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Co.,Ltd.,Tianjin 300380,China;4.Beijing Zhaoyan New Drug Research Center Co.,Ltd.,Beijing 100176,ChinaAbstract: The occurrence,migration,tissue localization and quantity changes of IgM+,IgG+ and IgA+ B lymphocytes in chicken caecal tonsil were examined using the IgM,IgG and IgA monoclonal antibodies by immunohistochemical method.

通过免疫组织化学方法,应用IgM、IgG和IgA单克隆抗体研究了IgM+、IgG+和IgA+ B淋巴细胞在鸡盲肠扁桃体中的出现、迁移、组织定位分布以及数量变化规律等一系列发育过程。

The numbersT_(ANAE~ cell number and proliferative function of T lymphocyte in caecal tonsil, the numbers of gland ofHarder of chickens treated with 5KV and 10KV HVEF were significantly higher than controlchickens; But from 1 to 35 day, them of chickens treated with 20KV HVEF, obviously were lowerthan control chickens.

盲肠扁桃体T淋巴细胞增殖功能及其数量、哈德尔氏腺淋巴细胞数量,5KV和10KV高压静电场照射雏鸡于照射后4~35天不同程度高于对照雏鸡;而20KV高压静电场照射雏鸡,于照射后1~35天均显著低于对照雏鸡。

Proliferativefunction and the numbers of B lymphocyte in caecal tonsil and gland of Harder of chickenstreated with 5KV and 10KV HVEF is more higher than these items of control chickens; But thoseof chickens exposure to 20KV HVEF is significantly lower than them of controls.

盲肠扁桃体和哈德尔氏腺B淋巴细胞增殖功能及其数量,5KV和10KV高压静电场照射雏鸡均不同程度高于对照雏鸡;而20KV高压静电场照射雏鸡则显著低于对照雏鸡。

Baby two weeks before the tonsil inflammation fever, around 38.6, intravenous drip Cephalosporium day, burning back to eat Greek inscriptions labor, cough did not improve and after three days for azithromycin Su-, three days after eating it.

孩子半个月前扁桃体发炎发烧,38.6左右,静脉点滴头孢一天,烧退后吃希刻劳,咳嗽不见好转,三天后换阿奇霉素,吃了三天后好了。

Babies from 6 months on a cold, two days to start eating like a drug, and the second drug to do to eat for a week, the 3rd on the half-eaten cold cough on drugs, the 4th doctor started me playing吊针baby bottles from 2 to 9 times hanging bottle; final Cephalosporium吊针not used, azithromycin, penicillin;(disease good will Also a week before a cold cough) are now 13 months, or so over a week on the incidence, which I also go around the middle to open Chinese medicine to the baby food; sneezing, runny nose, cough, dyspnea, tonsil inflammation of the throat red swelling, sleep a sweat, they do not release,尿黄; I now do not dare to go the hospital, and also to the baby do not know what medicine he would eat better.

孩子从6个月就感冒,开始吃了2天药就好了,第二次吃了一星期药才好,第3次就感冒咳嗽吃半个月药,第4次医生就开始给我孩子打吊针了从2瓶一次次吊到9瓶;最后头孢吊针都没用了,用阿奇霉素,青霉素;(病好了也就过一星期又感冒咳嗽)现在都13个月大了,还是这样过一星期就发病,这中间我还到处去开中药给孩子吃;打喷嚏,流鼻水,咳嗽,气急,扁桃体发炎喉咙红肿,睡觉出大汗,便干放不出,尿黄;我现在都不敢去医院了,不知道还要给孩子吃什么药他才会好。

The research isolated a strain of pantomorphic Gram-negative coccobacillus from the lung and amygdale of a pig, which has serious typical I Porcine Contagious pleuropneumonea, by use the plate of ox brain heart infusion bouillon and chocolate medium.

本研究采用以牛脑心浸液为基础的液体培养基和巧克力平板接种方法,从某猪场典型传染性胸膜肺炎症状猪的肺脏和扁桃体中分离到1株具有多形性的革兰氏阴性球杆菌。

Staining and observing shows that the most pathological changes were interstitial pneumonia and hemorrhagic pneumonia, typical nonsuppurative encephalitis, and neurogangliitis et al. The lymphoid tissue was necrosis, and the amount of lymphocytes was reduced. The pathological changes of the positive cells, especially the epithelial cells were swell, denaturization and hyperplasy to the extent.

H.E。染色观察显示,病理组织学变化主要表现为间质性肺炎和出血性肺炎、典型的非化脓性脑炎、神经节炎等;淋巴结、扁桃体和脾脏等组织内淋巴细胞坏死、数目减少;有阳性反应的细胞,特别是阳性上皮细胞呈现不同程度的肿胀、变性、增生等病理变化。

The effect of oral infection and subsequent challenge with Eimeria vaccines on the populations of CD4 , CD8 T lymphocytes in the intestine, cecal tonsils and spleen of chickens was assessed by immunohistochemistry.

用免疫组化ABC法检测鸡用上述球虫进行第一、二次免疫后小肠或盲肠、盲肠扁桃体和脾脏中CD4~、CD8~ T淋巴细胞的动态变化。

The number of CD〓 and CD〓 lymphocytes subpopulation and IgA, IgM, IgG antibody-producing cells, the ratios of CD〓 and CD〓 T-cell in the cecal tonsils and Harderian glands, the content of IgA, IgM, IgG in tear, tracheal fluid, intestinal fluid and bile of CIAV-infected chicks vaccinated with ND vaccine were significantly decreased compared with vaccinated control chicks 7~28 days PV.

CIAV感染雏鸡ND免疫后28天内,哈德尔腺、盲肠扁桃体以及泪液、气管液、肠液、胆汁的上述各项免疫学指标,均不同程度的明显低于未感染CIAV的ND免疫雏鸡,表明CIAV感染雏鸡呼吸道和消化道局部粘膜对ND疫苗的细胞和体液免疫应答机能下降。

The number of CD〓 and CD〓 lymphocytes subpopulation, ANAE〓 T cells and IgG, IgM, IgA antibody-producing cells in the local immune tissues (cecal tonsils and Harderian glands), the content of IgA, lgM, IgG in the local fluid (tear, tracheal fluid, intestinal fluid and bile) of chicks infected with CIAV were significantly decreased compared with controls 7~28 days PI.

1日龄雏鸡感染CIAV后28天内,哈德尔腺和盲肠扁桃体的ANAE〓及CD〓、CD〓T细胞和IgA、IgM、IgG抗体生成细胞数量以及泪液、气管液、肠液、胆汁的IgA、IgM、IgG含量均明显低于对照雏鸡,表明CIAV感染雏鸡后,其呼吸道和消化道局部粘膜的细胞免疫和体液免疫机能均下降,这与CIAV感染雏鸡对其它某些病原易感性提高密切相关。

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