成骨细胞的

The Effects of ED -71 on proliferation and differentiation were detected by MTT,.cell cycle, alkaline phosphatase activity and level of osteocalcin.

采用细胞形态学，碱性磷酸酶染色，培养细胞匀浆上清测定单位蛋白下的碱性磷酸酶活性，培养基中骨钙素含量分析及原位杂交方法检测ED-71对OS-732人成骨样细胞分化的影响。

Adipocytes in bone marrow cavity could decrease the activity of ALP, weaken the expression of Collagen Ⅰ mRNA, inhabit osteogenic potential of bone marrow stromal cell, and induce apoptosis of bone marrow stromal cells.

髓内脂肪细胞可降低骨髓基质细胞内ALP活性，减弱CollagenⅠ mRNA表达，干扰其成骨能力，并能诱导骨髓基质细胞发生凋亡，在骨质疏松症的发病中有着重要的意义。

All of above were higher than those of control side.Conclusions ADM can improve the bone quality, increase bone density and intensity and is an ideal guided bone regeneration membrane for alveolar ridge agumentation with TiNi-SMA distraetor.

脱细胞真皮基质作为屏障膜，能提高牵张区成骨质量，增加骨强度，在镍钛记忆合金牵张成骨过程中是一种理想的引导骨再生生物膜。

Bone induction is an important theory in the early 20th century. Urist found that bone morphogenetic protein could promote mesenchymal cell to differentiate into osteoprogenitors and chondroblast in 1965, thence many scholars went in for the significant work in this research field.

诱导成骨是近代发展的重要理论，Urist首先提出间充质细胞在骨形态发生蛋白的作用下可向成骨和成软骨方向分化，此后国内外很多学者在该领域进行了广泛深入的探索。

TGF-βand IGF-1 acted as an important role in every stage of fracture healing, the change significantly in the endochondral bone may delay the endochondral bone formatted.

骨质疏松骨折愈合过程中TGF-β IGF-1的细胞在软骨成骨期表达的显著变化，可能导致软骨成骨延缓，延迟了骨折愈合。

The procedure could provide firm internal fixation,improve activity of osteoblasts in fractural sites.accelerate fracture healing.

上述方法可提供早期坚强的内固定，补充成骨基质和成骨前质细胞，改善成骨能力。

The passage 3 rat bone marrow mesenchymal stem cells were cultured at the density of 5×107/L in different groups, the induced group was added into conditioned medium containing 1×10-8 mol/L dexamethasone, 10 mmol/L beta-sodium glycerophosphate and 50 mg/L ascorbic acid at the same time of seeding, at 50%-60% confluence, at 85%-90% confluence, respectively.

将第3代大鼠骨髓间充质干细胞以5×107 L-1密度分组培养，诱导组分别于接种当时、细胞达50%~60%融合时、细胞达85%~95%融合时加入含地塞米松、β-甘油磷酸钠、维生素C的成骨诱导培养基诱导。

For ADSCs at passage 3, the formation of red l ipid droplets within cells was observed 2 weeks after being induced towards adipocyte, red calcium nodules were evident 3 weeks after being induced towards osteoblast, and highly congregated cell mass positive for alcian blue staining appeared 2 weeks after being induced towards chondrocyte.

第3 代ADSCs 成脂诱导2 周，可见细胞内有红色脂滴形成；成骨诱导3 周，可见红色钙结节样改变；成软骨诱导2 周，可见高度聚集生长的细胞团，阿利辛蓝染色呈蓝色。

Allogeneic and syngeneic fresh bone, autolyzed antigen-extracted bone, bone matrix gelatin, demineralized bone matrix were implanted into the muscle pouch of mice, at different times after implantation, the immunological, histological and alkaline phosphatase assay were conducted. The results revealed that all four kinds of allogeneic implants activated specific cellular and humoral immune responses, most notably in fresh bone group, the AAA, BMG and DBM inhibited the proliferation of the lymphocytes in vitro and BMG had the most powerful inhibiting action, allogeneic AAA, BMG and DBM might induce heterotopic osteogenesis in vivo, however, there were obvious difference in ALP and histomorphometry between allografts and isografts.

在小鼠肌肉内植入同种异体或同系新鲜骨、自消化抗原去除骨、骨基质明胶、脱钙骨基质，术后免疫学、碱性磷酸酶、组织学检查发现：4种同种异体植入物均可引发特异性细胞免疫反应和体液免疫反应，其中以新鲜骨移植引发的免疫反应最为显著；自消化抗原去除骨、骨基质明胶、脱钙骨基质在体外具有抑制淋巴细胞增殖的作用，骨基质明胶对淋巴细胞增殖的抑制作用最显著；同种异体与同系4种植入物在体内成骨活性有显著差异；自消化抗原去除骨、骨基质明胶、脱钙骨基质均有异位诱导成骨活性，其中骨基质明胶骨诱导活性最佳。

Methods: firstly,depanding on the methods of fcm,mtt etc,we got the best density and actiontime of adriamycin.and then,we used several depressors of different signal transduction pathways to observe the possible pathway of osteosarcoma cell apoptosis.finally, we observed different expression including phosphorylation and non phosphorylation of correlated signal molecules,by the methods of westernblot.

首先通过fcm，mtt等方法探讨引起成骨肉瘤细胞凋亡的最佳药物作用浓度及时间；在此条件下应用不同信号转导通路的抑制剂观察导致细胞凋亡的可能通路；最后应用western blotting来检测在成骨肉瘤细胞凋亡过程中相关信号分子的表达水平及磷酸化水平的变化。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐