成骨细胞的

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞，运用活细胞计数和碘化丙吮检测其增殖能力；采用流式细胞术检测其细胞表面标志的表达；用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化，并用Von Kossa染色进行鉴定；用地塞米松与胰岛素诱导其向脂肪细胞分化，并以油红O染色进行鉴定。

Cumulative data have been documented that mesenchymal stem cells can differentiate into osteoblasts, chondrocytes, adipocytes, tenocytes, myotubes, neural-like cells, hematopoietic-supporting stroma and other non-hematopoietic cells derived from mesoblast or ectoblast.

大量研究表明，体外培养的骨髓间质干细胞在合适的诱导条件下，可分化为成骨细胞、成软骨细胞、脂肪细胞、成肌细胞、成腱细胞和神经元样细胞等多种非造血组织特别是中胚层和神经外胚层来源的组织细胞。

According to the bone apposition events at the interface, we measured the osteoblast attachment rate with the hemocytometer, the osteoblast growth curve with modified MTT method, the ALPase activity and protein content of cellular layers with modified kinetic method and modified Coomassie'method respectively, and osteocalain content in the cell medium with RIA , at different levels of cell responses, as osteocompatibility indexs.

根据骨内种植体界面的骨愈合过程，分别从成骨细胞反应的不同层次选择了细胞贴壁率、细胞生长曲线、细胞层ALPase活性和蛋白质含量以及细胞培养液中骨钙素的含量作为体外评价材料骨性生物相容性的指标。采用细胞计数板计数法测定细胞贴壁率，采用改良MTT法测定细胞生长曲线。

Methods:(1) Bone marrow stromal cells isolated from Beagle dogs were cultured in vitro for the primary culture,in basic medium consisted ofDMEM^iew horning cattleerum,etc. Parts of subculture were cultured in mineralization medium which additionally contained 50 y g/ml ascorbic acid, 10mmol/LNa- beta -Glycerophosphate and 10-8mol/L dexamethasone in order to induce cells differentiating into osteoblasts.

①抽取毕格犬髂骨骨髓体外分离培养获得骨髓基质细胞，DMEM、新生牛血清培养基进行原代培养，部分传代细胞以含10~(-8)mol/L地塞米松、50μg/ml抗坏血酸和10mmol/Lβ—甘油磷酸钠的矿化培养液诱导其向成骨细胞增殖分化，用倒置相差显微镜进行细胞形态学和细胞生长增殖观察。

Methods Primary metanephric mesenchyme stem cells were cultivated and multi-directional differentiation was induced.

方法原代分离培养后肾间充质干细胞，在体外特定诱导条件下观察细胞向成骨细胞、脂肪细胞及肾小管上皮细胞的分化及其形态演变，采用细胞免疫荧光检测细胞表面特定蛋白的表达变化。

BACKGROUND: Under different induction conditions, bone marrow mesenchymal stem cells can differentiate into the mesodermal tissues such as osteoblasts, chondroblasts, muscle cells, adipocytes and so on.

背景：骨髓间充质干细胞在不同诱导条件下具有向中胚层组织细胞如成骨细胞、成软骨细胞、肌细胞、脂肪细胞等分化的能力。

Our data strongly suggest that some features of the osteoclastic phenotype--that are absent in pycnodysostosis--are a prerequisite for the anabolic effect of PTH on osteoblasts.

我们的数据强烈提示破骨表型的某些特征（注：此处我认为意译为破骨细胞的活化）是甲状旁腺激素增加成骨细胞数量及活性的先决条件，而这些特征是致密成骨不全症患者所不具备的。

Results Positive staining for VEGF was distributed mostly in osteoblasts, osteoclasts and newly-born osteocytes. Vascular endothelial cells and bone marrow cells also showed VEGF positive reaction. Hypertrophic chondrocytes in calcifying region of chondroephysis were VEGF positive, but VEGF was absent from chondrocytes in other zones of chondroephysis.

结果 VEGF阳性反应主要见于成骨细胞、破骨细胞及新生骨细胞中，血管内皮细胞及骨髓细胞也呈阳性反应；骺软骨钙化区肥大的软骨细胞为VEGF阳性反应，但其它区域的软骨细胞中无VEGF表达。

It is further confirmed by the experiment of muti-inducement that MSMPs can differentiate into osteocyte sand fibrocartilage cytes and it is osteo-chondro progenitor cells with the potential ability to evolve muti-differentiated. This added evidence to the view that condylar cartilage differentiated from osteo-chondro progenitor cells in the mesenchymal cells of mancibular periosteal.

体外分离培养鼠胚下颌体骨膜间质细胞并研究其生物学特性，通过多向诱导实验进一步证实：下颌骨膜间质细胞可能为骨—软骨的前体细胞，可分化为成骨细胞及纤维软骨细胞，具有多向分化潜能，此结果进一步支持髁突软骨是由下颌骨膜间质中骨—软骨前体细胞发育而来。

Fluid shear stress synergizes with insulinlike growth factorI on osteoblast proliferation through integrindependent activation of IGFI mitogenic signaling pathway［J］.

模拟失重对成骨细胞功能的影响我们前期研究发现，在FSS的作用下，1 G重力环境下培养的成骨细胞分泌的PGE2可迅速增加。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。