成髓细胞的

GM-CSF had dual effects on the cell growth,it could inhibit the fibroblasts proliferation at concentration abov e 1mg/L while stimulate the cell growth at concentration between 1mg/L and 0 .001mg/L.

GM-CSF的浓度在0.001～1mg/L之间对牙周膜成纤维细胞、牙髓成纤维细胞有明显的增殖效应，大于1mg/L时对增殖有明显的抑制作用。

The notable proliferation was not observed by eyes in the local of injection. The infiltration of inflammation cells and mild proliferation of fibrocyte around dura mater was observed by HE stained in 4 and 8 weeks after injection. Infiltration and exudation of inflammation cells was observed by HE stained in epidural nerve root. Compared with group A, no changes of group B, C and D were observed under specific stained. Proliferation of type Ⅱ collagen fibers around dura mater was seen under immunohistochemical stained in 4 and 8 weeks after injection. There is no significant demyelination changes under LFB stained. The thickness and shape of the myelin sheath in epidural nerve root was not regular under transmission electronic microscopy in 4 and 8 weeks after injection. Fibroblast was also seen there. In nerve endometrium, macrophage could be seen under TEM, myelinated nerve fiber changed significantly, but nonmyelinated nerve fiber changed mildly. When 8 weeks, the changes of group D is smaller than the group B and C.

给药局部肉眼观察未见明显的纤维组织增生；HE染色可见B、C、D三组给药后四周及八周时硬膜内外均有炎细胞浸润，纤维细胞轻度增生，硬膜外神经根内有炎细胞浸润及炎性渗出；特殊染色B、C、D三组同A组相比未见有脊髓及神经根的改变；免疫组化染色，给药后四周及八周时，硬膜内外均有Ⅱ型胶原纤维增生；固兰染色B、C、D三组未见有明显脱髓鞘改变，与A组相比无明显异常改变；电镜观察B、C、D三组在给药后的四周及八周时，表现为硬膜外神经根内髓鞘厚薄不一，形状不规则，可见成纤维细胞，神经内膜中可见有巨噬细胞；粗大的有髓神经纤维变化明显，无髓神经纤维受累较轻；八周时电镜下D组改变较B、C两组为轻。

An immature cell of the bone marrow that is the precursor of a myelocyte.

成髓细胞，原始粒细胞骨髓的一种未成熟细胞，是髓细胞的前身

HDPCs are dominating cells in dental pulpal tissue. HDPCs can not only synthesize dental pulp organic ingredient, but also differentiate into odontoblast and protect dental pulp.

HDPCs是牙髓组织中的主体细胞，HDPCs不仅具有合成牙髓有机质的能力，而且必要时还能分化为成牙本质细胞保护牙髓。

2The distribution of HIF-1 a and COX-2 was unequal;.(3)These findings indicate that HIF-1a and COX-2 may be two important factors in pulpal lesions and there is a significantly hypoxia condiction in inflamed pulp .(4)The increase of HIF-1 a is a possible mechanism for hypoxia preconditioning .(5)Our results suggested that pulpal fibroblasts as well as macrophages might be involved in the pathogenesis of irreversible pulpitis.

和COX一2的表达均有不同程度的变化，在牙髓炎病程中可能发挥着重要的生理和病理作用，③在牙髓炎进程中和牙髓自身修复过程中存在着缺氧环境和缺氧耐受机制，④牙髓成纤维细胞有可能与单核巨噬细胞一同参与牙髓炎的病理进程，且具有修复和免疫功能。

The COX-2 positive cells were odontoblasts, pulpal fibroblasts vascularendothelial cells and macrophages (positive neutrophils could be found in some cases of the second group and of the third group).(3)We found that COX-2 developed up with HIF-1 a in third group ,but against it infourth group.

COX-2表达阳性的细胞有成牙本质细胞、成纤维细胞、血管内皮细胞和单核巨噬细胞(阳性中性粒细胞可出现在第二组和第三组)，HIF-1α与COX-2的表达相关主要在第三组和第四组牙髓标本中体现明显，在第三组二者的变化大致呈平行关系；在第四组基本呈反向变化关系。

AIM: Investigation of bio-effects of GM- CSF on periodontal ligament and pupal fibroblasts.

目的：观察GM-CSF对牙周膜成纤维细胞、牙髓成纤维细胞的生物学作用。

In both inflammatory and healthy gingival epithelium, CD14 mRNA positive cells, which are mainly located at thornlike cell layer, were found.

另一方面，牙髓组织除树突状细胞外CD14表达细胞很少，牙龈组织皮下的成纤维细胞和未分化细胞也不表达CD14。

R ESULT S: Strong specific staining for Smad1,4,5 was detected in the odont oblasts of normal and carious teeth. Weaker staining was observed in the u nderlying cell-rich layer and pulp core.

结果：Smad1，4，5在正常和龋坏牙髓的成牙本质细胞层呈强阳性表达，在下方的富细胞区及牙髓中心部位为阳性或弱阳性表达，炎症牙髓浸润的炎细胞中Smad1，4，5呈强阳性表达。

A study has indicated the expression of Smad2 in human dental pulp cells, but there are no reports about the functions of Smad2/3 in TGF- P modulating dental pulp repair and tooth development.

本研究的目的是观察人牙髓细胞内Smad2和Smad3分子在TGF-β1信号转导中的作用，以及TGF-β1对二者表达水平的调控方式，并检测Smad2和Smad3在牙胚发育的不同时期和正常、龋坏、炎症牙髓组织中的表达及其变化，旨在从细胞内信号转导水平探讨TGF-β调节成牙本质细胞分化和牙本质形成的分子机理，为揭示牙胚发育和牙髓损伤修复过程中基因表达的调控机制提供一定的实验依据。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。