成骨细胞的

In conclusion ,the results of this study showed that the inducing agent HMBA could effectively inhibit the proliferation activity of the human ostosarcoma MG-63 cells,cause the arrest of G0/G1 phase, reverse the malignant phenotype characters,upregulate expression of the osteoblat-like phenotype markers including collagen,osteocalcin,osteonectin , facilitated the formation of bone nodule .

本研究的结果表明HMBA能够有效抑制人成骨肉瘤MG-63细胞增殖活动，改变肿瘤增殖核抗原表达，将细胞周期阻滞在G0/G1期，逆转人成骨肉瘤细胞恶性表型特征，在促进终末分化标志物Ⅰ型胶原纤维蛋白、骨钙蛋白、骨粘素的表达同时，促进骨结节生成，从而说明HMBA能够诱导人成骨肉瘤MG-63细胞终末分化，并且Rg1、BPO-L、Rg1+BPO-L以及Rg1+HMBA等均具有终末诱导分化能力。

In order to avoid prolin residue inhibiting enterokinase cleavage, 9bp of MGF cDNA 5′ end sequence was truncated by primer, then the obtained truncated MGF des(1-3MGF cDNA (321 bp) was subcloned in pET32a vector to construct a prokaryotic recombination expression plasmid.

通过RT-PCR从拉伸刺激的人成骨细胞中克隆MGF cDNA序列，并去除5'端9 bp的序列，使N端缺少对肠激酶(Enterokinase， EK)具有抑制作用的脯氨酸，将截短型MGF des(1-3 MGF cDNA序列克隆入pET32a质粒，构建重组表达质粒。

However, by removing the effects of proliferation from the experiment, AMSCs no longer out-performed BMSCs in their ability to undergo osteogenic and adipogenic differentiation.

然而，在实验中排除细胞的增殖作用，AMSC没有比BMSC的成骨和成脂的分化能力更强。

METHODS: From January 2000 to May 2002, 52 cases with various types of bone defect were treated with tissue engineered bone, which was constructed in vitro by allogeneous osteoblasts from periosteum 1 x 10(6/ml with bio-derived bone scaffold following 3 to 7 days co-culture. Among them, there were 7 cases of bone cyst, 22 cases of non-union or malunion of old fracture, 15 cases of fresh comminuted fracture of bone defect, 4 cases of spinal fracture and posterior route spinal fusion, 3 cases of bone implant of alveolar bone, 1 case of fusion of tarsotarsal joint.

2000年1月～ 2002年1月，用同种异体骨膜来源的成骨细胞 1×106 / ml与生物衍生骨支架材料构建的组织工程骨，经体外培养 3～ 7天后，植入修复52例各种类型的骨缺损，其中骨囊性病变7例，陈旧性骨折不愈合或畸形愈合 2 2例，新鲜粉碎性骨折伴骨缺损 15例，脊柱骨折后路植骨融合术4例，牙槽骨植骨 3例，足跗中关节植骨融合 1例。

To investigate the effect of Jiangu granula on the apoptosis of newborn rat calvaria osteoblast induced by TNF-ɑ in vitro.

目的 探讨健骨颗粒含药血清对TNF-ɑ体外诱导的新生大鼠成骨细胞凋亡的影响；进一步揭示健骨颗粒改善骨质疏松模型鼠骨量的可能机制。

Objective: To investigate the effects of recombinant human insulin-like growth factor on the proliferation , apoptosis and anabolic function of cultured rat osteoblasts in vitro.Method: Rat calvaria cells were cultured in medium with or without rhIGF-I.

目的 通过观察重组人胰岛素样生长因子I对体外培养的大鼠成骨细胞增殖、凋亡和功能的影响，探讨rhIGF-I对骨代谢影响的可能机制。

Miyamoto A, Shigematsu T, Fukunaga T, et al. Medical baseline data collection on bone and muscle change with space flight［J］. Bone, 1998, 22(5):7982.［2］ Otto F, Thoenell AP, Crompton T, et al. Cbfα1, a candidate gene for cleidocranial dysplasia syndrome, is essential for osteoblasts differentiation and bone development ［J］.

讨论 FSS对成骨细胞功能的影响骨骼按遗传基因的调控建设骨骼的雏形，并在骨的生长发育中根据所承受载荷的需要对自身不断地进行改建与重塑，以试图实现用最小的骨量达到最大的骨强度，这就是骨的功能适应性。

The present study has demonstrated the pattern of TNF-α and IL-6 expression, has defined the effect of cellular immunity on BMP-induced osteogenesis at the implant site, and has clarified the immunoregulation by BMP subsequent to bone xenografting, thus opening up a new field of molecular immunology associated with bone xenograft, allowing for the search for a more ideal approach to preventing immune rejection and developing a highly effective xenogenous graft material.

本研究结果揭示了异种骨移植局部TNF-α和IL-6的定位表达及其规律，基本上阐明了移植局部细胞免疫对BMP诱导成骨作用的影响及BMP在异种骨移植局部的免疫调节作用等问题，开辟了异种骨移植分子免疫学新领域，并为选择更理想的移植排斥防治途径和研制高效的异种骨移植材料提供了可靠的实验及理论依据。

Osteoblasts isolated from the cranium of Zelanian rabbit,after being transfected with green fluorescent protein,were inoculated onto the bio-derived bone to investigate the 3D fabrication of engineering bone in rotating wall vessel bioreactor.

用经绿色荧光蛋白标记的新西兰兔颅骨来源的成骨细胞复合到生物衍生骨支架材料上，在新型旋转壁式生物反应器内三维构建组织工程骨，同时与静态培养环境中构建的结果进行比较。

The present paper is to study the effect of insulin on the biological activity of osteoblasts obtained from diabetic rats mandibles,and to provide feasibility evidence for the local application of insulin via jaw bone.

本文将研究胰岛素对体外培养的糖尿病大鼠下颌骨成骨细胞生物学活性的影响，为胰岛素的局部植入式给药提供理论依据。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐