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In both Pacific oyster and Zhikong scallop, gonad of triploid animal was observed to development in the developing seasons. While during the gonadial developmental stage since 3 to 6 year-old, gonads of all triploid Pacific abalones fail to develop. Whether there is relation between a-TSP and gonadial development is to be studied.

皱纹盘鲍的三倍体个体性腺基本不发育,而长牡蛎和栉孔扇贝的三倍体虽然性腺发育不良,但可产生成熟的配子并可育,因此a-TSP与鲍的性腺发育抑制可能有关,但尚有待证实。

This is a culture by the Chinese aquaculture industry milestone insurance first big single, the scope of protection is theirIsland fisheries waters of the six breeding Patinopecten yessoensis, Haliotis discus hannai, gill, such as sea cucumbers and sea urchins four breeding Seafood .

这是中国水产增养殖行业具有里程碑意义的第一笔保险大单,保障的范围是獐子岛渔业所属的6个养殖海域的虾夷扇贝、皱纹盘鲍、刺海参和海胆等4个养殖海珍品。

Steamed scallop shumai with tobiko caviar are fresh and meltingly soft.

扇贝舒脉与tobiko鱼子酱是新鲜和meltingly软。

Chlamys farreri ; Acute virus necrobiotic disease virus; ELISA ; Electron microscopic observation

栉孔扇贝;急性病毒性坏死症病毒; ELISA ;电子显微镜观察

The acute virus necrobiotic disease virusC AVND viruswas isolated from diseased scallop Chlamys farreri collected in the Taiping Bay of Qingdao. Using purified virus as antigens, the polyclonal antibody with high titer was prepared in rabbits. The polyclonal antibody as the first antibody, IgG-HRP as the second antibody, indirect ELISA technique was set up through selecting the best interactional conditions among the first antibody, the second antibody and antigen.

以患病栉孔扇贝组织中提纯的急性病毒性坏死症病毒作为免疫抗原,注射新西兰白兔制备出效价高达1:32000的多克隆抗体作为第一抗体,第二抗体为辣根过氧化物酶标记的羊抗兔IgG,通过交叉稀释法确定抗原、一抗及二抗的最优反应条件,建立酶联免疫吸附分析方法体系,病毒检测灵敏度达30ng。

The distribution of acute virus necrobiotic disease virus in mantle, gill, hepatopancreas, kidney, intestine and muscle of scallop Chlamys farreri naturally diseased and artificially infected was detected by ultra thin section electron microscopic technique, negative staining electron microscopic technique and ELISA.

应用超薄切片、负染电镜技术以及ELISA对自然发病和人工感染发病的栉孔扇贝之外套膜、鳃、肝胰腺、肾、肠、及闭壳肌等主要器官急性病毒性坏死症(AcuteVirusNecrobioticDisease,AVND)病毒的感染状况进行了研究。

To 26. 23, P <0. 01.CONCLUSION: Chlamys farreri is capable of increasing Bcl-2 protein and decreasing Bax protein in cerebral penumbra to brake the initiation of neuron apoptosis after ischemia-reperfusion and preserve neuronic function in penumbra.

扇贝多肽能够上调脑缺血半影区内抑制神经细胞凋亡的Bcl-2蛋白水平,同时降低半影区内诱导神经细胞凋亡的Bax蛋白的表达,从而在阻止脑缺血再灌注后细胞凋亡启动中起制动作用,保护脑缺血后半影区内神经元的功能。

In order to obtain viable tetraploids, which remains a major challenge worldwide, research on spindle organization and chromosome behavior was carried out mainly in zhikong scallop, Chlamys farreri, a typical commercially important species which is being cultured in North China Sea. Immunofluorescent technique and laser scanning confocal microscopy has been developed for observation of both microtubule organization and chromosome segregation. Orcein squash method was also used for chromosome observation. A series of complicated configurations of the meiotic spindle and associated patterns of chromosome segregation, including tripolar and seperated bipolar segregation, were found in developing eggs following the inhibition of the first polar body.

为了攻克可存活四倍体人工培育技术的国际性研究难题,本研究选取了我国北方海区的典型经济贝类栉孔扇贝为主要研究对象,建立了一套免疫荧光染色和激光扫描共聚焦显微技术,结合使用染色体地衣红压片观察技术,对第一极体受抑制的受精卵的减数分裂纺锤体装配、染色体分离动态进行了研究,发现了一系列特殊的纺锤体装配及相应的染色体分离行为,包括三极分离、独立二极分离等分离类型,从亚细胞水平上阐明了四倍体形成的细胞学机理。

Three kinds of typical shells, such as scallop, whelk, bloody clam, as well as one kind of limestone were chosen to perform desulfurization and calcination experiments on the fluidized bed platform.The microstructures of the tested samples were mensurated by means of one mercury porosimeter.

在流化床上对3种典型贝壳---扇贝、海螺、毛蚶以及一种石灰石进行煅烧脱硫试验研究,并利用压汞仪测定试验样品的微观孔隙结构,通过最可几孔径、孔隙率、比表面积等3个结构特征参数对微观结构进行定量分析;另外,还对试验样品的化学成分进行了分析。

And MSAP with 24 primer was used for analysis in Yesso scallops with orange/white muscle, it was showed that there were 46 loci which had differences in the degree of methylation between the population with orange muscle and the white, among of which 18 were methylated or semi-methlated loci, which may be related to the appearance of orange muscle.

采用24对引物组合对橘红/白色闭壳肌虾夷扇贝群体进行了MSAP分析,结果表明,甲基化程度存在差异的位点46个,其中18个位点为甲基化或半甲基化位点,推测橘红色闭壳肌的发生可能与这些位点有关,它们调控了该性状的表达。

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