成骨细胞的

objective to ivestigate the effect of serum of astragalus from sd rats on the osteogenic capacity of calvarium osteoblast in vitro.

摘 要 目的观察黄芪含药血清对新生大鼠颅骨成骨细胞体系增殖、分化及成骨能力的影响。

Many investigations make clear that Osteoelast, prosoma of Osteoclast, Osteoblast all can express NOS. NOS activation receive the induction of Ca2+, Calmodulin, cytokine and endotoxin.

许多研究表明破骨细胞及破骨细胞前体、成骨细胞均能表达一氧化氮合成酶，NOS的激活受Ca~(2+)和钙调蛋白或细胞因子和内毒素诱导。

The third passage of cells at 2×109/L were incubated in adipogenic, osteogenic and chondrogenic mediums, separately.

收集第3代细胞，分别接种于向脂肪细胞、成骨细胞、软骨细胞分化的培养液中进行诱导。

Methods Rat calvaria osteoblasts were cultured in the presence of 10 μg/mL-500 μg/mL nHA or nAg-HA/TiO2 for 2 h, 6 h, 8 h, 24 h, 72 h and 120 h. Cell proliferation, vitality and ultrastructure were tested using cell counting chamber, MTT assay, and transmission electron microscope.

将成骨细胞分别在添加了不同浓度(10μg/mL~500μg/mL)的nHA或nAg-HA/TiO2的培养基中培养2、6、8、24、72及120h，通过透射电镜观察、细胞计数及MTT法检测细胞超微结构、细胞增殖及线粒体活性，并计算单位细胞呼吸率。

In resent year, several in vitro models for committed differentiation of ES cells have been described which give rise to a variety of cell types, including hematopoietic, neuronal, chondroitic, osteocytic, endothelial, myogenic, cardiac and smoothes muscle cells, but the committed differentiation into epidermal stem cells have not been reported.

ES细胞为研究基因功能、胚胎发生和个体发育，及组织细胞分化机理提供了理想的模型。近年来的研究表明，适当条件下，ES细胞可定向分化为三个胚层的各种类型的细胞，其中包括向神经元、造血细胞、成骨细胞、心肌细胞、血管内皮、软骨细胞、平滑肌特方向的定向分化。

CT displayed the structure of femoral head was vague. A piece of coloboma could be observed clearly on the cartilage of a femoral head. Medullary canal was almost filled with white adipose tissue. Osteoblasts and osteocytes decreased. Empty bone lacuna increased. Thrombus distributed widely in the bone tissue microcirculation.

MA组病理检查肉眼可见股骨头软骨面局部缺损，坏死面暴露，髓腔内大量脂肪组织堆积；光镜下成骨细胞、骨细胞均减少，空骨陷窝增多，微小血栓广泛分布，变形扩大的脂肪细胞挤压正常骨髓组织细胞，骨小梁变细破裂明显。

Results D Cell morphology : Osteoblasts adhered tightly to five different titanium surface; On G surface, osteoblasts were spindle-shaped along the groove; Osteoblasts were cubic, multangular and abundant cytoplasm on SB surface and TPS surface, spreading in all directions.

①细胞粘附形态：成骨细胞在五种不同处理钛片表面粘附生长，SB组、TPS组表面细胞为多边形，胞浆丰富，有多个伪足突起呈分化表型；G组表面细胞胞体沿打磨沟嵴的方向伸展呈长梭形，少见伪足突起。

Methods BMSCs isolated from rabbit iliac bone marrow were culture-expanded in vitro. The injectable tissue-engineered bone constructed from autologous PRP, FG, and BMSCs was cultured in vitro, and its biological characteristics were observed including the time of gel formation, histological features, seed cell survival and microscopic structures. Results The constructed injectable tissue-engineered bone began gel formation within 20 to 30 s, and after a week-long culture, the gelatine began to degrade, and numerous well viable fusiform cells could be seen to adhere to the bottom of the Petil dish, Scanning electron microscopy identified globular and olivary cells embedded in the fibrin glue, and numerous small particles could be seen around of the cells.

从兔髂骨处抽取骨髓体外培养BMSCs并诱导向成骨细胞分化，抽取兔自体动脉血提取PRP，以FG、BMSCs、PRP共同构建可注射型组织下程骨并体外培养观察其生物学特性如凝胶形成时间，组织学特点、细胞存活情况及其超微结构特征等结果构建的可注射型组织工程骨可在短时间内形成凝胶，体外培养1周时其中细胞生长良好，电镜下见纤维蛋白网格结构致密，种子细胞及血小板颗粒分布广泛。

Now it is accepted that osteoblasts, in addition to being directly responsible for bone formation, also indirectly control bone resorption by mediating osteoclastic precursor cells differentiation and mature osteoclasts function.

现在普遍认为成骨细胞除了具有骨形成的直接功能以外，还能够通过介导破骨细胞前体细胞分化和成熟破骨细胞活化过程间接控制骨吸收。

The third passage osteoblasts were plated at the density of 5×104/ml in 75ml culture flask, twenty four hours later treatment factors were added.

许多激素及细胞因子通过调节破骨细胞和成骨细胞这两类细胞的数量和活性，使骨量维持动态平衡。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。