selenium-containing protein was found from selenium-rich silkworm pupas, which is worthy to be study further;(6) An expression vector containing ansense RNA of hTERT gene were constructed and used to transfect SMMC-7721 cells. They were observed to inhibit hepatoma cells.

结果如下:(1)0~2.5μmol/L亚硒酸钠显著性增强L-02细胞的抗氧化能力;而对SMMC-7721细胞的作用不显著;(2)该浓度硒显著性提高L-02细胞端粒酶活性、增强hTERT基因表达和延长细胞端粒长度;而对SMMC-7721细胞的作用均不显著;(3)高浓度硒(5μmol/L以上)显著性抑制L-02细胞生长、致细胞过氧化,灯盏花素能拮抗硒所致过氧化、降低硒毒性;(4)0.5~2.5μmol/L富硒蚕蛹氨基酸显著性抑制肝癌细胞SMMC-7721生长、导致细胞凋亡和周期改变、诱导细胞产生活性氧,同浓度亚硒酸钠和硒代蛋氨酸对其抑制不显著;(5)富硒蚕蛹蛋白经分离纯化和鉴定后发现存在一新含硒蛋白,其结构和功能有待研究;(6)通过已有的含hTERT基因质粒,成功构建hTERT反义RNA表达质粒,转染SMMC-7721细胞后对其生长具有抑制作用。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。