成骨细胞的

METHODS: The calvarium bone of newborn rats was digested with bacterial collagenase and cultured to obtain osteoblasts and SP with different concentrations was then added. The proliferation and activity changes of the osteoblasts were identified by counting the cells, determining the ALP activity and observing the changes of cell cycle.

采用新生大鼠颅盖骨消化法培养成骨细胞，再加入不同浓度的SP，观察细胞生长数量，测定碱性磷酸酶活性及观察细胞周期变化。

METHODS: calvarium bone of newborn rats was digested with bacterial collagenase and cultured to obtain osteoblasts and SP with different concentrations was then added. The proliferation and activity changes of the osteoblasts were identified by counting the cells, determining the ALP activity and observing the changes of cell cycle.

采用新生大鼠颅盖骨消化法培养成骨细胞，再加入不同浓度的SP，观察细胞生长数量，测定碱性磷酸酶活性及观察细胞周期变化。

The calvarium bone of newborn rats was digested with bacterial collagenase and cultured to obtain osteoblasts and SP with different concentrations was then added. The proliferation and activity changes of the osteoblasts were identified by counting the cells, determining the ALP activity and observing the changes of cell cycle.

采用新生大鼠颅盖骨消化法培养成骨细胞，再加入不同浓度的SP，观察细胞生长数量，测定碱性磷酸酶活性及观察细胞周期变化。

The calcified nodules were found at days 20 of primary culture in osteoblasts, with more cellular fission in growing period. Cell process was mutual linkage and arranged in shape of typical "cobble-stone" when reached a confluence.

成骨细胞原代培养20 d时出现不透光的钙化结节，生长期细胞分裂相多见，细胞突起相互连接，汇合时呈铺路石状。

2Cm bone defect was established in ulna of rabbits, the capacity of different therapeutic measures in bone defect repairment was determined in groups A , B (implanted BMP, TGF -β〓 and carrier) and C . It was found that the appearance of osteoblasts and formation of bone union were earlier and the number of osteoblasts more in group B than in group A. The amino acid analysis revealed that the volume of ossein was significantly higher in group B than in group A at the early stage.

分别用bBMP及载体bBMP、TGF-〓及载体和单纯载体修复兔尺骨1.2cm骨缺损的实验模型来比较三者间的成骨效果，结果表明B组比A组成骨细胞出现早、形成数量多、骨连接形成提前，骨胶原氨基酸分析在骨缺损修复早期胶原形成量B组均高于A组，且B组以形成Ⅰ型胶原为主，C组表现为一纤维形成过程。

Osteogenesis can be observed in vitro and in vivo used osteoblast of different sources composite with s...

用不同来源的成骨细胞与不同组成成分和结构的支架复合，在体内和体外都观察到成骨现象。

Osteogenesis can be observed in vitro and in vivo used osteoblast of different sources composite wit...

用不同来源的成骨细胞与不同组成成分和结构的支架复合，在体内和体外都观察到成骨现象。

During the process of osteophyte formation mesenchymal cells covering the periosteal bone surface proliferate and differentiate into chondroblasts and osteoblasts, which build up cartilage and bone matrix.

在骨赘形成过程中，覆盖骨膜骨的间充质细胞增生、分化为软骨母细胞和成骨细胞，后两者构建了软骨和骨基质。

METHODS: The rib trabeculae were resected and broken, trypsinizated and washed completely by PBS. Bone surface and non-adhesive floating cells in cleaning fluid were observed with inverted microscope. Rib trabeculae was washed by DMEM culture medium once, and cultured in culture bottle. The culture liquid was replaced by new one once a week. The osteoblast was moved from the sclerite a week later. The cells were fused monolayer and could be subcultured 4 to 6 weeks later.

切下人肋骨骨小梁剪碎，胰酶消化后以PBS彻底冲洗直至于倒置显微镜下观察骨片表面及清洗液中无粘着及漂浮的细胞，以DMEM完全培养液清洗骨小梁1次，移入培养瓶内培养，每周更换培养液1次，1周后可见成骨细胞自骨片移出，4~6周细胞融合成单层可作传代培养。

Abstract］objective: effects of a single period of mechanical strain on gene expression patterns of two osteogenic transcription factors, ets1 and cbfa1, in rat calvarial sutural osteoblast-like cells were studied in this study to elucidate the molecular mechanism of suture distraction.

摘要］ 目的：探讨大鼠颅骨缝成骨细胞在张应力作用下ets1和cbfa1基因表达的变化，以揭示骨缝牵张成骨的分子机制。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。