成骨细胞的

Objective: To study osteogenic capability of rabbit bone marrow stromal cells and biological characteristical identification in vitro in order to explore the appropriate methods of osteoblast culture in vitro and to select an idea source of seed cells for bone tissue engineer .

目的：研究兔骨髓基质细胞在体外培养条件下的成骨能力及生物学特性，探讨简便易行的成骨细胞体外培养方法，为组织工程选择理想的种子细胞来源。

We review the effect of rare earth ions on bone metabolism as the following three aspects:(1)effect of rare earth ions on differentiation and function expression of osteoblasts and osteoclasts;(2)effect of rare earth ions on the osteogenic and adipogenic differentiation of bone marrow stromal cells;(3)effect of rare earth ions on bone mineral phase of animals.

本文回顾了稀土离子对骨代谢影响的研究进展，探讨了其药用和安全性问题，主要从以下三个方面进行综述：(1)稀土离子对成骨细胞细胞和破骨细胞的分化和功能表达的影响；(2)稀土离子对骨髓基质细胞成骨分化和成脂分化的影响；(3)稀土离子对动物骨矿物相的影响。

Experimental study indicated: the flavone of rhizome drynaria can improve the cytoactive of marrow stroma cell of rats, promote its multiplicative division, and protect the marrow stroma cells' apotosis with lipidsion.The flavone of rhizome drynaria's serum can extently protect the osteoblast apoptosis of rats induced by TNF-α and the osteoblast senescence of rats induced by t-BHP.

实验研究表明：骨碎补总黄酮能够提高大鼠骨髓基质细胞的活性，促进其增殖分裂，并对脂质化大鼠骨髓基质细胞凋亡具有保护作用，而且骨碎补总黄酮含药血清对TNF-α诱导的大鼠成骨细胞凋亡和t-BHP诱导的大鼠成骨细胞衰老均具有一定的保护作用。

Bright pink gelatiniform liquid with mass ratio of sodium alginate and gelatin of 2:3 was prepared. Rabbit osteoblasts at 5×109/L were mixed with CaCl2 solution to form fruit jelly-shaped sodium alginate-gelatin/osteoblast gel. Critical-sized calvarial defects were created in diameter of 1.5 cm in 15 rabbits. After 1 week, cell/scaffold complex (0.5 mL) was implanted.

制备海藻酸钠与明胶质量比为2∶3的透明粉红色胶状液体，引入兔成骨细胞，细胞终密度为5×109 L-1，与CaCl2溶液混合，形成果冻样海藻酸钠-明胶共混体系/成骨细胞凝胶。15只兔颅骨均制备直径1.5 cm的极限缺损，1周后植入凝胶复合体0.5 mL进行修复。

Bright pink gelatiniform liquid with mass ratio of sodium alginate and gelatin at ratio of 2:3 was prepared. Rabbit osteoblasts with final concentration of 5×109/L were mixed with CaCl2 solution to form fruit jelly-shaped sodium alginate-gelatin/osteoblast gel. Critical-sized calvarial defects were created in diameter of 1.5 cm in 12 rabbits. After 1 week, cell/scaffold complex (0.5 mL) was implanted to repair the bone defect in the experimental group. There was no treatment in the control group.

制备海藻酸钠与明胶质量比为2∶3的透明粉红色胶状液体，引入兔成骨细胞，细胞终浓度为5×109 L-1，与CaCl2溶液混合，形成果冻样海藻酸钠-明胶共混体系/成骨细胞凝胶。12只兔颅骨均制备直径1.5 cm的极限缺损，1周后实验组植入凝胶复合体0.5 mL进行修复，对照组不植入任何材料。

However, whether the human fibroblasts behave in a similar way has not been reported to our knowledge. In the present study fibroblasts collected from human foreskin were cultured in vitro so as to investigate into the fibro blastic osteogenetic role as well as its mechanism.

本实验包括四个部分：1、人皮肤成纤维细胞体外培养成骨的实验研究。2、猪骨形态形成蛋白（pBMP的提取及生物活性测定。3、pBMP对成纤维细胞成骨影响的实验研究。4、成纤维细胞体外培养过程中骨钙素变化。

These findings indicate that the novel prepared triphasic ceramic composites support adhesion, proliferation and osteogenic differentiation of hMSC as well as osteoclastogenesis of human monocytes in co-culture with osteoblasts.

结果提示制备的三相陶瓷复合材料支持人骨髓间充质干细胞的黏附、增殖和成骨分化，以及人单核细胞与成骨细胞联合培养条件下破骨细胞形成。

Icariin, the main constituent of Epimedium sagittatum Maxim, and its metabolite icariside Ⅱ showed similar activity in cell proliferation. The total flavonoids from Viscum coloratum Nakai could stimulate cell proliferation and significantly promote cellular ALP activity. Daidzein and its derivatives exhibited no significant activity in cell proliferation.

结果发现：中药淫羊藿中的主要成分淫羊藿苷及其大鼠体内主要代谢物淫羊藿次苷Ⅱ具有类似的作用，可显著促进成骨样细胞的增殖；中药槲寄生总黄酮对成骨样细胞的增殖有一定的促进作用，并可显著提高细胞内ALP活性；大豆苷元及其衍生物对UMR106细胞的增殖作用不明显。

Transcription factors can control the transcription of their target genes when they bind to the DNA-binding sites on their promotors. They can transfer the messages of growth factors and so on to those effective molecules. Core binding factor al is such a kind of transcription factor, which can control the differentiation of osteoblasts peculiarly. They can induce the pro-osteoblasts or non-osteoblasts to synthesize and secrete most mineralize related proteins such as ALP, OC, OPN, BSP and so on, in order to differentiate to osteoblasts finally.

转录因子是一类可以通过与下游靶DNA结合，从而调控下游基因转录的分子，它们接受来自生长因子等方面的信号，再将这些指令具体地传递给直接执行功能的蛋白分子。cbfal是一种成骨分化特异性转录因子，可以诱导成骨前体细胞合成和分泌ALP、OC、OPN、BSP等矿化相关蛋白，向成骨细胞分化。

After that the specimens were digested by collagenase for 90 min, the supernatant was collected and centrifuged.

成骨细胞周期和细胞凋亡的检测成骨细胞以 IX 10VInl接种至 25Inl培养瓶，在含 15％胎牛血清的 199培养液中培养 3天。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐