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Objective: To observe the effect of drug serum with No1 Guyu on skeletogenous cell proliferation and differentiation.

目的:观察中药骨愈1号药物血清对成骨细胞增殖、分化以及矿化的影响。

To date, clinic results of interaction between bone marrow stromal cells and Hydroxyapatite/Tricalcium phosphate compound are not enough.

通过骨髓基质细胞的原代分离、培养,观察其扩增性和成骨潜能;(2)。

TNF-α can induce apoptosis of osteoblast, which can be partly apposed by estrogen.

TNF-α可使成骨细胞产生凋亡,雌激素可部分对抗TNF-α的作用。

Results Histological investigation showed that the bone trabeculae were disarrayed containing dispersed cartilage cells in the denervated side, whereas the bone trabeculae disarryed orderly with rich blood vessels and no cartilage cell in the control side.

结果 实验侧牵张间隙骨小梁分布方向较杂乱,骨小梁内部有分散的软骨岛分布;对照侧牵张间隙骨小梁主要沿牵张方向分布,血管丰富,成骨细胞活跃。

Lysed cells were cultured for bacterium colony forming unit,unlysed cells were sent for EM examination.

部分被感染的成骨细胞溶解后用于细菌培养,计算菌落数,部分送电镜观察。

Effects of pulsing electromagnetic fields with different wave forms on proliferation of osteoblasts.

不同脉冲波电磁场对成骨细胞增殖的作用。

By histological examination, it was found that in HAC group, there were granulation tissue, fibrous tissue and newly formed vessels grew into the pores and the osteoblasts formed osseous trabeculae.

组织学显示在不同阶段,多孔HAC植片标本有肉芽组织、纤维组织和新生血管向微孔中生长及成骨细胞形成的骨小梁。

In addition, the effect of some flavonoid compounds on osteoblast-like cells was also studied.

此外,本研究考察了一些黄酮类化合物对成骨样细胞的作用。

Then to infect the rats BMSCs with the recombinant lentivirus in order to observe the differentiation of the infected cells.

用慢病毒感染大鼠骨髓间质干细胞后,观察其向成骨细胞分化的情况。

Methods: Proximal tibias from neonatal rats were collected. The protein expressions of VEGF and its receptors Flt-1 and KDR/Flk-1 in osteoblasts in proximal tibias of neonatal rat were detected by immunohistochemistry.

切取新生鼠胫骨上端组织,采用免疫组织化学染色方法,检测VEGF及其受体Flt -1和KDR/Flk -1在新生鼠胫骨上端成骨细胞中蛋白质的表达。

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