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When C.farreri×P.yessoensiswere tested by using6pairs of microsatellite primers which could be applied to boththe two kinds of scallops,the specific bands could be amplified fromeachone of the 6 pairs,among which,the specific bands fromboth agnate and maternal colony could be distinguished obviouslyinthe amplified products of P13F449 and KMY134,and accordinglythe hybrid identity of hybrid generations could be affirmed.

将这6对具有属间通用性的微卫星引物对栉孔扇贝×虾夷扇贝进行PCR扩增,均能扩增出特异性条带,其中引物P13F449和KMY134的扩增产物中可以明显地分辨出来自父母本群体的特有条带,从而确定了杂交子代的杂种身份。

There was also not any haploid metaphases (n=16 or 19) was detected in hybrids. However, about 1-2% metaphases observed in this study was allopolyploid. GISH proved that they was made up of haploid genomes of M. nobilis (n=16) and triploid genomes of C. farreri (n=57). GISH was also employed to detect different chromosome components and identify donor chromatin in hybrids between C. farreri♀×A.

但我们检测到了约1-2%异源多倍体的分裂相,GISH分析后发现这些分裂相是由单倍的华贵栉孔扇贝的染色体和多倍的栉孔扇贝染色体组成,比如论文中图示了一个异源四倍体的分裂相,它是由单倍的华贵栉孔扇贝的染色体(n=16)和三倍的栉孔扇贝染色体(3n=57)组成。

The in vitro phagocytosis property of scallop Chlamys farreri and ark shell Scapharca subcrenata were studied here.

实验发现,在自然状态下,栉孔扇贝有能够检测到的活性氧产生,本实验检测的栉孔扇贝的NBT阳性细胞占血细胞总数的20一35%。

The study aimed to provide the base for hybrid identificationof Patinopecten yessoensis×Chlamys f arreri population and paternity testing in family in great scale.

为进行大规模栉孔扇贝×虾夷扇贝群体的杂种鉴定以及家系中的亲子鉴定提供了基础。

Virus and RLO were detected in tissues of Chlamys farreri and infection rate of virus ranged from 80% to 100% during cultured period. The infection intensity of virus varied evidently and the highest infection intensity of virus was found in July and August when acute death of Chlamys farreri occurred.

而栉孔扇贝在整个养殖期间,病毒感染率基本保持在80%-100%之间,感染强度在不同养殖季节变化极为明显,其中感染强度的高峰出现在栉孔扇贝大规模死亡的7、8月份。

Nuclear changes in gynogenetic eggs of the scallop were examined under a fluorescence microscope during meiosis and fertilization, indicating that UV-irradiated sperm nucleus did not participate in the karyokinesis at anaphase of the first cleavage.

运用荧光显微镜观察探讨了栉孔扇贝雌核发育卵子在受精和成熟分裂过程中的核相变化,发现UV照射精核不参与核分裂,首次提供了栉孔扇贝雌核发育的细胞学证据。

Type genus of the family Pectinidae: sea and bay scallops.

扇贝科的模式属;海洋扇贝和海湾扇贝

Analysis of the secondary structure of ITS1 in Pectinidae: implications for phylogenetic reconstruction and structural evolution It is at present difficult to accurately position gaps in sequence alignment and to determine substructural homology in structure alignment when reconstructing phylogenies based on highly divergent sequences.

4栉孔扇贝扇贝科其它7种扇贝ITS1二级结构的系统进化与结构进化分析目前,在序列比对过程中对缺口进行精确定位十分困难。

It was showed that pectenolone and pectenoxanthin were all found in Gonads, hepatopancreas, mantle and gill, among of them, ovary, hepatopancreas and gill possessed more carotenoid than other tissues, but no carotenoids were found in the white muscles of Yesso scallops. Hepatopancreas contains a variety other carotenoids some kind of other not identified beside pectenolone and pectenoxanthin. The result also showed that the Yesso scallop with orange muscle had more the carotenoids in a variety of tissues than the common, and the female more than the male.4 Impacts of carotenoid accumulation on morphological structure and chemical composition in orange muscle of Yesso scallopThere was no difference between the orange and white muscle obserced by conventional HE staining, while musclular fiber type and matrix density in the orange muscle was different from white ones by the TEM observation of ultra-thin slices, and there were three musclular fiber types, among of them, the loose muscular fiber was orange muscle specifically.

利用HPLC法对橘红/白色闭壳肌虾夷扇贝各组织中含有的类胡萝卜素种类和含量,结果表明,虾夷扇贝在性腺、肝胰腺、外套膜、鳃丝中均含有类胡萝卜素pectenolone和pectenoxanthin,白色闭壳肌中未检测到类胡萝卜素,其中肝胰腺除上述两种类胡萝卜素外,还含有其他多种未鉴定的类胡萝卜素;在各组织中,雌性性腺、肝胰腺、鳃丝中类胡萝卜素的含量较高;橘红色闭壳肌虾夷扇贝各组织中的类胡萝卜素含量高于白色闭壳肌虾夷扇贝;雌性虾夷扇贝中的类胡萝卜素的含量高于雄性。4累积类胡萝卜素对虾夷扇贝闭壳肌的形态结构和化学成分的影响本文对橘红色闭壳肌进行了常规HE染色切片观察,并未观察到异常现象;超薄切片透射电镜观察发现橘红色闭壳肌在肌纤维类型及基质密度等方面有别于白色闭壳肌,在橘红色闭壳肌中有三种肌纤维,其中疏松型肌纤维为橘红色闭壳肌特有。

About four kilometers from the coast of the sea, a scallop farmers began in the platform for the work of the cage (the so-called cage for a year means that the situation in accordance with the growth of scallops, fish farmers will be to the specifications for different scallop breeding cage).

距离海岸4公里左右的海面上,一个扇贝养殖户在平台上开始了换笼工作(所谓换笼是指每年根据扇贝的成长状况,养殖户将扇贝换到规格不同的养殖笼中)。

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