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The presence of calcium deposit activity in cultured cells was demonstrated by Van Kossa staining and alizarin red staining.

在常规培养方法的基础上进行改良,建立组织块漂浮培养法,从新生兔颅骨分离、培养成骨细胞。

MTT method was used to detect the proliferation of the cells. Cell alkaline phosphatase activity and expression of collagen type I were assessed.

另以组织化学和免疫组织化学方法检测传代培养的SD大鼠骨髓基质成骨细胞在PMMA驻极体表面的增殖和分化情况。

Objective: To explore the effects of Gubao-containing serum on ALP activity and OPG mRNA expression in osteoblasts.

目的:观察骨宝含药血清对SD大鼠成骨细胞中护骨素mRNA表达的影响。

The bone dosimetric values are influenced by the parameters of skeleton mass.

鉴于骨骼组织不同部位的辐射敏感性存在差异[2],特别是已确认骨髓的造血干细胞和衬在骨表面上的成骨细胞是骨骼中致癌危险的靶细胞,因而ICRP30号出版物[3]详细介绍了估算骨骼靶器官的吸收剂量的骨剂量学模型,使骨剂量的计算趋向精确。1995年,ICRP又专门编著了用于辐射防护的骨骼解剖和生理参数的70号出版

We have, therefore, investigated the direct effects of adiponectin on primary cultures of osteoblastic and osteoclastic cells in vitro and determined its integrated effects in vivo by characterizing the bone phenotype of adiponectin-deficient mice.

为此,我们研究了脂联素对离体培养的原代成骨细胞和破骨细胞的直接影响,并通过对脂联素缺陷小鼠的骨基因表型的研究明确其在体内的综合作用。

For normal adult male rat,calcitonin depresses the osteoclastic resorption obviously through reducing the number of active resorptive surface and depressing osteoclastic activity;on the other hand,calcitonin depresses the osteoid producing activity of osteoblasts by indirectly affecting the coupling process between resorption and formation.Calcitonin administered in short did not affect the bone volume.

降钙素对正常成年雄性大鼠松质骨而言,可以明显抑制破骨吸收,这种作用可能是通过减少活性吸收表面数量和降低破骨细胞的吸收功能来完成的;可以抑制成骨细胞形成类骨质的活性,这种作用可能不是直接的,而是通过降钙素对骨吸收的抑制作用进而影响了骨吸收与骨形成的之间的偶联而完成的;短期应用降钙素骨量无变化。

It is one of the key pathogeneses of postmenapausal osteoporosis that estrogen deficiency leads to the decrease of OPG、TGFβ expression and the increasing expression of some bone-resorbing cytokines as OPGL、M-CSF and TRAIL, then stimulates osteoclast differentiation and activity, which potentiates bone resorption and bone loss:(2) ICI182780 shows partial transactivating function in osteoblasts, so it should belong to a kind of selective estrogen receptor modulators .

雌激素缺乏可能导致HOB中OPG、TGFβ的表达减少,而解除了对OPGL、M-CSF和TRAIL等刺激OC生成和活化的细胞因子的抑制作用,OC的数量和活性增加,导致骨吸收增强和骨量丢失,这是导致绝经后骨质疏松症的一个重要的发病机制;ICI182780在成骨细胞内表现出促转录活性,因此它并非纯ER拮抗剂,而应属于选择性雌激素受体调节剂(selective estrogen receptor modulator,SERM)。

In the period of 8 to 16 weeks after operation,new bone was formed continuously, artificial bone gradually reconstructed to mature lamellar bone,bone trabeculae and medullary cavity,etc. Conclusion The preparation of CPC is simple.

结果 CPC植入后4周有新生软骨形成及未成熟的骨组织,可见大量的软骨细胞、成骨细胞、胶原组织及较多的小血管。8~16周新骨继续生成,人工骨逐渐改建为成熟的板层骨、骨小梁和髓腔结构。

Results There existed new cartilage and new immature bone tissue in the bone substitute in 4 weeks after operation, as well as mass of cartilageous cells,osteoblasts, collagen tissue and more small vessels were seen. In the period of 8 to 16 weeks after operation,new bone was formed continuously, artificial bone gradually reconstructed to mature lamellar bone,bone trabeculae and medullary cavity,etc.

结果 CPC植入后4周有新生软骨形成及未成熟的骨组织,可见大量的软骨细胞、成骨细胞、胶原组织及较多的小血管。8~16周新骨继续生成,人工骨逐渐改建为成熟的板层骨、骨小梁和髓腔结构。

In the whole life of human being, bone is always in the process of new-old alteration, which means osteoclast absorbing old sclerotin and osteoblast forming new sclerotin.

人的一生中骨骼一直处于新旧更替的过程中,即由破骨细胞吸收旧的骨质,成骨细胞形成新的骨质。

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