成骨细胞的

BACKGROUND: Seed cells and supporting materials are two key problems for cartilaginous tissue engineering.

背景：软骨组织工程的两个关键问题是种子细胞和支架材料，保证支架材料上有足够数量的功能细胞是体内形成骨和软骨组织的基础。

BACKGROUND: The basic fibroblast growth factor has the obvious promotion function of multiplication of the mesoderm and nerve ectoderm cells, and has the indirect ossification to the bone marrow-derived mesenchymal stem cells.

背景：碱性成纤维细胞生长因子对来源于中胚层和神经外胚层的细胞具有明显的促进增殖作用，对骨髓间充质干细胞具有间接的成骨作用。

Raman microspectroscopy was applied to monitor hydroxyapatite associated with the differentiation of the human mesenchymal stem cells to osteoblast.

在人体间叶干细胞分化成骨母细胞时，可利用拉曼光谱仪，对不同时期的骨母细胞做监测；最主要的差异是氢氧基磷灰石(Hydroxyapatite， HA)强度的表现。

MAIN OUTCOME MEASURES: The morphology of BMSCs was observed by the inverted phase contrast microscope. Expression of BMSC surface antigens was detected by flow cytometer. Osteogenous potential was assessed by alkaline phosphatase staining. Adipogenic potential was evaluated by Oil red O staining. Chondrogenic potential was assessed by toluidine blue staining.

主要观察指标：倒置相差显微镜下观察骨髓间充质干细胞的生长状态，流式细胞仪检测细胞表面标志物的表达，采用碱性磷酸酶染色鉴定成骨能力，以油红O染色鉴定成脂能力，以甲苯胺蓝染色鉴定成软骨能力。

Spindle shape of cells gradually disappeared, with increased cell body. Some cells grew in cluster. MSCs changed figures to multilayer knots 10 days after inducing osteoplastic differentiation.

骨髓间充质干细胞向成骨分化过程中，纺锤形的突起逐渐消失，胞体增大，培养10 d后，部分细胞呈聚集生长，随细胞生长密度的增长形成多层的结节结构。

MAIN OUTCOME MEASURES: Morphological changes in primary cultured and subcultured mouse BMSCs were measured. BMSC surface antigen of the second passage of mice (CD105, CD44, CD25, CD34) were determined using flow cytometry. The modified method was assessed to harvest the purity of stem cells. Activity of mouse BMSCs was identified using adipogenic and osteoplastic differentiation. The strength of fluorescent cells following multiple passage was observed.

主要观察指标：原代及传代小鼠骨髓间充质干细胞形态变化；对第2代小鼠骨髓间充质干细胞表面抗原，CD105，CD44，CD25，CD34进行流式细胞仪检测，评价改良方法获取干细胞纯度；通过成脂成骨分化，鉴定小鼠骨髓间充质干细胞的活性；观察多次传代后荧光细胞强度。

Adipose tissue-derived stromal cells have osteogenic activity after induction culture, which are able to repair bone defects combined with bovine decalcified bone.

脂肪基质细胞经诱导培养具备成骨活性，与细胞支架复合具备修复骨缺损的能力。

BMSCs cultured in vitro contain osteogenic precursor cells, and the passaged cells possess osteogenic potential.

体外分离培养的BMSCs中含有骨源性前体细胞，传代细胞具有较强的成骨潜能。

This study will contribute to a better understanding of mechanism of bone remodeling during tooth movement which may set the basis for clinical work. Methods1. Observe the expression of ATF4 protein in periodontal tissues after orthodontictooth movementA titanium-nickel closed-coil spring was applied to the occlusal surface of the rat maxillary first molar (M1) with a hook and the upper incisors with a ligature wire.

但正畸骨改建与骨发育是两种不同的生理过程，ATF4在正畸牙齿移动牙周组织的表达和分布，在机械力的作用下体外培养的人牙周膜细胞中表达变化的情况，以及在牙周膜细胞向成骨样细胞分化的过程中所起的作用，国内外尚未有报道。

The induced BMSCs with Dex、β-Gp and AA is a reliable tool for the bone tissue engineer .

骨髓基质细胞作为构建组织工程骨的种子细胞在地塞米松、β-甘油磷酸、L-坏血酸诱导下成骨具有可靠的重复性。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐