成骨细胞的

In addition, after stably expression of bFGF, the biological characteristics of BMSCs and bone marrow-derived osteoblasts were analyzed in the fields of morphology, proliferation, cell cycle, alkaline phosphatase, typeⅠ collagen and so on.

从形态、增殖特性和细胞周期、碱性磷酸酶、Ⅰ型胶原等方面分析稳定表达碱性成纤维细胞生长因子的骨髓间充质干细胞和骨髓源性成骨细胞生物学特性。

METHODS: The calvarium bone of new born rats was digested with bacterial collagenase and cultured to obtain osteoblasts and SP with different concentrations was then added.

采用新生大鼠颅盖骨消化法培养成骨细胞，再加入不同浓度的SP，观察细胞生长数量，测定碱性磷酸酶活性及观察细胞周期变化。

Objective To investigate the role of hypoxia inducible factor-1α(HIF-1α) and von Hippel-Lindau in murine endochondral ossification.

目的 研究低氧诱导因子1α(HIF-1a)和von Hippel-Lindau在成骨细胞水平对小鼠软骨内成骨过程的调控机制。

In intramembranous ossification, absence of cartilage, osteoblasts are likely producing, and responding to, VEGF.

膜内成骨中，在无软骨出现的情况下，成骨细胞产生、应答VEGF。

Results High homogenous hMSCs were obtained and after co-culture for 5 d with osteoblasts, they fused to form multinuclear cell clusters in micrograph and were postive in TRAP staining. Conclusion The co-culture system method is successful.

结果：体外分离、培养的hMSCs具有独特的细胞免疫学表型，即CD73和CD105阳性， CD34和CD45 阴性。hMSCs与成骨细胞共同培养5 d后，即可见单个核细胞融合成多核细胞，TRAP 染色可见多数细胞胞浆呈酒红色，为诱导成功的破骨细胞。

OBJECTIVE: To investigated the effect of simvastatin on osteoblastic differentiation of bone marrow stromal cells based on genetics level.

目的： 从基因水平明确辛伐他汀在体外诱导大鼠骨髓基质干细胞向成骨细胞分化过程中对成骨相关基因表达谱的影响。

Results: Most of the cultivated periosteal-derived osteoblasts were triangle-like or shuttle-like cells,they growed and breeded rapidly,and had osteogenetic capability.

结果：培养的骨膜成骨细胞形态多为三角形或梭形，生长增殖迅速，具有成骨能力。

Methods Osteogenetic culture medium containing different concentrations of atorvastatin 10^(-6, 10^(-7), 10^(-8)mol/L and control were added in the murine bone marrow cell subculture. The proliferation of the stromal cells was observed, and alkaline phosphatase staining and examination were done to check the differentiation ability of bone marrow cells. Alizarin red staining was performed to evaluate the mineralization of the osteoblasts in culture.

分别将10^(-8)、10^(-7)、10^(-6)mol/L的阿托伐他汀和空白药物加入传代培养的小鼠骨髓基质细胞，通过细胞形态学观察、细胞增殖率检测、碱性磷酸酶检测和钙结节染色，比较药物在成骨细胞分化过程中对细胞增殖和分化的影响。

Excellent osteogenous and degrade property on the surface UMCHA.

证实UMCHA骨水泥表面培养成骨细胞具有较高的成骨活性

Objective To investigate the dose-effect effects of th ree relating bone growth factors, dexamethasone, recombinant human basic fibro blastic growth factor and recombinant human bone morphogenetic protein- 2 (rhBMP-2), on proliferation and differentiation of periosteal cells so as to provide experimental basis for their further application in bone tissue engineer ing.

骨膜细胞是骨修复、重建过程中重要的功能细胞，在一定条件下具备向成骨细胞分化，分泌骨细胞外基质的潜能，因此成为骨组织工程研究中构建细胞-材料复合体非常重要的种子细胞。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。